Morphology Core

形态核心

基本信息

批准号：
7647668
负责人：
THOMAS A DRAKE
金额：
$ 23.8万
依托单位：
UNIVERSITY OF CALIFORNIA LOS ANGELES
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-04-01 至 2014-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7647668
关键词：
Aneurysm Antibodies Aorta Aortic Aneurysm Arachidonate 5-Lipoxygenase Aromatase Arterial Fatty Streak Atherosclerosis Brain CCL2 gene Cell Adhesion Molecules Cells Cholesterol Cleaved cell Confocal Microscopy Core Facility Cultured Cells Detection Development Differentiation Antigens Elastin Electron Microscopy Endothelial Cells Engineering Equipment Face Fluorescent Probes Freeze Etching Frozen Sections Gelatinase B Gene Expression Genes Genetically Engineered Mouse Grant Heart Hematoxylin Histocytochemistry Histopathology Human Imagery Immune Immunohistochemistry Inflammation Knock-out Label Lesion Lesion by Morphology Light Microscope Lipids Lipoidosis Lipoproteins Lung Macrophage Inflammatory Protein-1 Mentors Metabolism Methodology Microglia Microscopic Microtomy Morphology Mus Pathologist Peroxidases Plant Roots Postdoctoral Fellow Procedures Process Proteins Publishing Reporter Genes Sampling Series Services Smooth Muscle Smooth Muscle Myocytes Staining method Stains Structure Structure of brachiocephalic artery Students System Techniques Testing Tissues Training Trichrome stain method alveolar lamellar body animal tissue arteriole cellular imaging cost cryostat cytochemistry cytokine digital imaging experience human tissue image processing in vivo macrophage member oil red O oxidized lipid pneumocyte programs research study

项目摘要

The Morphology Core will provide comprehensive morphologic procedures and analyses for the 6 component Projects. All 6 component Projects will use .genetically defined and/or engineered mice that will require morphologic analyses. Thus, a core facility is needed to provide the highest quality, most efficient, and cost-effective morphologic services. By far, the most heavily used service of this Gore has been and will, be quantitative analyses of atherosclerotic lesions in the aorta and the innominate arteries of mice. However, a broad range of morphologic procedures are required by the 6 projects that include immunolabeling of tissues, labeling and analysis of cultured cells and isolated cells with fluorescent, probes for confocal microscopy, and histopathologic assessment of the aorta and innominate arteries, and in some cases other tissues, for the effects of gene manipulation in mice.

形态核心将提供全面的形态学程序和分析6 组件项目。所有6个组件项目将使用。需要形态学分析。因此，需要一个核心设施来提供最高质量，最高效的设施和具有成本效益的形态服务。到目前为止，这种血腥的最常用的服务已经并且将对主动脉和小鼠无知动脉的动脉粥样硬化病变进行定量分析。但是，包括免疫标签的6个项目需要广泛的形态学程序组织，培养细胞和荧光细胞分离的细胞的标记和分析探针共焦显微镜以及主动脉和无知动脉的组织病理学评估，在某些情况下案例其他组织，导致基因操纵在小鼠中的影响。