Burkholderia pseudomallei autotransporters and TPS proteins

鼻疽伯克霍尔德氏菌自转运蛋白和 TPS 蛋白

• 批准号: 8069265
• 负责人: David Alan Low
• 金额: $ 34.39万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2014-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8069265
• 关键词: Actins; Adherence; Bordetella bronchiseptica; Bordetella pertussis; Burkholderia pseudomallei; C-terminal; Cell Line; Cell surface; Centers for Disease Control and Prevention (U.S.); Characteristics; Cytoplasm; Environment; Epithelial Cells; Escherichia coli; Eukaryotic Cell; Family member; Genes; Genome; Goals; Gonorrhea; Gram-Negative Bacteria; Growth; Haemophilus influenzae; Heme; Human; IgA-specific serine endopeptidase; Mediating; Membrane; N-terminal; Names; Pathogenesis; Pathway interactions; Peptide Signal Sequences; Protein Secretion; Proteins; Proteolysis; Role; Serine Protease; Structure; System; Yersinia; cytotoxicity; extracellular; in vivo; pertactin; polymerization; protein expression; uptake

Autotransporters (ATs) compose a large superfamily of proteins produced by Gram-negative bacteria (47). They consist of an N-terminal signal sequence, a "passenger domain" that mediates the protein's effector function, and a C-terminal "p-domain" involved in translocation of the passenger domain across the outer membrane. "Classical" ATs are monomeric with (3-domains of ~26o aa, while trimeric ATs have (3-domains of -70 aa, three of which form the outer membrane p-barrel structure (47). Classical ATs include the IgA protease ofNeisseria gonorrhoeae, IcsA of Shigellaflexneri, pertactin of Bordetella pertussis, and the serine protease autotransporters of enterpbacteriaceae (SPATEs) (69). Trimeric ATs include YadA of Yersinia species and Hia of Haemophilus influenzae, both of which are involved in adherence (69). Two Partner Secretion (TPS) systems, also widespread among Gram-negative bacteria, are conceptually similar to ATs, except the passenger and p-domains are synthesized as separate proteins known generically as the TpsA and TpsB family members, respectively (104). The TpsA "exoprotein" often is synthesized with a prodomain at either the N- or C-terminus that is removed during translocation, and while some TpsA proteins are released into the extracellular environment, others remain anchored to the cell surface (66,105) ((122,133). Predicting the presence of a pro-domain, whether the mature protein will be released, and, if not, its topology on the cell surface currently is not possible. Functions associated with TpsA proteins include adherence, cytotolysis, heme uptake, and contact-dependent growth inhibition (69). The B. pseudomallei K96243 genome (71) contains 10 putative AT-encoding genes and one TPSencoding locus. One AT, the BPSS1492 or bimA gene product, induces actin polymerization, allowing B. pseudomallei to move within eukaryotic cells' cytoplasm (138). Neither the other AT-encoding OPvFs, nor the genes encoding the putative TPS system, which we named btpA and btpB, have been studied.

自转运蛋白（AT）由革兰氏阴性细菌产生的一个大的蛋白质超家族组成 (47)。它们由 N 端信号序列、介导蛋白质效应子的“过客结构域”组成 功能，以及涉及乘客结构域跨外层易位的 C 端“p 结构域” 膜。 “经典”AT 是单体，具有（约 26o 个氨基酸的 3 个结构域），而三聚体 AT 具有（约 26 个氨基酸的 3 个结构域） -70 个氨基酸，其中三个形成外膜 p 桶结构 (47)。经典 AT 包括 IgA 蛋白酶 淋病奈瑟氏球菌、福氏志贺氏菌 IcsA、百日咳博德特氏菌百日咳杆菌百日咳杆菌粘附素和丝氨酸蛋白酶 肠杆菌科自转运蛋白（SPATE）（69）。三聚体 AT 包括耶尔森菌属的 YadA 和 Hia 流感嗜血杆菌，两者都与依从性有关 (69)。 两个伙伴分泌 (TPS) 系统在革兰氏阴性细菌中也广泛存在，从概念上讲 与 AT 类似，不同之处在于乘客结构域和 p 结构域是作为单独的蛋白质合成的，通常称为 分别是 TpsA 和 TpsB 家族成员 (104)。 TpsA“外蛋白”通常与前结构域一起合成 在易位过程中被移除的 N 或 C 末端，而一些 TpsA 蛋白 释放到细胞外环境中，其他部分仍然锚定在细胞表面 (66,105) ((122,133)。 预测前结构域的存在、成熟蛋白是否会被释放，如果不会，则预测其拓扑结构 目前在细胞表面是不可能的。与 TpsA 蛋白相关的功能包括粘附、 细胞溶解、血红素摄取和接触依赖性生长抑制 (69)。 B.伪鼻疽 K96243 基因组 (71) 包含 10 个假定的 AT 编码基因和 1 个 TPSencoding 轨迹。一种 AT（BPSS1492 或 bimA 基因产物）可诱导肌动蛋白聚合，从而使 B. 假鼻疽在真核细胞的细胞质内移动 (138)。其他 AT 编码 OPvF 和 编码假定的 TPS 系统（我们将其命名为 btpA 和 btpB）的基因已被研究。