Proteomics Core

蛋白质组学核心

基本信息

批准号：
10005398
负责人：
Lauren Elizabeth Ball
金额：
$ 15.32万
依托单位：
MEDICAL UNIVERSITY OF SOUTH CAROLINA
依托单位国家：
美国
项目类别：
财政年份：
2011
资助国家：
美国
起止时间：
2011-09-01 至 2022-01-15
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10005398
关键词：
Acetylation Acute Address Applications Grants Bioinformatics Biomedical Research Cells Centers of Research Excellence Complex Computer software Consultations Custom Data Data Analyses Detection Disease Enzymes Equilibrium Experimental Designs Faculty Funding Future Global Change Goals Grant Half-Life Human Resources Individual Infrastructure Institution Label Ligands Lysine Manuscripts Mass Spectrum Analysis Mediating Medical Mentors Mentorship Methodology Modification Mutation Nitrates Nutrient Oxidants Oxidation-Reduction Participant Peptides Pharmaceutical Preparations Phosphorylation Phosphorylation Site Post-Translational Modification Site Post-Translational Protein Processing Preparation Protein Chemistry Proteins Proteomics Research Research Personnel Research Project Grants Sampling Services Signal Transduction South Carolina Stress Sulfhydryl Compounds Techniques Technology Time Tissues Training Tyrosine United States National Institutes of Health Universities base career development data acquisition data archive design differential expression experience experimental study instrumentation investigator training mass spectrometer nitration phosphoproteomics protein crosslink protein expression protein function response skills success symposium

项目摘要

ABSTRACT The objective of Proteomics Core B is to provide state of the art mass spectrometry-based proteomic capabilities, dedicated analytical support, and mentorship to the investigators funded through the SC COBRE in Oxidants, Redox Balance, and Stress Signaling (Redox COBRE). Mass spectrometry is a powerful technology enabling unbiased discovery of the effects of drugs/ligands, genetic alterations, and disease on changes in protein expression, protein interactions, and post-translational modifications. Post-translational modifications encode the nutrient and stress status of the cell and mediate acute responses to ligand stimulation and changes in redox status by influencing protein function, half-life, and localization. The MUSC Mass Spectrometry Facility and Proteomics Center have an extensive array of instrumentation for protein characterization and expertise in applying proteomic technologies to biomedical research endeavors. During the last funding period acquisition of an Orbitrap Elite mass spectrometer, purchased through a shared instrumentation grant, enabled us for the first time to apply quantitative proteomic approaches to the detection of complex post-translational modifications. In ongoing collaborative projects with COBRE investigators and participants in the Redox Center, Proteomics Core B is utilizing the Orbitrap Elite to examine the global effects of altered redox status on protein expression, phosphorylation, acetylation, and thiol modifications and to characterize the sites of phosphorylation, S-glutathionylation, tyrosine nitration, and protein crosslinking on redox-sensitive target proteins. The specific aims of Proteomics Core B are: 1) to assist COBRE investigators with experimental design including sample preparation strategies and choice of specific proteomic approach, 2) to customize data acquisition and analysis to address the goals of the individual projects, and 3) to provide mentorship and training in the applications of proteomic technologies for future projects. Access to these capabilities and expertise to push the available technology forward is critical to the proposed research projects. The experimental capabilities and infrastructure established with COBRE investigators are made available to investigators at MUSC and other institutions in South Carolina that utilize the MUSC Mass Spectrometry Facility. Our goals are to provide COBRE investigators with access to instrumentation and expertise to advance their research and contribute to the success of our promising junior faculty.

抽象的蛋白质组学核心 B 的目标是提供最先进的基于质谱的蛋白质组学通过 SC COBRE 资助的研究人员的能力、专门的分析支持和指导氧化剂、氧化还原平衡和压力信号（氧化还原 COBRE）。质谱分析法是一种强大的技术能够公正地发现药物/配体、基因改变和疾病对人体的影响蛋白质表达、蛋白质相互作用和翻译后修饰的变化。翻译后修饰编码细胞的营养和应激状态并介导对配体的急性反应通过影响蛋白质功能、半衰期和定位来刺激和改变氧化还原状态。 MUSC 质谱设施和蛋白质组学中心拥有广泛的蛋白质仪器将蛋白质组技术应用于生物医学研究工作的表征和专业知识。期间最后一个资助期收购了 Orbitrap Elite 质谱仪，通过共享购买仪器资助，使我们第一次能够将定量蛋白质组学方法应用于检测复杂的翻译后修饰。在与 COBRE 研究人员正在进行的合作项目中氧化还原中心的参与者，蛋白质组学核心 B 正在利用 Orbitrap Elite 来检查全球影响改变蛋白质表达、磷酸化、乙酰化和硫醇修饰的氧化还原状态表征磷酸化、S-谷胱甘肽化、酪氨酸硝化和蛋白质交联的位点氧化还原敏感的靶蛋白。蛋白质组学核心 B 的具体目标是： 1) 协助 COBRE 研究人员实验设计包括样品制备策略和特定蛋白质组学方法的选择，2) 定制数据采集和分析以实现各个项目的目标，以及 3) 提供在未来项目中应用蛋白质组技术的指导和培训。访问这些推动现有技术发展的能力和专业知识对于拟议的研究项目至关重要。与 COBRE 研究人员建立的实验能力和基础设施可供 MUSC 和南卡罗来纳州其他机构使用 MUSC 质谱仪的研究人员设施。我们的目标是为 COBRE 研究人员提供仪器和专业知识，以推进他们的研究并为我们有前途的初级教师的成功做出贡献。