Gene Targeting Core

基因打靶核心

• 批准号: 8001277
• 负责人: William C Aird
• 金额: $ 39.64万
• 依托单位: BETH ISRAEL DEACONESS MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-08-01 至 2015-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8001277
• 关键词: Endothelium; Gene Targeting; Goals; Individual; Knock-out; Knockout Mice; Mus; Plasmids; Reagent; Research Personnel; Technical Expertise; Techniques; Transgenic Organisms; in vivo; promoter; vector

The primary goal of the Gene Targeting core will be to make available to the investigators of individual projects all available expertise, facilities, techniques and technical support required for generating /-/p/ttargeted mice, knockout/knockin mice and BAC transgenics. In addition, the core will provide reagents and advice on how to construct plasmids for use in Hprt targeting. In the case of knockout/knockin and BAC transgenic approaches, the core will also provide technical expertise in constructing targeting vectors.

基因靶向核心的主要目标是向单个项目的调查人员提供所有可用的专业知识，设施，技术和技术支持，以生成/ - /p/ttargeted小鼠，敲除/敲除小鼠和BAC转基因。此外，核心将提供有关如何构建用于HPRT靶向的质粒的试剂和建议。在敲除/敲除和BAC转基因方法的情况下，核心还将在构建靶向向量方面提供技术专长。