IRON K-BETA XES STUDIES OF THE NITROGENASE ENZYME SYSTEM AND MODELS

固氮酶系统和模型的铁 K-β XES 研究

• 批准号: 8169942
• 负责人: SERENA D DE BEER GEORGE
• 金额: $ 2.31万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8169942
• 关键词: Biological Models; Carbon; Complex; Computer Retrieval of Information on Scientific Projects Database; Data; Data Analyses; Enzymes; Funding; Grant; Institution; Investigation; Iron; Ligands; MYO5A gene; Methods; Modeling; Molybdoferredoxin; Nitrogen; Nitrogenase; Oxygen; Research; Research Personnel; Resources; Series; Source; Structure; System; United States National Institutes of Health; interest; mutant; oxidation

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. K-beta XES data have been obtained on a series of Fe(II) and Fe(III) model complexes in order to develop an understanding of the changes which occur in the Kbeta1,3 (3p-1s) and Kbeta2,5 (valences to 1s) emission spectra as a function of spin state, oxidation state and local structure. Of particular interest are the Kbeta? crossover features which are empirically correlated to a ligand 2s to 1s transition, and as such may be used to differentiate carbon from nitrogen from oxygen. Analyses of these data are currently underway. In addition, preliminary data on the MoFe protein of nitrogenase and the delta-nifB mutant have been obtained and demonstrate the feasibility of these methods for investigation of dilute protein systems.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 K-Beta XES数据已在一系列的Fe（II）和Fe（III）模型复合物上获得，以便对KBETA1,3（3P-1S）和KBETA2,5（1S）发射光谱的变化有所了解，这是自旋状态，氧化状态和局部结构的功能。特别感兴趣的是KBETA吗？在经验上与配体2s至1s转变相关的交叉特征，因此可以用来将碳与氮与氧气区分开。这些数据的分析目前正在进行中。此外，已经获得了有关氮基酶MOFE蛋白和Delta-NIFB突变体的初步数据，并证明了这些方法用于研究稀蛋白系统的可行性。