Experimental Mice

实验小鼠

基本信息

批准号：
7944527
负责人：
THOMAS DOETSCHMAN
金额：
$ 19.69万
依托单位：
UNIVERSITY OF ARIZONA
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-08-19 至 2014-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7944527
关键词：
American Animal Housing Animal Welfare Animal Welfare Act Animals Arizona Arts Biometry Blood specimen Breathing Breeding Cancer Center Cancer Center Support Grant Cancer cell line Carbon Dioxide Cells Certification Chemopreventive Agent Clinical Trials Committee Members Communities Cryopreservation Data Collection Development Diet Embryo Ensure Euthanasia Experimental Designs Fatty acid glycerol esters Funding Gases Gene Targeting Genetic Engineering Genetically Engineered Mouse Growth Health Sciences Hour Housing Human Resources IACUC Injection of therapeutic agent Knock-out Laboratory Animals Laboratory Research Life Mails Maintenance Malignant Neoplasms Mammary gland Medical Methods Modeling Mouse Strains Mus Mycoplasma Oral Performance Policies Procedures Production Protocols documentation Public Health Puncture procedure Quality Control Recommendation Regulation Reporting Research Research Personnel SCID Mice Scientist Screening procedure Services Specialist System Tail Technical Expertise Techniques Technology Therapeutic Training Transfection Transgenic Mice Transgenic Organisms Universities Update Veins Veterinarians Work Writing Xenograft procedure animal care animal facility authority comparative computerized cost design design and construction embryonic stem cell experience human cancer mouse model in vivo lectures member mouse model pathogen programs research facility research study square foot vector

项目摘要

9.1.3 EXPERIMENTAL MOUSE SHARED SERVICE The Experimental Mouse Shared Service (EMSS) is a one-stop shared service for all your in vivo mouse experimentation needs. The purpose of the EMSS is to provide investigators at the Arizona Cancer Center (AZCC) with experimental mouse models and with a wide range of mouse experimentation techniques. The EMSS has combined the previous Genetically Engineered Mouse "Developmental" Shared Service (GEMSS) and the previous EMSS. Therefore, the EMSS begins with the design and construction of gene targeting and transgenic mouse vectors, gene targeting and ES cell screening for targeted Clones, production of genetargeted mice, production of transgenic mice, screening for transgenic and knockout founders, embryo rederivation for pathogen cleanup and cryopreservation of mouse strains through to complete experimentation services in all aspects of xenograft and GEM models. The specific objectives of the EMSS include creation of models, maintenance of shared and investigator colonies, assistance in the design of in vivo experiments, and performance of mouse experiments by personnel with expertise in mouse experimental protocols. Hence, the EMSS provides consistency and quality of procedures within and between mouse experiments that ensures accuracy in experimental results, confidence in interpretation of results, and an inner-consistency between mouse experiments which is essential for intra-lab and inter-lab comparative analyses. Due to the fact that the mouse is a model for all translational cancer work for both NIH-type funding as well as progression to clinical trials, the EMSS is an absolutely essential shared service. The benefits of the EMSS are to provide AZCC investigators with a cost-effective mechanism for the creation of different mouse models and for completing in vivo mouse experiments utilizing highly trained and experienced technicians. The EMSS continues to expand its services in a very cost-effective and efficient manner.

9.1.3 实验鼠标共享服务实验鼠标共享服务（EMSS）是为您所有体内鼠标提供的一站式共享服务实验需要。 EMSS 的目的是为亚利桑那州癌症中心的研究人员提供 (AZCC) 拥有实验小鼠模型和广泛的小鼠实验技术。这 EMSS结合了之前的基因工程小鼠“发育”共享服务（GEMSS）和之前的EMSS。因此，EMSS从基因打靶的设计和构建开始转基因小鼠载体、基因靶向和 ES 细胞筛选靶向克隆、基因靶向的生产小鼠、转基因小鼠的生产、转基因和基因敲除创始人的筛选、胚胎重新衍生病原体清除和小鼠品系冷冻保存直至完成实验异种移植和 GEM 模型各个方面的服务。 EMSS 的具体目标包括创建模型、共享和研究者菌落的维护、体内实验设计的协助，以及由具有小鼠实验方案专业知识的人员进行小鼠实验。因此， EMSS 提供小鼠实验内部和之间程序的一致性和质量，确保实验结果的准确性、结果解释的可信度以及之间的内在一致性小鼠实验对于实验室内和实验室间的比较分析至关重要。由于以下事实：小鼠是所有转化癌症工作的模型，无论是 NIH 类型的资助还是临床进展试验表明，EMSS是绝对必要的共享服务。 EMSS的好处是提供AZCC 研究人员具有成本效益的机制来创建不同的小鼠模型并完成体内小鼠实验由训练有素且经验丰富的技术人员进行。 EMSS持续扩大其服务非常具有成本效益和高效。