SBIR TOPIC 257, INSTRUMENTS AND DEVICES THAT PRESERVE MOLECULAR PROFILES IN TUMO

SBIR 主题 257，保留 TUMO 中分子特征的仪器和设备

• 批准号: 7962681
• 负责人: MARK JOHNSON
• 金额: $ 24.96万
• 依托单位: PHOENIX ANALYSIS AND DESIGN TECHNOLOGIES
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-30 至 2010-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7962681
• 关键词: SBIR; TOPIC; 257; INSTRUMENTS; DEVICES

Biopsies play an essential role in developing and monitoring of personalized cancer treatments. Current biopsy methods for visceral tumors are ineffective at preserving the molecular profile of the tumor due to the rapid enzymatic reactions that take place during and after the procedure. Our approach is to develop an automated biopsy device that (1) Limits molecular profile degradation by rapidly preserving the biopspecimen, (2) ensures process consistency by automating the steps to obtain and preserve the tumor sample, and (3) provides biomarker assessment flexibility by allowing users to choose cryogenic or chemical preservation. This proposal describes a device concept that utilizes interchangeable cartridges that automatically store and preserve the biopsy sample using either cryogenic or chemical fixative. In this project, we will design and fabricate a prototype device and evaluate its preservation effectiveness by testing it on Rats and SCID mice with a pancreatic cancer cell line model. We plan to use immunohistochemistry, pathology, proteomics, and genomics for evaluating how well the device preserves the molecular profile of the tumor tissue. When successfully completed, the findings of this Phase-1 work will demonstrate the feasibility of our approach and provide the Design Inputs for further developing the device for clinical use.

活检在个性化癌症治疗的开发和监测中发挥着重要作用。当前的 内脏肿瘤的活检方法无法有效保留肿瘤的分子特征，因为 手术期间和手术后发生的快速酶反应。我们的方法是开发一个 自动活检装置 (1) 通过快速保留分子谱来限制分子谱退化 生物样本，(2) 通过自动化获取和保存肿瘤的步骤来确保流程的一致性 样本，(3) 通过允许用户选择低温或化学来提供生物标志物评估灵活性 保存。该提案描述了一种利用可互换墨盒的设备概念， 使用低温或化学固定剂自动存储和保存活检样本。在这个 项目中，我们将设计并制造原型装置，并通过测试评估其保存效果 它对具有胰腺癌细胞系模型的大鼠和 SCID 小鼠进行了实验。我们计划使用免疫组织化学， 病理学、蛋白质组学和基因组学，用于评估设备保留分子特征的程度 肿瘤组织。成功完成后，第一阶段工作的结果将证明 我们的方法的可行性，并为进一步开发临床使用的设备提供设计输入。