Study of Allosteric Proteins by NMR

通过 NMR 研究变构蛋白

基本信息

批准号：
8068044
负责人：
ERIK R ZUIDERWEG
金额：
$ 18.41万
依托单位：
UNIVERSITY OF MICHIGAN AT ANN ARBOR
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-06-01 至 2011-05-31
项目状态：
已结题

项目摘要

DESCRIPTION (provided by applicant): Hsp70 (heat shock 70 kDa) chaperone proteins are central to protein folding, refolding, and trafficking in organisms ranging from Archae to Homo Sapiens, both at normal and at stressed cellular conditions. Hsp70's (re) fold proteins via binding and release cycles at the substrate-binding domain involving conformational changes caused by ATP and ADP binding at the nucleotide-binding domain. This remote regulation mechanism is called allostery. Recently, Hsp70's have been linked to diseases such as breast cancer. Modulation of the allosteric mechanism of the Hsp70's with small compounds may form an avenue to treat these diseases. We propose to investigate the mechanism in detail by determining the solution conformations of 60 kDa Hsp70 protein constructs, containing both nucleotide- and substrate-binding domains and their complexes with co-chaperones in different liganded states. We will investigate the molecular basis for the action of the first generation of Hsp70 modulating compounds. The combined insights gained will aid in the design of improved compounds for the treatment of cell-management diseases. The research will be carried out using ultra-high field nuclear magnetic resonance spectroscopy.

描述（由申请人提供）：HSP70（热休克70 kDa）伴侣蛋白在正常和应力的细胞条件下，在正常和应力的细胞条件下，蛋白质折叠，重折叠和运输的蛋白质折叠，重新折叠和运输。 HSP70（RE）通过结合和释放循环在底物结合域上折叠蛋白，涉及由ATP和ADP结合在核苷酸结合域上引起的构象变化。这种远程调节机制称为变构。最近，HSP70与乳腺癌等疾病有关。对HSP70的变构机制的调节可能会形成治疗这些疾病的途径。我们建议通过确定60 kDa Hsp70蛋白质构建体的溶液构象，其中包含核苷酸和底物结合结构域及其复合物与不同配合状态的共振蛋白。我们将研究第一代HSP70调节化合物作用的分子基础。获得的组合见解将有助于设计改进的化合物，以治疗细胞管理疾病。该研究将使用超高的磁场磁共振光谱进行。

项目成果

期刊论文数量（13）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Parameterization of peptide 13C carbonyl chemical shielding anisotropy in molecular dynamics simulations.

分子动力学模拟中肽 13C 羰基化学屏蔽各向异性的参数化。

DOI：
10.1002/cphc.200700003
发表时间：
2007
期刊：
Chemphyschem : a European journal of chemical physics and physical chemistry
影响因子：
0
作者：
Jordan,DanielM;Mills,KMaria;Andricioaei,Ioan;Bhattacharya,Akash;Palmo,Kim;Zuiderweg,ErikRP
通讯作者：
Zuiderweg,ErikRP

The 70-kDa heat shock protein chaperone nucleotide-binding domain in solution unveiled as a molecular machine that can reorient its functional subdomains.

溶液中的 70 kDa 热休克蛋白伴侣核苷酸结合结构域被揭示为一种分子机器，可以重新定位其功能子结构域。

DOI：
10.1073/pnas.0401313101
发表时间：
2004
期刊：
Proceedings of the National Academy of Sciences of the United States of America.
影响因子：
0
作者：
Zhang,Yongbo;Zuiderweg,ErikRP
通讯作者：
Zuiderweg,ErikRP

Eta(z)/kappa: a transverse relaxation optimized spectroscopy NMR experiment measuring longitudinal relaxation interference.

Eta(z)/kappa：测量纵向弛豫干扰的横向弛豫优化光谱核磁共振实验。