Detecting changes in cervical microstructure with quantitative ultrasound

用定量超声检测宫颈微观结构的变化

基本信息

批准号：
7712775
负责人：
TIMOTHY J HALL
金额：
$ 22.25万
依托单位：
UNIVERSITY OF WISCONSIN-MADISON
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-09-07 至 2011-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7712775
关键词：
Anisotropy Anterior Behavior Biochemical Biochemistry Biomechanics Cervical Cervix Uteri Clinic Collaborations Collagen Computer software Cytoskeleton DNA Sequence Rearrangement Development Distal Evaluation External Canal Extracellular Matrix Frequencies Functional disorder Generations Goals Gynecology Hysterectomy Image Imaging Techniques Intervention Investigation Laser Scanning Microscopy Lead Length Location Measurement Mechanics Methods Microscopic Modeling Molecular Molecular Biology Monitor Patients Positioning Attribute Pregnancy Premature Birth Preparation Procedures Process Progesterone Prostaglandins Reproducibility Research Design Risk Sampling Scanning Specimen Structure Surface Techniques Technology Testing Therapeutic Tissues Transducers Ultrasonic Transducer Ultrasonography Work digital fortification in vivo innovation instrument novel novel therapeutics optical imaging pregnant public health relevance quantitative ultrasound research study retinal rods second harmonic success technology development tool

项目摘要

DESCRIPTION (provided by applicant): The objective of this proposal is to develop a quantitative ultrasound technique to noninvasively assess collagen in the uterine cervix in an attempt to build a comprehensive understanding of cervical extra- cellular matrix microstructure. We anticipate that an enhanced understanding of cervical microstructural changes will lead to targeted investigation of specific remodeling processes in pregnancy that will pro- mote understanding of cervical insufficiency (that leads to preterm delivery). This in turn will allow exploration of options for novel therapeutic strategies. PUBLIC HEALTH RELEVANCE: This is a proposal to adapt new quantitative ultrasound technology to image and quantify in vivo cervical microstructure; specifically, we will target collagen because it is the primary component of the cervical extracellular matrix and, through its changes during the cervical remodeling process during gestation, is primarily responsible for cervical strength. The proposed effort involves (a) determining the sensitivity of ultrasound to the changes in collagen alignment in the cervix, (b) testing quantitative ultrasound in isotropic and anisotropic phantoms as well as ex vivo cervical tissue in order to establish imaging pa-rameters for in vivo cervical scanning, and (c) confirming through nonlinear optical imaging that we are detecting collagen in the cervix. The ability to accurately image rearrangements in collagen alignment in the cervical microstructure would inform our concept of cervical function and thus the dysfunction that leads to preterm delivery.

描述（由申请人提供）：本提案的目的是开发一种定量超声技术来无创评估子宫颈中的胶原蛋白，以试图建立对子宫颈细胞外基质微观结构的全面了解。我们预计，对宫颈微观结构变化的进一步了解将有助于对妊娠期特定重塑过程进行有针对性的研究，从而促进对宫颈机能不全（导致早产）的了解。这反过来又将允许探索新的治疗策略的选择。公共健康相关性：这是一项采用新的定量超声技术对体内宫颈微观结构进行成像和量化的提案；具体来说，我们将针对胶原蛋白，因为它是宫颈细胞外基质的主要成分，并且通过妊娠期间宫颈重塑过程中的变化，主要负责宫颈强度。拟议的工作包括（a）确定超声波对宫颈胶原排列变化的敏感性，（b）在各向同性和各向异性模型以及离体宫颈组织中测试定量超声波，以便建立子宫颈内的成像参数。体内宫颈扫描，以及（c）通过非线性光学成像确认我们正在检测宫颈中的胶原蛋白。准确成像宫颈微观结构中胶原蛋白排列重排的能力将为我们了解宫颈功能以及导致早产的功能障碍提供依据。