PROJECT 3 : QUANTITATIVE BIOMARKERS OF DNA AND PROTEIN OXIDATION

项目 3：DNA 和蛋白质氧化的定量生物标志物

• 批准号: 7977068
• 负责人: Paul Thomas Henderson
• 金额: $ 31.99万
• 依托单位: UNIVERSITY OF CALIF-LAWRNC LVRMR NAT LAB
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-04 至 2010-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7977068
• 关键词: Amino Acids; Animals; Arteriosclerosis; Biological; Biological Markers; Cells; Computer Retrieval of Information on Scientific Projects Database; DNA; DNA biosynthesis; Diabetes Mellitus; Disease; Dose; Funding; Goals; Grant; Institution; Isotope Labeling; Label; Leukocytes; Malignant Neoplasms; Metabolic; Methods; Modification; Nucleosides; Nucleotides; Oxidative Stress; Pathway interactions; Peroxidases; Proteins; RNA; Research; Research Personnel; Resource Development; Resources; Source; United States National Institutes of Health; accelerator mass spectrometry; age related; chlorination; human disease; oxidation; protein metabolite

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Project 3 is developing methods to quantify oxidative modifications in DNA, proteins, and metabolites. We will focus on introducing 14C isotope labels into biological molecules in order to characterize pathways and biomarkers of relevance to human disease. This labeling is done by either dosing cells or animals with a labeled building block such as an amino acid for incorporation into proteins or a nucleoside for synthesis of DNA. Two main objectives of Project 3 are: (1) characterization of nucleotide pool oxidation products that are incorporated into DNA and RNA and (2) chlorination of proteins by HOCl produced by myeloperoxidase during the activation of white blood cells. Completion of these objectives will enable progress towards the general goal of quantifying the metabolic flux of molecules produced as a result of oxidative stress which may be related to cancer, diabetes, arteriosclerosis and numerous aging-related diseases.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 项目3正在开发定量DNA，蛋白质和代谢物中氧化修饰的方法。 我们将重点介绍将14C同位素标记引入生物分子中，以表征与人类疾病相关的途径和生物标志物。 该标记是通过用剂量细胞或具有标记的构件的动物来完成的，例如氨基酸，用于掺入蛋白质或用于合成DNA的核苷。 项目3的两个主要目标是：（1）将核苷酸池氧化产物的表征掺入DNA和RNA中，以及（2）在白细胞激活期间由骨髓氧化酶产生的HOCL氯化蛋白质。 这些目标的完成将使朝着量化可能与癌症，糖尿病，动脉粥样硬化和许多与衰老有关的疾病有关的氧化应激产生的分子的代谢通量的总体目标实现。