Experimental Mice

实验小鼠

基本信息

批准号：
7944527
负责人：
THOMAS DOETSCHMAN
金额：
$ 19.69万
依托单位：
UNIVERSITY OF ARIZONA
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-08-19 至 2014-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7944527
关键词：
American Animal Housing Animal Welfare Animal Welfare Act Animals Arizona Arts Biometry Blood specimen Breathing Breeding Cancer Center Cancer Center Support Grant Cancer cell line Carbon Dioxide Cells Certification Chemopreventive Agent Clinical Trials Committee Members Communities Cryopreservation Data Collection Development Diet Embryo Ensure Euthanasia Experimental Designs Fatty acid glycerol esters Funding Gases Gene Targeting Genetic Engineering Genetically Engineered Mouse Growth Health Sciences Hour Housing Human Resources IACUC Injection of therapeutic agent Knock-out Laboratory Animals Laboratory Research Life Mails Maintenance Malignant Neoplasms Mammary gland Medical Methods Modeling Mouse Strains Mus Mycoplasma Oral Performance Policies Procedures Production Protocols documentation Public Health Puncture procedure Quality Control Recommendation Regulation Reporting Research Research Personnel SCID Mice Scientist Screening procedure Services Specialist System Tail Technical Expertise Techniques Technology Therapeutic Training Transfection Transgenic Mice Transgenic Organisms Universities Update Veins Veterinarians Work Writing Xenograft procedure animal care animal facility authority comparative computerized cost design design and construction embryonic stem cell experience human cancer mouse model in vivo lectures member mouse model pathogen programs research facility research study square foot vector

项目摘要

9.1.3 EXPERIMENTAL MOUSE SHARED SERVICE The Experimental Mouse Shared Service (EMSS) is a one-stop shared service for all your in vivo mouse experimentation needs. The purpose of the EMSS is to provide investigators at the Arizona Cancer Center (AZCC) with experimental mouse models and with a wide range of mouse experimentation techniques. The EMSS has combined the previous Genetically Engineered Mouse "Developmental" Shared Service (GEMSS) and the previous EMSS. Therefore, the EMSS begins with the design and construction of gene targeting and transgenic mouse vectors, gene targeting and ES cell screening for targeted Clones, production of genetargeted mice, production of transgenic mice, screening for transgenic and knockout founders, embryo rederivation for pathogen cleanup and cryopreservation of mouse strains through to complete experimentation services in all aspects of xenograft and GEM models. The specific objectives of the EMSS include creation of models, maintenance of shared and investigator colonies, assistance in the design of in vivo experiments, and performance of mouse experiments by personnel with expertise in mouse experimental protocols. Hence, the EMSS provides consistency and quality of procedures within and between mouse experiments that ensures accuracy in experimental results, confidence in interpretation of results, and an inner-consistency between mouse experiments which is essential for intra-lab and inter-lab comparative analyses. Due to the fact that the mouse is a model for all translational cancer work for both NIH-type funding as well as progression to clinical trials, the EMSS is an absolutely essential shared service. The benefits of the EMSS are to provide AZCC investigators with a cost-effective mechanism for the creation of different mouse models and for completing in vivo mouse experiments utilizing highly trained and experienced technicians. The EMSS continues to expand its services in a very cost-effective and efficient manner.

9.1.3实验鼠标共享服务实验鼠标共享服务（EMSS）是用于所有体内鼠标的一站式共享服务实验需求。 EMS的目的是在亚利桑那癌症中心提供调查员（AZCC）具有实验小鼠模型和各种小鼠实验技术。这 EMS已将以前的基因工程鼠标“发育”共享服务（GEMSS）结合在一起和以前的EMS。因此，EMS始于基因靶向的设计和构建和转基因小鼠载体，基因靶向和ES细胞筛选靶向克隆，产生基因靶标小鼠，转基因小鼠的产生，筛查转基因和敲除创始人，胚胎通过病原体清理和对小鼠菌株冷冻保存的重新培训，以完成实验异种移植和宝石模型的各个方面的服务。 EMS的具体目标包括创建模型，共享和研究者殖民地的维护，体内实验设计的帮助以及通过具有小鼠实验方案专业知识的人员进行小鼠实验的表现。因此， EMS提供了鼠标实验内和之间的过程的一致性和质量，以确保实验结果的准确性，对结果解释的信心以及内部矛盾小鼠实验对于LAB内和LAB间比较分析至关重要。由于事实小鼠是所有转化癌的模型，用于NIH型资金以及临床的发展试验，EMSS是绝对必要的共享服务。 EMS的好处是提供AZCC 研究人员具有成本效益的机制，用于创建不同的鼠标模型，并完成使用经验丰富且经验丰富的技术人员进行了体内小鼠实验。 EMS继续扩展它的服务以非常有效和高效的方式。