Orbitrap Mass Spectrometer

Orbitrap 质谱仪

基本信息

批准号：
8247428
负责人：
Lauren Elizabeth Ball
金额：
$ 60万
依托单位：
MEDICAL UNIVERSITY OF SOUTH CAROLINA
依托单位国家：
美国
项目类别：
财政年份：
2012
资助国家：
美国
起止时间：
2012-05-01 至 2013-04-30
项目状态：
已结题

项目摘要

DESCRIPTION (provided by applicant): This proposal is for the addition of a Thermo Scientific LTQ Orbitrap Velos ETD hybrid FT mass spectrometer to the Mass Spectrometry Facility at the Medical University of South Carolina. The facility has a 39 year history of offerin state of the art mass spectrometry capability advancing a wide range of biomedical research interests in the local research community. Over the past three years, the facility has provided mass spectrometric analysis and assistance to 77 different faculty investigators within the university and multiple NIH-funded investigators at other institutions. The most highly requested services offered by the facility are protein identification and the characterization of posttranslational modifications. For these purposes, the most heavily used instruments in the facility are the low resolution linear ion trap mass spectrometers. The facility lacks an instrumen with the combined sensitivity, resolution, and multiple modes of peptide fragmentation that are achievable with the LTQ Orbitrap Velos ETD mass spectrometer. In fact, there is not an Orbitrap mass analyzer in South Carolina. The improved mass accuracy of this instrument will provide a higher level of confidence in peptide and protein identifications and in the site-assignment of posttranslational modifications (PTMs). Combining multiple fragmentation options (CID, HCD, ETD) with high mass accuracy measurements will enable quantitative approaches aimed at simultaneous identification and quantitation of protein modifications, a capability which is not currently available to facility users. The ETD ionization source accessory will 1) aid in projects requiring the characterization of PTMs and de novo sequencing and 2) provide new capabilities enabling top to middle-down protein analysis and quantitation of modifications that are destroyed during CID. The requested instrument will serve a representative group of Major Users including eleven NIH-funded investigators conducting research in the areas of cancer, cardiovascular disease, diabetes, vision, osteoporosis, and kidney disease. The Orbitrap Velos ETD instrument incorporates two mass analyzers and multiple fragmentation strategies yielding a powerful and very versatile instrument that will be well suited to address the broad range of protein analyses and characterization requested by investigators at MUSC and the neighboring universities and research centers in South Carolina. PUBLIC HEALTH RELEVANCE: The analysis of proteins by mass spectrometry is a fundamental analytical method utilized in biomedical research aimed at understanding processes underlying the onset and progression of disease and developing new therapeutics and diagnostics. The requested instrumentation will enhance our ability to identify and monitor changes in protein expression and regulatory post-translational modifications thereby advancing research in multiple areas including cancer, cardiovascular disease, diabetes, blindness, neurological disease, and osteoporosis.

描述（由申请人提供）：该提案旨在为南卡罗来纳医科大学的质谱设施添加 Thermo Scientific LTQ Orbitrap Velos ETD 混合 FT 质谱仪。该设施拥有 39 年提供最先进的质谱能力的历史，推动了当地研究界广泛的生物医学研究兴趣。在过去的三年里，该设施为大学内 77 名不同的教职研究人员和其他机构的多名 NIH 资助的研究人员提供了质谱分析和帮助。该设施提供的最受欢迎的服务是蛋白质鉴定和翻译后修饰的表征。出于这些目的，设施中使用最频繁的仪器是低分辨率线性离子阱质谱仪。该设施缺乏一种具有 LTQ Orbitrap Velos ETD 质谱仪可实现的综合灵敏度、分辨率和多种肽裂解模式的仪器。事实上，南卡罗来纳州没有 Orbitrap 质量分析仪。该仪器改进的质量准确度将为肽和蛋白质鉴定以及翻译后修饰 (PTM) 的位点分配提供更高的可信度。将多种碎片选项（CID、HCD、ETD）与高质量准确测量相结合，将能够实现旨在同时识别和定量蛋白质修饰的定量方法，这是目前设施用户无法获得的功能。 ETD 电离源附件将 1) 帮助需要 PTM 表征和从头测序的项目，2) 提供新功能，实现从上到下的蛋白质分析以及对 CID 期间破坏的修饰进行定量。所请求的仪器将为主要用户的代表性群体提供服务，其中包括 11 名 NIH 资助的研究人员，他们在癌症、心血管疾病、糖尿病、视力、骨质疏松症和肾脏疾病领域进行研究。 Orbitrap Velos ETD 仪器结合了两台质量分析仪和多种裂解策略，是一款功能强大且用途广泛的仪器，非常适合满足 MUSC 以及南卡罗来纳州邻近大学和研究中心的研究人员要求的广泛蛋白质分析和表征。。公共健康相关性：通过质谱分析蛋白质是生物医学研究中使用的一种基本分析方法，旨在了解疾病发生和进展的过程并开发新的治疗方法和诊断方法。所需的仪器将增强我们识别和监测蛋白质表达和翻译后修饰调节变化的能力，从而推进多个领域的研究，包括癌症、心血管疾病、糖尿病、失明、神经系统疾病和骨质疏松症。

项目成果

期刊论文数量（5）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

The DNA-binding protein CST associates with the cohesin complex and promotes chromosome cohesion.

DNA 结合蛋白 CST 与粘连蛋白复合物结合并促进染色体粘连。

DOI：
发表时间：
2021
期刊：
影响因子：
0
作者：
Schuck, P Logan;Ball, Lauren E;Stewart, Jason A
通讯作者：
Stewart, Jason A

S-Glutathionylated Serine Proteinase Inhibitors as Biomarkers for Radiation Exposure in Prostate Cancer Patients.

S-谷胱甘肽化丝氨酸蛋白酶抑制剂作为前列腺癌患者辐射暴露的生物标志物。

DOI：
发表时间：
2019
期刊：
影响因子：
4.6
作者：
Zhang, Leilei;Zhang, Jie;Ye, Zhiwei;Manevich, Yefim;Townsend, Danyelle M;Marshall, David T;Tew, Kenneth D
通讯作者：
Tew, Kenneth D

Remodeling Translation Primes CD8+ T-cell Antitumor Immunity.

重塑翻译启动 CD8 T 细胞抗肿瘤免疫。

DOI：
发表时间：
2020
期刊：
影响因子：
10.1
作者：
Hurst, Katie E;Lawrence, Kiley A;Robino, Rob A;Ball, Lauren E;Chung, Dongjun;Thaxton, Jessica E
通讯作者：
Thaxton, Jessica E

Mapping Extracellular Matrix Proteins in Formalin-Fixed, Paraffin-Embedded Tissues by MALDI Imaging Mass Spectrometry.

通过 MALDI 成像质谱法绘制福尔马林固定、石蜡包埋组织中的细胞外基质蛋白图谱。

DOI：
发表时间：
2018-01-05
期刊：
影响因子：
4.4
作者：
Angel, Peggi M;Comte;Ball, Lauren E;Talbot, Kacey;Mehta, Anand;Brockbank, Kelvin G M;Drake, Richard R
通讯作者：
Drake, Richard R

In Situ Imaging of Tryptic Peptides by MALDI Imaging Mass Spectrometry Using Fresh-Frozen or Formalin-Fixed, Paraffin-Embedded Tissue.

使用新鲜冷冻或福尔马林固定的石蜡包埋组织，通过 MALDI 成像质谱法对胰蛋白酶肽进行原位成像。