HIGH FAT DIET INDUCED ALTERATIONS IN GENE EXPRESSION IN THE NONHUMAN PRIMATE

高脂肪饮食引起非人类灵长类动物基因表达的改变

• 批准号: 7958879
• 负责人: JOSEPH E ROBERTSON
• 金额: $ 49.85万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-28 至 2010-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7958879
• 关键词: Adult; Animals; Brain; Child; Clinical; Clinical Research; Communities; Computer Retrieval of Information on Scientific Projects Database; Coronary heart disease; Data; Databases; Development; Diet; Disease; Eating; Environment; Fatty Liver; Fatty acid glycerol esters; Funding; Future; Gene Expression; Gene Proteins; Genomics; Grant; Health; Human; Hypothalamic structure; Institution; Liver diseases; Metabolic; Metabolic Diseases; Modeling; Non-Insulin-Dependent Diabetes Mellitus; Obesity; Obesity associated disease; Overweight; Pathogenesis; Phenotype; Physiological; Population; Prevalence; Proteomics; Research; Research Personnel; Resources; Rodent; Rodent Model; Scientist; Source; Study Subject; System; Therapeutic Intervention; Translations; United States; United States National Institutes of Health; hypertensive heart disease; insight; nonhuman primate; novel; protein expression; research study; therapeutic development

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): Obesity is a worldwide health epidennic and a major contributor to the increased occurrence of coronary heart disease, hypertension, fatty liver disease, and type 2 diabetes. During the past 30 years, the prevalence of overweight and obesity has increased for both adults and children in the United States. About 60 million adults, or 30% of the adult population, are now obese. It is well accepted that many factors contribute to the development of obesity and associated disorders. Both clinical research and studies in rodent models have provided key insights into the pathogenesis of obesity and associated diseases, as well as their impact on the brain. However, human and rodent studies are subject to limitations that can hamper their direct translation to effective therapeutic interventions. The nonhuman primate (NHP) has emerged as a critical model for integrating data from rodent and human studies. The overall objective of this proposal is to perform parallel physiological, genomic, and proteomic analyses in a NHP model of high-fat diet-induced obesity (DIO). The major strengths of this model are that: 1) The progression of DiO in the NHP is similar to that in humans, and this model develops the full spectrum of associated diseases; 2) the experimental environment of the animals is carefully controlled so that food intake is accurately known; and 3) Extensive characterization of the metabolic phenotype of the animals is feasible. PUBLIC RELEVANCE (provided by applicant): The specific objectives of this proposal are to characterize changes in gene and protein expression levels in the hypothalamus that are associated with obesity in the NHP. While these studies are primarily descriptive and correlative, this information will provide a critical database for the general scientific community and will allow both clinical and basic scientists to generate novel specific hypothesis about systems within the brain that become dysfunctional in association with obesity. These data will also provide key insights for the future development of therapeutics for the treatment of metabolic diseases

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 描述（由申请人提供）：肥胖症是全球健康的附季，是冠心病，高血压，脂肪肝病和2型糖尿病的增加的主要因素。在过去的30年中，美国成年人和儿童的超重和肥胖症患病率都在增加。现在，大约有6000万成年人（占成年人口的30％）现在肥胖。众所周知，许多因素有助于肥胖和相关疾病的发展。啮齿动物模型中的临床研究和研究都为肥胖和相关疾病的发病机理及其对大脑的影响提供了关键的见解。但是，人类和啮齿动物的研究受到限制，可能会阻碍其直接转化为有效的治疗干预措施。非人类灵长类动物（NHP）已成为整合啮齿动物和人类研究数据的关键模型。该建议的总体目的是在高脂饮食诱导的肥胖症（DIO）的NHP模型中进行平行的生理，基因组和蛋白质组学分析。该模型的主要优势是：1）NHP中DIO的进展与人类的发展相似，并且该模型发展了各种相关疾病； 2）仔细控制动物的实验环境，以使食物的摄入量准确； 3）动物的代谢表型的广泛表征是可行的。 公众相关性（由申请人提供）：该提案的具体目标是表征下丘脑中与NHP肥胖相关的基因和蛋白质表达水平的变化。尽管这些研究主要是描述性和相关性的，但该信息将为一般科学界提供关键的数据库，并允许临床和基础科学家对大脑内部与肥胖症相关的大脑中的系统产生新颖的特定假设。这些数据还将为未来的治疗疗法的发展提供关键见解