A GENERIC METHOD TO STUDY BACTERIOPHAGE/HOST INTERACTIONS

研究噬菌体/宿主相互作用的通用方法

• 批准号: 8169128
• 负责人: Seth A. Darst
• 金额: $ 0.12万
• 依托单位: ROCKEFELLER UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-03-01 至 2011-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8169128
• 关键词: Affinity; Bacterial RNA; Bacteriophage T4; Bacteriophages; Cells; Computer Retrieval of Information on Scientific Projects Database; DNA-Directed RNA Polymerase; Escherichia coli; Funding; Generic Drugs; Grant; Institution; Manuscripts; Methodology; Methods; Modification; Procedures; Proteome; Publishing; Research; Research Personnel; Resources; Route; Source; United States National Institutes of Health

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Bacteriophages are bacterial viruses that infect bacterial cells, and they have developed ingenious mechanisms to modify the bacterial RNA polymerase. Using a rapid, specific, single-step affinity isolation procedure to purify Escherichia coli RNA polymerase from bacteriophage T4-infected cells, we have identified bacteriophage T4-dependent modifications of the host RNA polymerase. We suggest that this methodology is broadly applicable for the identification of bacteriophage-dependent alterations of the host synthesis machinery. A manuscript describing this method was published: Rapid isolation and identification of bacteriophage T4-encoded modifications of Escherichia coli RNA polymerase: a generic method to study bacteriophage/host interactions. Westblade LF, Minakhin L, Kuznedelov K, Tackett AJ, Chang EJ, Mooney RA, Vvedenskaya I, Wang QJ, Feny¿ D, Rout MP, Landick R, Chait BT, Severinov K, Darst SA. J Proteome Res. 2008; 7(3):1244-50.

该副本是使用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这是调查员的机构。 噬菌体是感染细菌细胞的细菌病毒，它们已经开发出巧妙的机制来修饰细菌RNA聚合酶。使用快速，特定的单步亲和分离程序从细菌T4感染的细胞中纯化大肠杆菌RNA聚合酶，我们已经鉴定出对宿主RNA聚合酶的细菌T4依赖性修饰。我们建议该方法广泛适用于鉴定宿主合成机制的噬菌体依赖性改变。描述此方法的手稿已发表： 快速分离和鉴定大肠杆菌RNA聚合酶的细菌噬细胞T4代码修饰：一种研究菌株/宿主相互作用的通用方法。 Westblade LF，Minakhin L，Kuznedelov K，Tackett AJ，Chang EJ，Mooney RA，Vvedenskaya I，Wang QJ，Feny¿D，Rout MP，Rout MP，Landick R，Chait BT，Chait BT，Severinov K，Severinov K，Darst SA。 J蛋白质组res。 2008; 7（3）：1244-50。