Genetics and neurobiology of aggression of Betta splendens

芨芨草攻击行为的遗传学和神经生物学

• 批准号: 10731186
• 负责人: Pei Yin Shih
• 金额: $ 12.5万
• 依托单位: COLUMBIA UNIV NEW YORK MORNINGSIDE
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-08-15 至 2025-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10731186
• 关键词: 3D Print; Affect; Aggressive behavior; Amygdaloid structure; Animal Behavior; Animals; Behavior; Behavioral; Brain region; Breeding; CRISPR/Cas technology; Cessation of life; Chromosome Mapping; Classification; Code; Custom; Data; Development; Development Plans; Economic Burden; Etiology; Excitatory Synapse; Faculty; Fishes; Foundations; Frequencies; Generations; Genes; Genetic; Genetic Variation; Genomics; Gills; Goals; Heritability; Human; Image; Individual; Individual Differences; Infrastructure; Inhibitory Synapse; Knock-out; Knowledge; Lung; Machine Learning; Maintenance; Maps; Mediating; Mentorship; Methods; Mind; Modeling; Molds; Molecular; Mollies; Mus; Nervous System; Neurobiology; Neurons; Partner in relationship; Pathway interactions; Pattern; Performance; Phase; Physiological; Population Genetics; Positioning Attribute; Quantitative Trait Loci; Recording of previous events; Research; Research Activity; Research Personnel; Resource Development; Resources; Robot; Ruta; Shapes; Signal Transduction; Social Behavior; Societies; Specific qualifier value; Statistical Methods; Synapses; System; Techniques; Technology; Testing; Tracer; Transgenic Organisms; Variant; Work; autism spectrum disorder; base; behavior test; behavioral response; brain behavior; career; career development; erection; falls; fighting; gain of function; gene discovery; genetic approach; genetic variant; innovation; insight; interdisciplinary approach; interest; neural; neural circuit; neuroligin 3; neuronal circuitry; neuropsychiatry; novel; novel strategies; overexpression; siamese fighting fish; skills; tool; trait; visual stimulus

Project Summary / Abstract Project description: Aggression is a competitive social behavior that animals use to acquire resources and protect kin. While genetic variation is known to contribute to individual differences in aggression, we know very little about the specific genes involved and their mechanisms. To discover genes that regulate aggression, the applicant will exploit the unique features of the Siamese fighting fish Betta splendens (betta). Highly aggressive fighting betta have been generated through a long history of artificial breeding. Their aggressive behaviors are easily triggered by visual stimuli, and their intense fighting continues until retreat or death. The applicant proposes to use these fighting fish, along with their unselected, less aggressive wild counterparts, to expand our knowledge of genetic and neuronal bases underlying aggression. In Aim 1 (K99), the applicant will characterize the behavioral differences between wild and fighting betta during fights by developing a novel behavioral test and utilizing machine-learning based tools for behavioral analyses. In Aim 2 (K99), the applicant will discover aggression-associated variants that contribute to the behavioral differences with complementary mapping methods and genetic tools for functional perturbation. Finally, in Aim 3 (R00), the applicant will probe how the mapped genes influence the nervous system and mediate aggression using neuronal imaging and circuit tracing tools. Candidate and career goals: The applicant’s broad research interests involve understanding how genetic variation shapes the nervous system to modulate behaviors. The proposed research activities will build upon the applicant’s strong background in genetics and genomics skillsets with imaging and circuit tracing techniques, enabling the interdisciplinary approach necessary to tackle the applicant’s highly innovative research questions. The proposed project will also lay the foundation for the applicant’s long-term goal as an independent researcher who brings computational, genetic, and neurobiological fields together to create bold new approaches for understanding social behavior. Career development plan: The K99 phase of this work will be done in Columbia’s Zuckerman Mind Brain Behavior Institute (ZMBBI) under the mentorship of Drs. Andrés Bendesky and Franck Polleux. In addition, the candidate has assembled an extraordinary team of advisors and collaborators, consisting of Drs. Molly Przeworski, Carolyn (Lindy) McBride, Vanessa Ruta, and David Manuel Flores Gonçalves. The applicant’s research and transition to independence will benefit from this strong mentorship team, the state-of-the-art facilities and infrastructure at ZMBBI, and numerous Professional Development resources offered through ZMBBI and Columbia.

项目概要/摘要 项目描述：攻击是动物为了获取资源和利益而产生的一种竞争性社会行为。 保护亲人 虽然众所周知，遗传变异会导致攻击性的个体差异，但我们非常清楚。 为了发现调节攻击性的基因，人们对所涉及的特定基因及其机制知之甚少。 申请人将利用暹罗斗鱼Betta splendens（斗鱼）的独特特征，具有高度攻击性。 斗鱼是经过长期的人工饲养而产生的，它们的攻击行为是。 很容易被视觉刺激触发，他们激烈的战斗会一直持续到撤退或死亡。 建议利用这些斗鱼以及它们未经选择的、攻击性较小的野生人员来扩大 我们对攻击行为背后的遗传和神经基础的了解 在目标 1 (K99) 中，申请人将 通过编写小说来描述野生斗鱼和格斗斗鱼在打斗过程中的行为差异 行为测试并利用基于机器学习的工具进行行为分析。 申请人将发现与攻击相关的变异，这些变异导致了行为差异 最后，在目标 3 (R00) 中，功能扰动的互补绘图方法和遗传工具。 申请人将探究绘制的基因如何影响神经系统并使用 神经成像和电路追踪工具。 候选人和职业目标：申请人广泛的研究兴趣涉及了解遗传如何 拟议的研究活动将建立在变异塑造神经系统的基础上。 申请人在遗传学和基因组学技能方面具有强大的背景，包括成像和电路追踪 技术，从而能够采用必要的跨学科方法来解决申请人的高度创新 研究问题也将为申请人的长期目标奠定基础。 独立研究人员将计算、遗传和神经生物学领域结合在一起，创造出大胆的 理解社会行为的新方法。 职业发展计划：K99阶段的这项工作将在哥伦比亚大学的Zuckerman Mind Brain完成 行为研究所 (ZMBBI) 在安德烈斯·本德斯基 (Andrés Bendesky) 和弗兰克·波勒 (Franck Polleux) 博士的指导下。 候选人组建了一支由顾问和合作者组成的非凡团队，其中包括莫莉博士。 Przeworski、Carolyn (Lindy) McBride、Vanessa Ruta 和 David Manuel Flores Gonçalves 申请人。 研究和向独立的过渡将受益于这个强大的指导团队、最先进的 ZMBBI 的设施和基础设施，以及通过提供的众多专业发展资源 ZMBBI 和哥伦比亚。