STRUCTURE OF THE FCRN RECEPTOR

FCRN 受体的结构

• 批准号: 7955051
• 负责人: MARK S LADINSKY
• 金额: $ 1.07万
• 依托单位: UNIVERSITY OF COLORADO
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-08-01 至 2010-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7955051
• 关键词: Adherent Culture; Apical; Binding; Blood; Cell surface; Cells; Cellular Structures; Computer Retrieval of Information on Scientific Projects Database; Epithelium; Fc Immunoglobulins; Food; Freeze Substitution; Freezing; Funding; Gold; Grant; Immunoglobulin G; Institution; MDCK cell; Neonatal; Preparation; Process; Rattus; Research; Research Personnel; Resources; Sampling; Side; Source; Structure; Tissues; Tomogram; United States National Institutes of Health; Vesicle; nanoGold; neonatal Fc receptor; particle; receptor; tomography

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The neonatal Fc receptor (FcRn) transports immunoglobulin G (IgG) across epithelia, binding IgG into acidic vesicles (pH< 6.5) that form on the apical surface of the cell and releasing IgG into the blood at pH7.4. Structural features of this process are being studied by EM tomography. In particular we have conjugated nanogold particles to the constant fragment (Fc) of IgG and have both administered the conjugates at low pH to cultured monolayers of MDCK cells and given them as food to neonatal rats. Following preparation of the relevant cells or tissues by rapid freezing and freeze substitution, we have looked for evidence of gold internalization in the vesicular compartments at and near the apical side of the cells. Preliminary tomograms of these samples show some internalization of gold, however it is difficult to visualize.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 新生儿FC受体（FCRN）将免疫球蛋白G（IgG）横穿上皮细胞，将IgG结合到酸性囊泡中（pH <6.5），在细胞的顶部表面形成，并将IgG释放到pH7.4时的血液中。 EM断层扫描研究了此过程的结构特征。 特别是，我们将纳米颗粒共轭到IgG的恒定片段（FC），并在低pH值下给MDCK细胞培养的单层偶联物施用，并将其作为新生大鼠的食物。 通过快速冷冻和冻结取代制备相关细胞或组织后，我们在细胞顶端的顶端及其附近寻找了金属内化的证据。 这些样品的初步断层图显示了黄金的一些内在化，但是很难可视化。