STRESS AND THE CETACEAN HEART

压力与鲸类心脏

• 批准号: 7960092
• 负责人: ZACHARY A PFIEFFER
• 金额: $ 3.55万
• 依托单位: UNIVERSITY OF ALASKA FAIRBANKS
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-05-01 至 2010-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7960092
• 关键词: Address; Affect; Alaska; Anatomy; Animals; Cardiac conduction system; Catecholamines; Cells; Computer Retrieval of Information on Scientific Projects Database; Disease; Electron Microscopy; Environmental Pollution; Funding; Grant; Health; Heart; Histology; Injury; Institution; Laboratories; Link; Mammals; Marines; Microscopic; Necrosis; Organ; Research; Research Personnel; Resources; Source; Stress; Structure; System; Techniques; Tissue Preservation; Tissues; Transmission Electron Microscopy; United States National Institutes of Health; Work; environmental agent; interest

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Overview: In a broad sense, my laboratory is interested in identifying and understanding factors that affect the health of marine mammals. Much of our recent research efforts have focused on the effects of stress on the health and function of cetaceans. Factoring centrally into this work is an interest in the potential link between environmental contaminants and stress in cetaceans. The work proposed here will help define how stress may affect particular organs within an animal and thus it represents an important initial step in efforts to address the link between contaminants, stress, and cetacean health. Specific Objectives: 1. Characterize the normal histological features of the conduction system of the heart in T. truncatus. 2. Examine the conduction system in stranded T. truncatus for evidence of catecholamine-induced injury such as contraction band necrosis. General Approach: Standard techniques will be followed for correlating the gross anatomy with the microscopic topography of intracardiac structures. Next, histology and when tissue preservation is sufficient, transmission electron microscopy, will be used to characterize the cellular features of the cardiac conduction system. Finally, we will use histology and when feasible, electron microscopy, to probe for evidence of cell/tissue changes consistent with catecholamine-induced injury. These degenerative changes have been well characterized in various species (Haft, 1974; Windingstad et al., 1983; Turnbull and Cowan, 1998).

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 概述： 从广义上讲，我的实验室有兴趣确定和理解影响海洋哺乳动物健康的因素。我们最近的许多研究工作都集中在压力对鲸类健康功能的影响上。集中考虑这项工作是对环境污染物与鲸类压力之间潜在联系的兴趣。这里提出的工作将有助于定义压力如何影响动物内部的特定器官，因此，它代表了解决污染物，压力和鲸类健康之间联系的重要第一步。 特定目标： 1。表征T. truncatus中心脏传导系统的正常组织学特征。 2。检查滞留的T. truncatus中的传导系统，以了解儿茶酚胺诱导的损伤的证据，例如收缩带坏死。 一般方法： 将遵循标准技术，将总的解剖结构与心脏内结构的显微镜形貌相关联。接下来，组织学和组织保存足够时，透射电子显微镜将用于表征心脏传导系统的细胞特征。最后，我们将使用组织学并在可行的电子显微镜时探测与儿茶酚胺诱导的损伤一致的细胞/组织变化的证据。这些退化性变化在各种物种中都得到了很好的特征（Haft，1974； Windingstad等，1983； Turnbull和Cowan，1998）。