Structure and function of cytochrome b561

细胞色素b561的结构和功能

基本信息

批准号：
7781333
负责人：
Graham A. Palmer
金额：
$ 28.12万
依托单位：
RICE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
起止时间：
2007-04-02 至 2013-02-28
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7781333
关键词：
Address Adrenal Glands American Heart Association Amino Acids Antibodies Ascorbic Acid Bacteria Binding Biochemical Biological Biological Assay Catecholamines Charge Cysteine Cytochrome a Cytochromes Cytochromes b Development Dopamine Electron Transport Electrons Engineering Epinephrine Family member Funding Goals Heme Histidine Human Individual Intestines Investigation Iron Kinetics Laboratories Lanthanoid Series Elements Lead Ligands Link Measures Membrane Membrane Potentials Mixed Function Oxygenases Modeling Molecular Mutation Norepinephrine Oxidants Oxidation-Reduction Pathology Pathway interactions Phase Physiological Physiology Play Positioning Attribute Process Property Protein Family Proteins Pulse Radiolysis Reaction Reagent Recombinant Proteins Recombinants Reducing Agents Regulation Relaxation Research Personnel Rice Role Side Signal Transduction Structural Protein Structure Surface System Techniques Testing Texas Tumor Suppression analog ascorbate cytochrome b561 falls member mutant oxidation peptide hormone polypeptide programs prototype research study semidehydroascorbate

项目摘要

DESCRIPTION (provided by applicant): Adrenal ascorbate-dependent cytochrome (cyt.) b561 is a transmembrane electron transporter required for synthesis of catecholamine and some peptide hormone and is the prototype of an expanding and insufficiently characterized cyt. b561 protein family; other mammalian family members have been linked to intestinal iron transport and tumor suppression. Our overall goal is to characterize the mechanism of adrenal b561 and to use information about this cytochrome to understand the structure and function of other b561 family members. The specific aims of this project are: 1) Define the structural kernel of adrenal cyt. b561, determine its relationship to the membrane bilayer, and evaluate the kernel's generality among mammalian cyt. b561 family members; 2) Characterize the interaction of adrenal cyt. b561 with its natural reductant, ascorbate, and its natural oxidant, semidehydroascorbate; and 3) Determine the path of electron flow via adrenal cyt. b561 and its regulation. Planned experiments will be conducted on the native mammalian protein and recombinant wild type and mutant forms of the adrenal cytochrome and other mammalian cyt. b561 family members in prokaryotic and eukaryotic systems that we have developed. We will characterize the topological, structural, and kinetic properties of these recombinant proteins and purified endogenous adrenal cyt b561 using biochemical and biophysical techniques. Understanding how adrenal cyt. b561 functions is a fundamental part of understanding the physiology and pathology of epinephrine and norepinephrine and of some peptide hormones in humans. Defining similarities and differences between adrenal cyt. b561 and recently-discovered human cyt. b561 family members will accelerate characterization of the physiological and pathological roles of the newer cyt. b561s.

描述（由申请人提供）：肾上腺抗坏血酸依赖性细胞色素（Cyt。）B561是一种跨膜电子转运蛋白，用于合成儿茶酚胺和某些肽激素，并且是扩展和表征不足的cyt的原型。 B561蛋白家族；其他哺乳动物家庭成员与肠道铁运输和抑制肿瘤有关。我们的总体目标是表征肾上腺B561的机制，并使用有关该细胞色素的信息来了解其他B561家族成员的结构和功能。该项目的具体目的是：1）定义肾上腺细胞的结构核。 B561，确定其与膜双层的关系，并评估哺乳动物细胞中核的一般性。 B561家庭成员； 2）表征肾上腺细胞的相互作用。 B561及其天然还原剂，抗坏血酸及其天然氧化剂，半羟抗盐； 3）确定通过肾上腺细胞的电子流的路径。 B561及其法规。计划实验将对肾上腺细胞色素和其他哺乳动物细胞的天然哺乳动物蛋白和重组野生型和突变形式进行。 B561我们已经开发的原核生物和真核系统的家庭成员。我们将使用生物化学和生物物理技术来表征这些重组蛋白的拓扑，结构和动力学特性以及纯化的内源性肾上腺细胞Cyt B561。了解肾上腺细胞如何。 B561功能是理解肾上腺素和去甲肾上腺素的生理和病理学以及人类某些肽激素的基本组成部分。定义肾上腺细胞的相似性和差异。 B561和最近发现的人类Cyt。 B561家庭成员将加速对新Cyt的生理和病理作用的表征。 B561S。

项目成果

期刊论文数量（8）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Spectroscopic evidence of the role of an axial ligand histidinate in the mechanism of adrenal cytochrome b(561).

轴向配体组氨酸在肾上腺细胞色素 b(561) 机制中作用的光谱证据。

DOI：
10.1021/bi301127k
发表时间：
2012
期刊：
Biochemistry
影响因子：
2.9
作者：
daSilva,GiordanoFZ;Shinkarev,VladimirP;Kamensky,YuryA;Palmer,Graham
通讯作者：
Palmer,Graham

Electrochemical reduction of ferrous alpha-verdoheme in complex with heme oxygenase-1.

亚铁 α-绿血红素与血红素加氧酶-1 复合物的电化学还原。

DOI：
10.1016/j.jinorgbio.2007.05.016
发表时间：
2007
期刊：
Journal of inorganic biochemistry
影响因子：
3.9
作者：
Sato,Hideaki;Higashimoto,Yuichiro;Sakamoto,Hiroshi;Sugishima,Masakazu;Takahashi,Kenichi;Palmer,Graham;Noguchi,Masato
通讯作者：
Noguchi,Masato

High-yield production, purification and characterization of functional human duodenal cytochrome b in an Escherichia coli system.

在大肠杆菌系统中高产生产、纯化和表征功能性人十二指肠细胞色素 b。