Chicken embryonic stem cells

鸡胚胎干细胞

• 批准号: 10719082
• 负责人: Qilong Ying
• 金额: $ 33.1万
• 依托单位: UNIVERSITY OF SOUTHERN CALIFORNIA
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-07-19 至 2027-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10719082
• 关键词: Anser species; Back; Biological Assay; Birds; Breeding; Cell Line; Cell Lineage; Cells; Chick Embryo; Chicken Cells; Chickens; Chimera organism; Chimerism; Communities; Derivation procedure; Developmental Biology; Ducks; ES Cell Line; Embryo; Excision; Fibroblasts; Geese; Gene Expression Profiling; Gene Targeting; Generations; Genes; Genetic; Genetic Engineering; Germ Cells; Germ Layers; In Vitro; LIF gene; Meleagris gallopavo; Methods; Modeling; Mus; Production; Protein Kinase C Inhibitor; Quail; Rattus; Reporter; Research; Signal Pathway; Specific qualifier value; Structure of primordial sex cell; Struthio camelus; Study models; Technology; Testing; Time; Transforming Growth Factor beta; Transgenic Organisms; WNT Signaling Pathway; chicken egg; early embryonic stage; embryonic stem cell; germline stem cells; human disease; induced pluripotent stem cell; induced pluripotent stem cell technology; inhibitor; knockout gene; offspring; pluripotency; small molecule; small molecule inhibitor; stem cell differentiation; tool; transcription factor; transmission process; zebra finch

Abstract Chickens and their embryos provide a valuable research model for the study of developmental biology and human diseases. However, their application has been greatly limited due to the lack of tools to genetically modify chickens and other avian species. Recently, we have developed methods for the derivation of germline competent embryonic stem cells (ESCs) from chickens. The availability of germline competent chicken ESCs opens up a new avenue for producing genetically engineered chickens. In this project, we propose to establish an ESC-based platform for the efficient generation of chicken germline chimeras (Aim 1). We will also optimize methods for the generation of primordial germ cells (PGCs) from chicken ESCs. These chicken ESC-derived PGCs will be used to produce genetically modified chickens (Aim 2). In Aim 3, by taking the advantage of the chicken ESC culture condition we have established, we will develop methods for the generation of induced pluripotent stem cells (iPSCs) from chicken embryonic fibroblasts. Successful completion of these three aims will provide the avian research community with a powerful genetic toolkit for the production of transgenic and gene-targeted avian species and for the conservation of endangered avian species.

抽象的 鸡及其胚胎为发育生物学和动物学研究提供了有价值的研究模型。 人类疾病。然而，由于缺乏基因检测工具，它们的应用受到很大限制。 改造鸡和其他鸟类。最近，我们开发了种系衍生方法 来自鸡的有能力的胚胎干细胞（ESC）。种系鸡胚胎干细胞的可用性 开辟了生产基因工程鸡的新途径。在这个项目中，我们建议建立 一个基于 ESC 的平台，用于高效生成鸡种系嵌合体（目标 1）。我们也会优化 从鸡 ESC 中产生原始生殖细胞 (PGC) 的方法。这些鸡 ESC 衍生的 PGC 将用于生产转基因鸡（目标 2）。在目标 3 中，利用 我们已经建立了鸡ESC培养条件，我们将开发诱导性胚胎干细胞的产生方法 来自鸡胚胎成纤维细胞的多能干细胞（iPSC）。圆满完成这三个目标 将为鸟类研究界提供强大的遗传工具包，用于生产转基因和 基因靶向鸟类物种和濒临灭绝的鸟类物种的保护。