The KAPP-Sen Tissue Mapping Center Collaborative

KAPP-森组织绘图中心合作

基本信息

批准号：
10376627
负责人：
VESNA D GAROVIC
金额：
$ 270万
依托单位：
UNIVERSITY OF CONNECTICUT SCH OF MED/DNT
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-09-30 至 2026-08-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10376627
关键词：
Address Adipose tissue Age Aging Atlases Bioinformatics Biological Biological Aging Biological Markers Biology Biometry Biopsy Boston Brain Death Cell Aging Cesarean section Chronic Disease Clinic Clinical Clinical Trials Collaborations Complex Cytometry Data Data Analyses Dermatology Diabetes Mellitus Discipline of obstetrics Donor person Elderly Elements Endocrinology Evaluation Fatty acid glycerol esters Future Generations Genomic medicine Genomics Geriatrics Geroscience Goals Gynecology Health Heart Heterogeneity Hospitals Human Image Individual Institution Kidney Kidney Transplantation Knowledge Laboratories Nephrology Outpatients Pancreas Pathology Phase Phenotype Placenta Positioning Attribute Pregnancy Program Evaluation RLK5-associated protein phosphatase Research Research Activity Research Design Research Personnel Resources Risk Factors Sample Size Sampling Site Skin Skin Tissue Spatial Distribution Specific qualifier value Syndrome Thick Tissues Translations United States National Institutes of Health Woman design flexibility human subject human tissue innovation insight interest modifiable risk multidisciplinary racial and ethnic sample collection scale up senescence sex single cell technology skills telomere transcriptomics translational study

项目摘要

PROJECT SUMMARY OVERALL This proposal seeks to establish the KAPP-Sen Tissue Mapping Center Collaborative (TMC) as part of the Cellular Senescence Network: Tissue Mapping Centers effort {RFA-RM-21-008). Our application represents a multidisciplinary collaborative effort involving six leading institutions and aging research programs working together to characterize the distribution and biological heterogeneity of senescent cells in different healthy human tissues in full alignment with the objectives of the Cellular Senescence Network {SenNet). KAPP-Sen brings together skills, resources and perspectives needed to address Sen-Net goals in the framework of the healthy human Kidney. Adipose tissues, Pancreas and Placenta. Given our ability to obtain full thickness skin tissues from individuals providing KAPP samples, we may explore the possibility of future collaborative studies with TMCs selected for their major focus on skin. Our six collaborating institutions located in Farmington. CT (UConn Health, Jackson Laboratory for Genomic Medicine), Boston {Brigham & Women's Hospital, Joslin Diabetes Center), Rochester. MN {Mayo Clinic) and San Antonio. TX {UTHCSA) have all been carefully selected for their essential and unique individual contributions to the field and this effort. Our objectives will be achieved through the following aims: Aim 1 : Coordinate research activities across KAPP-Sen TMC Collaborative sites in support of Sen-Net goals towards mapping cellular senescence and its associated secretory phenotype in the healthy human kidney, adipose tissues, pancreas and placenta. Aim 2: Obtain tissues from healthy kidney transplant donors {kidneys, fat, skin), C-section pregnancies {placenta, cord, fat, skin), outpatient healthy donor biopsies {fat, skin), beating heart brain dead donors {full pancreas) and IIPD/Prodo {dispersed pancreas). Aim 3: Generate highest quality data pertaining to cellular senescence including scRNA-Seq, snRNA-Seq, spatial transcriptomics, immunohistochemistry and Telomere-associated DDR foci {TAFs) in all tissues Aim 4: Perform high level integrative data analysis required for the creation of aUases of human cellular senescence in collaboration with other TMCs, CODDC and NIH staff.

项目总体概要该提案旨在建立 KAPP-Sen 组织绘图中心协作组织 (TMC)，作为细胞衰老网络：组织绘图中心工作 (RFA-RM-21-008) 的一部分。我们的应用代表了多学科的合作努力，涉及六个领先机构和衰老研究项目，共同努力描述不同健康人体组织中衰老细胞的分布和生物异质性，与细胞衰老网络（SenNet）的目标完全一致。 KAPP-Sen 汇集了在健康人类肾脏框架内实现 Sen-Net 目标所需的技能、资源和观点。脂肪组织、胰腺和胎盘。鉴于我们能够从提供 KAPP 样本的个人那里获得全层皮肤组织，我们可能会探索未来与主要关注皮肤的 TMC 进行合作研究的可能性。我们的六家合作机构位于法明顿。 CT（康涅狄格大学健康中心、杰克逊基因组医学实验室）、波士顿（布莱根妇女医院、乔斯林糖尿病中心）、罗彻斯特。明尼苏达州（梅奥诊所）和圣安东尼奥。 TX (UTHCSA) 均因其对该领域和这项工作的重要且独特的个人贡献而经过精心挑选。我们的目标将通过以下目标来实现：目标 1：协调 KAPP-Sen TMC 协作站点的研究活动，以支持 Sen-Net 的目标，即绘制健康人肾脏、脂肪组织、胰腺和胎盘中的细胞衰老及其相关的分泌表型。目标 2：从健康肾移植供体（肾脏、脂肪、皮肤）、剖腹产妊娠（胎盘、脐带、脂肪、皮肤）、门诊健康供体活检（脂肪、皮肤）、心脑死亡供体（全胰腺）获取组织）和 IIPD/Prodo（分散胰腺）。目标 3：生成与细胞衰老相关的最高质量数据，包括所有组织中的 scRNA-Seq、snRNA-Seq、空间转录组学、免疫组织化学和端粒相关 DDR 病灶 (TAF) 目标 4：与其他 TMC、CODDC 和 NIH 工作人员合作，执行创建人类细胞衰老疾病所需的高水平综合数据分析。