Biophysical Response Factors to Weak Electric and Magnetic Fields

对弱电场和磁场的生物物理响应因素

基本信息

批准号：
7894802
负责人：
Mark Thomas Hernandez
金额：
$ 35.34万
依托单位：
UNIVERSITY OF COLORADO
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-07-15 至 2012-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7894802
关键词：
Acute Address Affect Air Antibiotic Resistance Archives Arts Automobile Driving Bacillus subtilis Bacteria Bacterial Model Biochemical Biological Biological Assay Biomedical Engineering Cancerous Cataloging Catalogs Cell Culture Techniques Cell Line Cell Wall Cell physiology Cells Chemicals Cytosol DNA Damage Devices Digital Signal Processing Disinfection Dose Electromagnetic Fields Electromagnetics Engineering Escherichia coli Eukaryotic Cell Exposure to Fibroblasts Focal Infection Frequencies Future Gene Expression Gene Expression Profile Generations Growth Harvest Heating Image Immobilized Cells Immune In Situ Investigation Ionic Strengths Ionizing radiation Ions Laser Surgery Lasers Lead Leukocytes Liquid substance Literature Magnetic Resonance Imaging Malignant Neoplasms Mammalian Cell Maps Mechanical Stress Membrane Potentials Microscopic Microscopy Modeling Molecular Molecular Profiling Monitor Operative Surgical Procedures Operon Optics Outcome Oxidation-Reduction Pathway interactions Permeability Physiological Plasmids Publishing Relative (related person)Reporter Reporting Reproduction spores Research Site Small Interfering RNA Staging Stress Study models Surface System Techniques Temperature Testing Therapeutic Thermometry Time Tissues Water absorption analog base biological adaptation to stress biological systems cell killing cell motility cohort density design electric field energy density engineering design infectious disease treatment magnetic field membrane assembly microbial microorganism mutant neutrophil novel operation research study response sound stressor voltage

项目摘要

Different electric and magnetic field (E/MF) configurations have been applied for non-invasive imaging therapies and surgery (laser) for many years. Unlike their higher energy counterparts, the biological mechanisms underlying the effects of exposure to low-power E/MF remain largely uncharacterized while therapeutic applications are beginning to emerge. Some cellular research themes have emerged in the last decade to help bridge the understanding of fundamental cellular responses to weak E/MFs, into therapeutic pathways. In this context, the salient theme driving this proposal parse into two main themes with respect to weak E/MF exposure: (i) inactivation of cells, and (ii) sub-lethal doses that activate or otherwise affect different cellular functions. Our objective is to establish dose-response thresholds for bacterial and eukaryotic cells on a on a fundamental biophysical level, which maps into understanding (and controlling) organized cellular-system response in weak E/MF fields. The central hypothesis driving these investigations is: that the effects of E/MF on bacterial and eukaryotic cells have common mechanistic bases. We believe we can elucidate fundamental biological responses to E/MF exposures by leveraging the respective strengths of widely-accepted cell culture practices with modern biochemical and transcriptome analyses. We have engineered bench-scale experimental apparati to isolate and combine electric and magnetic fields of various strengths; these systems permit real-time, direct microscopic access to cells as they are suspended in electromagnetic fields. We can thus observe intra- and intercellular E/MF responses (both physical and chemical) in real time, and establish threshold values for lethal and sub-lethal EM doses. We will investigate the effect of AC, DC and (isolated) magnetic field exposure utilizing the strengths of these experimental systems. We will juxtapose our cellular-level findings to leukocyte motility models, and investigate how mechanistic observations can be applied to systems with therapeutic benefits. Relevance: A mechanistic understanding of the effects weak E/MF have on biological systems will aid in the design, engineering and operations of therapeutic devices that currently utilize E/MF, or may do so in the near future.

多年来，不同的电场和磁场 (E/MF) 配置已应用于非侵入性成像治疗和手术（激光）。与高能量对应物不同，低功率 E/MF 影响的生物机制在很大程度上仍未得到表征，而治疗应用已开始出现。过去十年中出现了一些细胞研究主题，以帮助将对弱 E/MF 的基本细胞反应的理解与治疗途径联系起来。在这种情况下，推动该提案的突出主题解析为关于弱 E/MF 暴露的两个主题：(i) 细胞失活，以及 (ii) 激活或以其他方式影响不同细胞功能的亚致死剂量。我们的目标是在基本的生物物理水平上建立细菌和真核细胞的剂量反应阈值，这映射到理解（和控制）弱 E/MF 场中组织的细胞系统反应。推动这些研究的中心假设是：E/MF 对细菌和真核细胞的影响具有共同的机制基础。我们相信，通过利用广泛接受的细胞培养实践的各自优势以及现代生化和转录组分析，我们可以阐明对 E/MF 暴露的基本生物学反应。我们设计了小型实验装置来隔离和组合不同强度的电场和磁场；这些系统允许实时、直接地在显微镜下观察悬浮在电磁场中的细胞。因此，我们可以实时观察细胞内和细胞间的 E/MF 反应（物理和化学），并建立致死和亚致死 EM 剂量的阈值。我们将利用这些实验系统的优势来研究交流、直流和（隔离）磁场暴露的影响。我们将把我们的细胞水平发现与白细胞运动模型并列，并研究如何将机械观察应用于具有治疗益处的系统。相关性：从机制上理解弱 E/MF 对生物系统的影响将有助于目前或在不久的将来利用 E/MF 的治疗设备的设计、工程和操作。

项目成果

期刊论文数量（4）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Reduction of the background magnetic field inhibits ability of Drosophila melanogaster to survive ionizing radiation.

DOI：
10.1002/bem.21720
发表时间：
2012-12
期刊：
BIOELECTROMAGNETICS
影响因子：
1.9
作者：
Portelli, Lucas A.;Madapatha, Dinu R.;Martino, Carlos;Hernandez, Mark;Barnes, Frank S.
通讯作者：
Barnes, Frank S.

LacI(Ts)-regulated expression as an in situ intracellular biomolecular thermometer.

LacI(Ts) 调节表达作为原位细胞内生物分子温度计。