WORKSHOP FOR TRAINING MICROSCOPE ADMINISTRATORS IN THE SETUP AND USE OF SERIALEM

培训显微镜管理员设置和使用 Serialem 的研讨会

• 批准号: 7955050
• 负责人: DAVID N MASTRONARDE
• 金额: $ 2.14万
• 依托单位: UNIVERSITY OF COLORADO
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-08-01 至 2010-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7955050
• 关键词: Administrator; Calibration; Cellular Structures; Computer Retrieval of Information on Scientific Projects Database; Dose; Educational workshop; Electronics; Evaluation; Feedback; Funding; Grant; Hand; Health Sciences; Institutes; Institution; Learning; Medicine; Microscope; New York; Paper; Participant; Persons; Research; Research Personnel; Resources; Series; Source; Texas; Time; Training; United States National Institutes of Health; Universities; college; design; digital; electron tomography; experience; improved; lectures; reconstruction; structural biology; tomography; tool

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This year, we added an additional workshop that targeted microscope administrators for the setup and use of SerialEM. The workshop was held December 6-8, 2006 where we hosted 6 participants from the following institutions: University of Calgary, Baylor College of Medicine, University of Texas Health Science Center, New York Structural Biology Center, Brandeis University, and The Weizmann Institute. The number of participants was kept deliberately small mainly to give each participant enough time to really learn how to use SerialEM on the microscopes. In addition, we could only put 3 additional people in each microscope room comfortably. The workshop was designed to have a morning lecture and an afternoon with intensive hands-on experience. The first day focused on introducing SerialEM and setting up for automated tilt-series acquisition. The second day focused on low-dose applicationsincluding cryo-electron tomography, energy filtering, and the Navigator. Finally, the third day focused on using macros, calibration of SerialEM, and digital cameras. Each person received the lectures and handouts in both electronic and paper format so they could use those tools train their microscope facility users on how to use SerialEM. All participants gave favorable evaluations. In addition, valuable feedback was obtained from the participants on how to improve SerialEM. Three participants received extra training; one for high magnification tomography, and two for reconstruction of cryo tilt-series.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 今年，我们添加了一个其他研讨会，该研讨会针对了显微镜管理员，以设置和使用SeriaLem。该研讨会于2006年12月6日至8日举行，我们接待了以下机构的6名参与者：卡尔加里大学，贝勒医学院，德克萨斯大学健康科学中心，纽约结构生物学中心，布兰代斯大学和魏兹曼学院。参与者的数量是故意的，主要是为每个参与者提供足够的时间来真正学习如何在显微镜上使用Serialem。此外，我们只能在每个显微镜室里舒适地将3个额外的人放在。该研讨会的目的是进行早晨的演讲和一个下午，并具有丰富的动手经验。第一天的重点是引入Serialem并设置自动倾斜系列的收购。第二天的重点是低剂量应用，包括冷冻电子断层扫描，能量过滤和导航器。最后，第三天的重点是使用宏，Serialem的校准和数码相机。每个人都以电子和纸张格式收到讲座和讲义，因此他们可以使用这些工具训练其显微镜设施使用者如何使用SeriaLem。所有参与者都进行了有利的评估。此外，从参与者那里获得了有关如何改善Serialem的宝贵反馈。三名参与者接受了额外的培训；一个用于高放大层扫描术，另一幅用于重建冷冻倾斜系列。