The synergistic effects of climate and anthropogenic drivers on toxic cyanobacterial blooms

气候和人为驱动因素对有毒蓝藻水华的协同影响

• 批准号: 9789304
• 负责人: George S Bullerjahn
• 金额: $ 12.5万
• 依托单位: BOWLING GREEN STATE UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9789304
• 关键词: Address; Affect; Agriculture; Algae; Algal Blooms; Biological Availability; Biomass; Budgets; Characteristics; Chemicals; Climate; Communities; Cues; Cyanobacterium; Data; Data Set; Development; Ecology; Environmental Health; Event; Exposure to; Fresh Water; Goals; Growth; Habitats; Health; Human; Metagenomics; Microbe; Microcystis; Molecular Biology; Natural Resources; Nature; Nitrates; Nitrogen; Nutrient; Organism; Pathway interactions; Physiology; Play; Population; Production; Research Priority; Research Project Grants; Risk; Role; Severities; Shapes; Source; Structure; Surveys; System; Systems Biology; Techniques; Temperature; Testing; Toxic effect; Toxin; Urea; Ursidae Family; Variant; Water; Water Supply; Work; anthropogenesis; climate change; cyanobacterial toxin; data management; data resource; design; environmental chemistry; experimental study; harmful algal blooms; hepatotoxin; human disease; interdisciplinary approach; member; metabolomics; metatranscriptomics; microbial community; microbiome; microbiota; microcystin; success; tool; transcriptomics; trigger point; waterborne

Project Summary Cyanobacterial bloom events are shaped by environmental cues that contribute to harmful algal bloom (cHAB) composition and toxigenicity. Indeed, in Lake Erie, two distinctly different cyanobacterial genera, Microcystis and Planktothrix, produce microcystin, a potent hepatotoxin. Whereas both cyanobacteria bloom in waters affected by nutrients delivered by agricultural runoff, Microcystis typically blooms in offshore waters, and Planktothrix dominates nearshore embayments. Prior work has suggested a role of climate-change induced temperature increases in promoting growth of Microcystis, and that Planktothrix persists in waters affected by prolonged nitrogen depletion. Given the different characteristics of these two organisms, limiting exposure to microcystin may require different management strategies to protect the water supply. Our overarching aim in this project is to address the extent to which N availability and temperature play in the development, toxigenicity and persistence of cHAB taxa in Lake Erie and other bloom-affected freshwaters. Informed by baseline data from metatranscriptomic surveys, we will design microcosm and chemostat experiments aimed at understanding competition between the two Lake Erie cHAB genera and factors contributing to bloom toxicity. Specifically, we will test three hypotheses in this project listed below: 1) Nitrogen availability controls cHAB community structure. Microcosms of mixed Microcystis and Planktothrix bloom communities will be exposed to different regimes of N availability and temperature to determine the degree to which each contribute to the dominance of one bloom-former over another. 2) Microcystin production is dependent on temperature and N speciation. Chemostat studies of Microcystis and Planktothrix cultures at control and warmer temperature will reveal how different chemical forms of N (nitrate vs. urea) promote growth and toxin production. 3) The proliferation of cHAB species is promoted by the activity of the limnetic microbiome. Metatransciptomic analysis of bloom communities will provide an assessment of the activities of the entire microbial consortium in cHAB events in order to determine what organisms and pathways assist in cHAB development, persistence and decline. A second aim of this project is to generate a comprehensive Lake Erie environmental `omics data set that can be shared and utilized by the integrated research projects within the Great Lakes Center for Fresh Waters and Human Health.

项目概要 蓝藻水华事件是由导致有害藻华 (cHAB) 的环境因素决定的 成分和毒性。事实上，在伊利湖中存在着两个截然不同的蓝藻属，即微囊藻属（Microcystis）。 和浮丝菌，产生微囊藻毒素，一种强效肝毒素。而两种蓝藻都在水中开花 受农业径流输送的养分影响，微囊藻通常在近海水域繁殖，并且 浮丝菌在近岸海湾占主导地位。先前的工作表明气候变化引起的作用 温度升高促进微囊藻的生长，并且浮游丝菌在受影响的水域中持续存在 长期缺氮。鉴于这两种生物体的不同特征，限制接触 微囊藻毒素可能需要不同的管理策略来保护供水。我们的总体目标是 该项目旨在解决氮的可用性和温度在开发中发挥作用的程度， 伊利湖和其他受水华影响的淡水中 cHAB 类群的毒性和持久性。消息灵通 根据宏转录组调查的基线数据，我们将设计微观世界和恒化器实验，旨在 了解伊利湖两个 cHAB 属之间的竞争以及导致水华毒性的因素。 具体来说，我们将在该项目中测试以下三个假设： 1) 氮的可用性控制 cHAB 群落结构。混合微囊藻的微观世界和 浮丝菌水华群落将暴露于不同的氮利用率和温度条件下 确定每种开花因子对一种开花因子相对于另一种开花因子的主导作用的程度。 2) 微囊藻毒素的产生取决于温度和氮形态。微囊藻的恒化器研究 和浮游丝菌在控制和较高温度下的培养将揭示不同的化学形式如何 N（硝酸盐与尿素）促进生长和毒素产生。 3) 湖沼微生物组的活性促进了 cHAB 物种的增殖。 对水华群落的元转录组学分析将提供对整个水华群落活动的评估 cHAB 事件中的微生物联盟，以确定哪些生物体和途径有助于 cHAB 发展、持续和衰退。 该项目的第二个目标是生成一个全面的伊利湖环境组学数据集，可以 由五大湖淡水中心内的综合研究项目共享和利用 人类健康。