Mental Retardation and Development Disabilities Research

精神发育迟滞和发育障碍研究

• 批准号: 7931514
• 负责人: MARC YUDKOFF
• 金额: $ 10万
• 依托单位: CHILDREN'S HOSP OF PHILADELPHIA
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-30 至 2010-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7931514
• 关键词: Mental; Retardation; Development; Disabilities; Research

DESCRIPTION (provided by applicant): The proposed Mental Retardation and Developmental Disabilities Research Center (MRDDRC) was established in 1990 and renewed in 1995 and 1999. It represents the collaboration of the Children's Hospital of Philadelphia and the University of Pennsylvania (Penn). In this renewal application, funds are requested to support five core facilities and New Program Development projects. The cores are: (A) Administrative Core, which provides overall management of the MRDDRC. It administers all educational programs, lecture series, and the New Program Development projects. It also oversees the proposed Mid-Atlantic Research Consortium, a new venture that would encourage more joint research at the MRDDRCs at Penn, Johns Hopkins, and Children's National Medical Center. (B) Biostatistics and Bioinformatics, which provides consultation with regard to study design, statistical analysis, and database design. A new emphasis is bioinformatics, which is crucial to the interpretation of data culled from chip array analysis of DNA. (C) Molecular Genetics, which provides a centralized facility for DNA sequencing, fluorescent fragment analysis, mutation detection with cosmig contigs, screen of YAC libraries, quantitative PCR analysis, gene mapping, cell culture, etc. (D) Analytical Neurochemistry and Spectroscopy, which provides expert and support with regard to biochemical analyses. The core facilitates a wide spectrum of neurochemical analysis, from brain tissue to living cells in vitro to the intact organism. It combines mass spectrometric analysis of stable isotopes, HPLC of biogenic amines and amino acids, positron emission tomography (PET) services and fluorescent assays in live cells. A new component is the proposed Proteomics Subcore that facilitates characterization of proteins of neurochemical interest. (E) Cellular Neuroscience, which provides support and advice with regard to confocal microscopy, cytoarchitectural analysis, and cell culture. A new component is the availability of techniques for optically imaging circuit function in slice preparations using voltage sensitive dyes. Finally, the core proposes to provide users with expertise for the development and preservation of mouse models (transgenic and knock-outs). These units service more than 96 NIH -funded projects with an aggregate annual value of more than $12 million (greater than $1.5 million from NICHD). The Center includes more than 60 Penn faculty members representing 15 departments at the School of Medicine, School of Veterinary Medicine, The School of Nursing, the Wistar Institute, and the College of Arts and Sciences.

描述（由申请人提供）：拟议的心理迟缓和发育障碍研究中心（MRDDRC）成立了1990年，并于1995年和1999年续签。它代表了费城儿童医院和宾夕法尼亚大学（宾夕法尼亚州）的合作。 在此续签应用程序中，要求资金支持五个核心设施和新的计划开发项目。 核心是：（a）行政核心，它提供了MRDDRC的总体管理。 它管理所有教育计划，讲座系列和新计划开发项目。 它还负责监督拟议的中大西洋研究联盟，这是一项新事业，该公司将鼓励在Penn，Johns Hopkins和儿童国家医疗中心的MRDDRCS进行更多的联合研究。 （b）生物统计学和生物信息学，它提供了有关研究设计，统计分析和数据库设计的咨询。 新的重点是生物信息学，这对于从DNA的芯片阵列分析中剔除的数据的解释至关重要。 （c）分子遗传学提供了用于DNA测序，荧光片段分析，使用Cosmig重叠群的突变检测，YAC库筛选，定量PCR分析，基因映射，细胞培养等的集中式设施。 核心促进了广泛的神经化学分析，从脑组织到活细胞在体外再到完整的生物体。 它结合了稳定同位素，生物胺和氨基酸的HPLC的质谱分析，正电子发射断层扫描（PET）服务以及活细胞中的荧光测定。 一个新的成分是提出的蛋白质组学子核，可促进神经化学兴趣蛋白的表征。 （e）有关共聚焦显微镜，细胞结构分析和细胞培养的支持和建议的细胞神经科学。 一个新组件是使用电压敏感染料在切片制备中光学成像电路函数的技术的可用性。 最后，核心建议为用户提供用于开发和保存鼠标模型（转基因和敲除）的专业知识。 这些单位服务超过96个由NIH资助的项目，年利价值超过1200万美元（来自NICHD的150万美元）。 该中心包括60多名宾夕法尼亚州教职员工，代表15个部门，医学院，兽医学院，护理学院，维斯塔尔研究所和艺术与科学学院。