CHARACTERIZATION OF NAG EXPRESSION AND FUNCTIONS

NAG 表达和功能的表征

• 批准号: 7960001
• 负责人: CARLA J GUTHRIDGE
• 金额: $ 11.95万
• 依托单位: UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-05-01 至 2010-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7960001
• 关键词: Affect; Anti-Inflammatory Agents; Anti-inflammatory; Autoimmune Diseases; Biomedical Research; Caenorhabditis elegans; Cell physiology; Cells; Characteristics; Computer Retrieval of Information on Scientific Projects Database; Development; Disease; Funding; Gene Proteins; Genes; Grant; Homologous Gene; Inflammation; Inflammatory; Institution; MYCN gene; Malignant Neoplasms; Mediating; Molecular; Neuroblastoma; Oklahoma; Patients; Pattern; Play; Process; Protein Isoforms; Proteins; Research; Research Personnel; Resources; Role; Source; System; Tissues; United States National Institutes of Health; anakinra; cell type; interest; mRNA Decay; outcome forecast; tumorigenesis

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The focus of this proposal is to understand the molecular and cellular functions of neuroblastoma amplified gene product (NAG), an uncharacterized protein with interesting associations to tumorigenesis, inflammation, and development. The NAG gene is co-amplified with MYCN in neuroblastoma cells; however its expression has been correlated with better prognosis in patients with neuroblastoma. NAG also has been shown to interact with interleukin-1 receptor antagonist, a known anti-inflammatory molecule. In C. elegans NAG expression is important for normal development and has been shown to play a role in nonsense-mediated mRNA decay. By understanding how NAG acts to regulate cellular processes, an understanding of how its expression can affect disease processes such as those associated with cancer and inflammatory autoimmune diseases will be appreciated. Our basic hypothesis is that NAG exists as multiple forms which differentially impact cellular processes and development. To understand the impact of these forms on cellular processes and development, we propose to characterize the expression of NAG isoforms in multiple cell types. We anticipate that different patterns of NAG isoforms will be expressed in these different cell types and that the NAG isoforms will have shared and unique functions important for cellular processes characteristic of the tissue system from which those cells are derived. Additionally, we will develop a C. elegans system to study the NAG homologue. We anticipate the NAG homologue expressed in C. elegans will share basic functions analogous to the vertebrate NAG protein.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 该建议的重点是了解神经母细胞瘤扩增基因产物（NAG）的分子和细胞功能，这是一种未表征的蛋白质，与肿瘤发生，炎症和发育有有趣的关联。 NAG基因与神经母细胞瘤细胞中的MYCN共扩散。然而，它的表达与神经母细胞瘤患者的预后更好。 NAG还已显示与白介素-1受体拮抗剂（一种已知的抗炎分子）相互作用。 在秀丽隐杆线虫中，NAG表达对于正常发育很重要，并且已显示在胡说八道介导的mRNA衰减中起作用。 通过了解NAG如何调节细胞过程，将对其表达如何影响疾病过程，例如与癌症和炎症自身免疫性疾病相关的疾病过程。 我们的基本假设是，NAG作为多种形式存在，这些形式会差异地影响细胞过程和发育。 为了了解这些形式对细胞过程和发育的影响，我们建议表征多种细胞类型中NAG同工型的表达。 我们预计，不同的NAG同工型模式将在这些不同的细胞类型中表达，并且NAG同工型将具有共享和独特的功能，对于从中得出这些细胞的组织系统的特征的细胞过程很重要。 此外，我们将开发一种秀丽隐杆线虫系统来研究NAG同源物。 我们预计秀丽隐杆线虫中表达的NAG同源物将共享类似于脊椎动物NAG蛋白的基本功能。