A Multi-functional Optical Impedance Microscope for Live Cell Imaging

用于活细胞成像的多功能光阻抗显微镜

基本信息

批准号：
8072748
负责人：
SHAOPENG WANG
金额：
$ 17.65万
依托单位：
ARIZONA STATE UNIVERSITY-TEMPE CAMPUS
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-05-15 至 2013-02-28
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8072748
关键词：
Address Algorithms Behavior Biological Biological Assay Biological Models Biomedical Research Cell Adhesion Cell Culture Techniques Cell Extracts Cell Proliferation Cell surface Cell-Cell Adhesion Cell-Matrix Junction Cells Cellular Morphology Charge Chemicals Clinical Confocal Microscopy Dependence Development Electric Capacitance Electrodes Event Film Flow Cytometry Fluorescence Fluorescence Microscopy Glass Goals Gold Image Image Analysis Imaging Techniques In Situ Individual Label Lateral Life Light Maps Measurement Measures Mechanics Methodology Methods Microfabrication Microscope Microscopy Modeling Monitor Noise Optics Phase Phase-Contrast Microscopy Process Research Personnel Resolution Sampling Shunt Device Signal Transduction Slide Spectrum Analysis Substrate Interaction Surface Surface Plasmon Resonance System Techniques Technology Testing Time Toxicology Work Wound Healing angiogenesis base cell behavior cell growth cell motility cellular imaging data acquisition density electric impedance image processing imaging modality innovation instrument interest novel public health relevance research study sensor submicron success tool voltage

项目摘要

DESCRIPTION (provided by applicant): Project Summary Electrical impedance spectroscopy (EIS) is a sensitive label-free technique that has proved to be a powerful tool for a wide range of live cell studies. Measurement of the local impedance of live cells on an entire surface is highly desired, but so far has not been possible with current EIS technology. This project aims at the development of a new microscopy that can capture high-resolution impedance images of live cells. The proposed new microscopy is based on principles that are completely different from the conventional EIS. Instead of measuring impedance electrically, it images the local impedance of the entire surface optically with sub-micron spatial resolution. This simplifies the impedance measurement without sacrificing sensitivity and, more importantly, it introduces new exciting capabilities including: 1) sensor chips can be easily fabricated and prepared for cell attachment; 2) the entire sensor chip or selected region of interest can be analyzed for detailed studies, which is important because it enables the tracking of individual cells or even region within single cells with the best sensitivity and spatial resolution; 3) conventional surface plasmon resonance images can be obtained simultaneously, which provide detailed information on cell/substrate interaction; and 4) the instrument will be built based on a conventional inverted optical microscope, so that in-situ phase contrast and fluorescence microscopy images can be obtained for the same sample if desired. The project includes the following four tasks: 1) build a high-resolution optical impedance microscope system; 2) establish data acquisition, processing, and analysis algorithms for live cell analysis; 3) study the relationships between impedance microscopy images and cell adhesion behavior; and 4) test and evaluate the optical impedance microscope for additional studies of cells including wound healing, toxicology and motility. PUBLIC HEALTH RELEVANCE (provided by applicant): This project aims at the development of a new label-free microscopy that can capture sub-micron resolution impedance images of live cells optically. In addition, conventional surface plasmon resonance, optical and fluorescence microscopy images can be obtained simultaneously. The success of this project will provide a new tool that has a broad range of applications on cell dynamic studies.

描述（由申请人提供）：项目摘要电阻抗光谱 (EIS) 是一种灵敏的无标记技术，已被证明是广泛活细胞研究的强大工具。人们非常希望测量整个表面上活细胞的局部阻抗，但目前的 EIS 技术还无法实现。该项目旨在开发一种新型显微镜，可以捕获活细胞的高分辨率阻抗图像。所提出的新型显微镜基于与传统 EIS 完全不同的原理。它不是以电气方式测量阻抗，而是以亚微米空间分辨率对整个表面的局部阻抗进行光学成像。这在不牺牲灵敏度的情况下简化了阻抗测量，更重要的是，它引入了令人兴奋的新功能，包括：1）可以轻松制造和准备传感器芯片以用于细胞附着； 2）可以分析整个传感器芯片或选定的感兴趣区域以进行详细研究，这很重要，因为它能够以最佳灵敏度和空间分辨率跟踪单个细胞甚至单个细胞内的区域； 3) 可同时获得常规表面等离子体共振图像，提供细胞/基质相互作用的详细信息； 4）该仪器将基于传统的倒置光学显微镜构建，以便在需要时可以获得同一样品的原位相差和荧光显微镜图像。该项目包括以下四项任务：1）构建高分辨率光阻抗显微镜系统； 2）建立活细胞分析的数据采集、处理和分析算法； 3）研究阻抗显微图像与细胞粘附行为的关系； 4) 测试和评估光阻抗显微镜，以进行细胞的其他研究，包括伤口愈合、毒理学和运动性。公共健康相关性（由申请人提供）：该项目旨在开发一种新型无标记显微镜，可以光学捕获活细胞的亚微米分辨率阻抗图像。此外，还可以同时获得常规的表面等离子体共振、光学和荧光显微镜图像。该项目的成功将为细胞动态研究提供一种具有广泛应用的新工具。

项目成果

期刊论文数量（8）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Plasmonic-based imaging of local square wave voltammetry.

DOI：
10.1021/ac201392r
发表时间：
2011-10-01
期刊：
ANALYTICAL CHEMISTRY
影响因子：
7.4
作者：
Shan, Xiaonan;Wang, Shaopeng;Wang, Wei;Tao, Nongjian
通讯作者：
Tao, Nongjian

Single cells and intracellular processes studied by a plasmonic-based electrochemical impedance microscopy.

DOI：
10.1038/nchem.961
发表时间：
2011-03
期刊：
NATURE CHEMISTRY
影响因子：
21.8
作者：
Wang, Wei;Foley, Kyle;Shan, Xiaonan;Wang, Shaopeng;Eaton, Seron;Nagaraj, Vinay J.;Wiktor, Peter;Patel, Urmez;Tao, Nongjian
通讯作者：
Tao, Nongjian

Label-free measuring and mapping of binding kinetics of membrane proteins in single living cells.