Scientific Core

科学核心

• 批准号: 7918235
• 负责人: JUDAH FOLKMAN
• 金额: $ 42.72万
• 依托单位: BOSTON CHILDREN'S HOSPITAL
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7918235
• 关键词: Angiogenesis Promoter; Biological Assay; Biology; Blood Vessels; Cells; Chick Embryo; Confocal Microscopy; Cornea; Embryo; Endothelial Cells; Goals; Housing; Image; In Vitro; Laboratory Assistant; Lasers; Life; Measures; Microscope; Modeling; Mus; Photography; Publications; Regulation; Research; Research Personnel; Scanning; Services; Slide; Testing; Time; Tissues; angiogenesis; cell preparation; chorioallantoic membrane; digital; experience; in vivo; inhibitor/antagonist; member; programs; reconstruction; tumor

The goal of the Scientific Core is to support and facilitate the research programs of the investigators in the program project. The overall Scientific Core consists of a Photography Sub-Core, an Angiogenesis Assay Sub-Core, and an Imaging Sub-Core, (i) The Photography Core provides high quality, in-house conventional and digital services, including figures for publication, and images for slide and PowerPoint presentations. This Core is staffed by one full-time photographer with over 10 years experience in the department (ii) The Angiogenesis Assay Core will provide microvascular endothelial cells and expertise in carrying out angiogenesis assays, including chick aortic rings to measure angiogenesis in vitro, the mouse cornea assay and chick chorioallantoic membrane assay to test angiogenesis promoters and inhibitors in vivo. This Core is staffed by one senior technician (with over 29 years experience) and a laboratory assistant. The Core does large and small vessel endothelial cell preparation and biology, and develops the chick embryo Angiogenesis model, (iii) The Imaging Core will provide a centralized facility for laser-confocal microscopy, real-time imaging, and three-dimensional reconstruction of living cells, whole embryos, and histological sections of tumors and developing vascular tissue. The Imaging Core will be staffed by an experienced confocal microscopist who will operate the shared laser-scanning confocal microscope, instruct and assist Project members in its use, and manage access to the facility. This new facility is critical to a number of the specific aims of the Program Project.

科学核心的目的是支持和促进研究人员的研究计划 程序项目。总体科学核心由摄影子核组成，一种血管生成 测定子核和成像子核，（i）摄影核心提供高质量的内部 常规和数字服务，包括出版物的数字以及幻灯片和PowerPoint的图像 演讲。该核心由一位全职摄影师组成，拥有超过10年的经验 部门（ii）血管生成测定核心将提供微血管内皮细胞和专业知识 在进行血管生成测定时，包括雏鸡主动脉环在体外测量血管生成， 小鼠角膜测定和鸡绒毛膜膜测定法测试血管生成启动子和 体内抑制剂。该核心由一位高级技术人员（拥有超过29年的经验）和 实验室助理。核心进行大和小血管内皮细胞的制备和生物学， 并开发雏鸡胚胎血管生成模型，（iii）成像核将提供集中式 激光焦点显微镜，实时成像和三维生活的设施 细胞，整个胚胎和肿瘤的组织学切片和发展血管组织。成像 核心将由经验丰富的共聚焦显微镜组成，他们将经营共享的激光扫描 共聚焦显微镜，指导和协助项目成员使用并管理对设施的访问。 这个新设施对于计划项目的许多特定目标至关重要。