Coherence Imaging for Assessing Colorectal Neoplasia

用于评估结直肠肿瘤的相干成像

• 批准号: 7634639
• 负责人: Adam Wax
• 金额: $ 32.58万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-05-24 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7634639
• 关键词: Animals; Azoxymethane; Biophotonics; Cancer Etiology; Cancerous; Cessation of life; Chemopreventive Agent; Colon; Colorectal; Colorectal Cancer; Colorectal Neoplasms; Device or Instrument Development; Disease; Epithelial; Epithelium; Fiber Optics; Goals; Health; Health Status; Histopathology; Image; Intestines; Lesion; Light; Measurement; Methods; Modeling; Optical Methods; Pathology; Patients; Rattus; Research; Resected; Resolution; Screening for cancer; Screening procedure; Survival Rate; System; Technology; Tissues; United States; Woman; animal tissue; base; cancer diagnosis; carcinogenesis; colorectal cancer screening; design; human tissue; imaging modality; improved; instrument; instrumentation; men; research study; surveillance study

Colorectal cancer (CRC) is the third most diagnosed cancer in the United States among both men and women, with approximately 150,000 new cases expected in 2008. It is also the second leading cause of cancer deaths in the U.S. with nearly 50,000 expected to die from CRC this year. As with most cancers, early detection is the key to improved survival rates. The objective of this research is to improve early detection of CRC by developing new optical methods for non‐invasively assessing the health status of colorectal tissues. To achieve this goal, we propose to develop complimentary coherence imaging methods for screening and surveillance of colorectal epithelium. The new optical methods are based on the principle of coherence imaging. This key attribute of this imaging approach is that it enables depth resolved measurements with ~10 μm resolution up to a depth of ~1 mm. For screening, we will use our new white light OCT scheme that allows spectroscopic measurements in the visible spectrum; for surveillance methods, we will us our angle-resolved low coherence interferometry (a/LCI) approach that uses Fourier plane imaging to execute depth resolved nuclear morphology measurements. The long range goal of this research is to create a suite of coherence imaging tools for noninvasive assessment of colorectal tissue health. To achieve this goal, we propose the following specific aims: 1) Instrument development. We will design and implement new coherence imaging instrumentation to enable examination of colorectal tissues for ex vivo tissue studies. 2) Animal studies. We will execute experiments with animal tissues to define the capabilities of both coherence imaging methods for assessing the pathology of colorectal epithelial tissues. We have selected the azoxymethane (AOM) induced rat carcinogenesis model, which is commonly used for chemopreventive studies of CRC. 3) Ex vivo human tissue studies. We will undertake studies of ex vivo human tissues to demonstrate that a) a/LCI technology can detect pre‐cancerous lesions in colorectal tissues from patients with irritable bowel disease (IBD) and b) our white light OCT method will generates suitable tissue images for co-registration purposes.

结直肠癌 (CRC) 是美国男性和女性中第三大诊断癌症，预计 2008 年将有约 150,000 个新病例。它也是美国癌症死亡的第二大原因，预计将有近 50,000 人死于结直肠癌。与今年的结直肠癌一样，早期发现是提高生存率的关键。 本研究的目的是通过开发新的光学方法来非侵入性评估结直肠组织的健康状况，以改善结直肠癌的早期检测。为了实现这一目标，我们建议开发用于筛查和监测结直肠上皮的互补相干成像方法。 新的光学方法基于相干成像原理，这种成像方法的关键属性是它能够以约 10 微米的分辨率进行深度分辨测量，深度可达约 1 毫米。对于筛选，我们将使用我们的新白光。光 OCT 方案允许在可见光谱中进行光谱测量；对于监视方法，我们将使用我们的 角度分辨低相干干涉测量 (a/LCI) 方法，使用傅立叶平面成像来执行深度分辨核形态测量。 这项研究的长期目标是创建一套用于无创评估结直肠组织健康的相干成像工具。为了实现这一目标，我们提出以下具体目标： 1）仪器开发。我们将设计和实施新的相干成像仪器，以便能够检查结直肠组织以进行离体组织研究。 2）动物研究。我们将用动物组织进行实验，以确定两种相干成像方法评估结直肠上皮组织病理学的能力。我们选择了氧化偶氮甲烷（AOM）诱导的大鼠致癌模型，该模型通常用于化学预防研究。 CRC 的。 3) 离体人体组织研究 我们将对离体人体组织进行研究，以证明 a) a/LCI 技术可以检测肠易激病 (IBD) 患者的结直肠组织中的癌前病变，以及 b) 我们的白光OCT 方法将生成适合共同配准目的的组织图像。