PROTEOMIC METHOD FOR IDENTIFYING MEMBRANE PROTEINS

鉴定膜蛋白的蛋白质组学方法

• 批准号: 7955062
• 负责人: MARK S LADINSKY
• 金额: $ 1.07万
• 依托单位: UNIVERSITY OF COLORADO
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-08-01 至 2010-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7955062
• 关键词: Cell physiology; Cellular Structures; Computer Retrieval of Information on Scientific Projects Database; Funding; Grant; Institution; Integral Membrane Protein; Journals; Membrane; Membrane Proteins; Methods; Nature; Pharmacologic Substance; Preparation; Procedures; Proteome; Proteomics; Research; Research Personnel; Resources; Shotguns; Source; Staging; Transmembrane Domain; United States National Institutes of Health; Work; electron tomography; high throughput analysis; reconstruction; three dimensional structure

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Integral membrane proteins perform crucial cellular functions and are the targets for the majority of pharmaceutical agents. However, the hydrophobic nature of their membrane-embedded domains makes them difficult to work with. Here, we describe a shotgun proteomic method for the high-throughput analysis of the membrane-embedded transmembrane domains of integral membrane proteins which extends the depth of coverage of the membrane proteome. Electron tomography and 3D reconstruction is used to deduce the 3D structure of the membrane preparations at different stages of the procedures. This work is currently in press in the Journal of Proteome Research.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 整体膜蛋白具有至关重要的细胞功能，是大多数药物的靶标。 但是，其膜包裹的域的疏水性使它们很难使用。 在这里，我们描述了一种shot弹枪蛋白质组学方法，用于对整体膜蛋白的膜包裹的跨膜结构域进行高通量分析，该膜蛋白扩展了膜蛋白质组覆盖深度。 电子断层扫描和3D重建用于在过程的不同阶段推断膜制剂的3D结构。 目前，这项工作在《蛋白质组研究杂志》杂志上。