GENE REGULATION DURING COMPENSATORY NEURONAL REGENERATION

代偿性神经元再生过程中的基因调控

• 批准号: 7960065
• 负责人: HADLEY W HORCH
• 金额: $ 6.55万
• 依托单位: MOUNT DESERT ISLAND BIOLOGICAL LAB
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-05-23 至 2010-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7960065
• 关键词: Auditory; Axon; Clinical; Computer Retrieval of Information on Scientific Projects Database; Dendrites; Denervation; Development; Ear; Excision; Funding; Gene Expression Regulation; Goals; Grant; Growth; Gryllidae; Injury; Institution; Interneurons; Invertebrates; Investigation; Lead; Maine; Natural regeneration; Neuronal Injury; Neurons; Research; Research Personnel; Resources; Source; Synapses; System; United States National Institutes of Health; Work; axon regeneration; comparative; functional genomics; loss of function; neuronal circuitry; reinnervation; response; synaptogenesis

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Neuronal injuries induce profound changes in axons, dendrites, and synapses that usually lead to a devastating loss of function. While a large amount of research has broadened our understanding of axonal regeneration, very little is known about the ability of dendrites to regenerate after injury or denervation. Obviously, any successful clinical strategy will eventually need to consider the regeneration of dendrites and synapses if the full complexity of neuronal circuitry is to be restored. Thus, it is of great importance to understand dendritic regeneration and to identify factors that may be involved. The long-term goal of my research is to elucidate the factors which influence dendritic growth and plasticity. Specifically, work in my lab examines the compensatory regeneration of auditory interneurons in the cricket. Past research has demonstrated that unilateral removal of the ear in crickets induces denervated interneuron dendrites to grow across the midline, a boundary they usually observe, and form functional synaptic connections with the auditory afferents from the opposite ear (Hoy et al., 1985; Schildberger et al., 1986). This reinnervation is remarkably precise, reinstating interneuron-specific threshold and intensity responses. The central hypothesis of my research is that the compensatory regeneration and synapse formation of auditory dendrites in the cricket is guided by a recapitulation of the expression of developmental molecules. It is my hope that an investigation of this invertebrate regeneration phenomenon will advance our fundamental understanding of the plasticity of dendrites, and reveal principles governing dendritic regeneration that may be applicable to other neuronal systems.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 神经元损伤会导致轴突，树突和突触的深刻变化，这些变化通常导致功能丧失。 尽管大量研究扩大了我们对轴突再生的理解，但对于树突在受伤或神经支配后的再生能力知之甚少。 显然，如果要恢复神经元电路的全部复杂性，任何成功的临床策略最终都需要考虑树突和突触的再生。 因此，了解树突状再生并确定可能涉及的因素非常重要。 我的研究的长期目标是阐明影响树突状生长和可塑性的因素。 具体来说，我的实验室工作检查了板球中听觉中间神经元的补偿性再生。 过去的研究表明，板球中的耳朵的单侧去除会诱导被剥夺的interneuron树突在整个中线上生长，它们通常观察到一个边界，并形成功能性突触连接与相反耳朵的听觉传入（Hoy等，1985； Schildberger等； Schildberger等； Schildberger等，1986）。 这种重新连接非常精确，可以恢复间含有特定的阈值和强度响应。 我的研究的核心假设是，板球中听觉树突的补偿性再生和突触形成是通过对发育分子表达的概括而指导的。我希望对这种无脊椎动物再生现象的调查能够提高我们对树突可塑性的基本理解，并揭示有关树突再生的原则，这些原则可能适用于其他神经元系统。