NTHi Immunity in Young Children

幼儿的 NTHi 免疫力

基本信息

批准号：
7844257
负责人：
MICHAEL E PICHICHERO
金额：
$ 6.88万
依托单位：
ROCHESTER GENERAL HOSPITAL (NY)
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-06-01 至 2011-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7844257
关键词：
Acoustics Acute Age Antibodies Antibody Formation Antibody Repertoire Avidity Bacterial Adhesins Bacterial Infections Blood Child Childhood Clinical Clinical Trials Collaborations Developed Countries Developing Countries Diagnostic Disease Educational workshop Elements Event Future Goals Haemophilus influenzae ompP2 protein Health Heterogeneity Human Immune Immune response Immunity Intervention Knowledge Lead Liquid substance Modeling Moraxella (Branhamella) catarrhalis Morbidity - disease rate Nasal Lavage Fluid National Institute of Allergy and Infectious Disease Nontypable Haemophilus influenza Organism Otitis Otitis Media Otoscopy Pathogenesis Prevention Process Proteins Public Health Recording of previous events Recruitment Activity Research Research Personnel Risk Role Sampling Serum Site Streptococcus pneumoniae Technology Time Tympanometry Tympanostomy United States National Institutes of Health Vaccines cohort diagnostic accuracy experience insight middle ear prevent repository response suburb time interval vaccine candidate vaccine development

项目摘要

DESCRIPTION (provided by applicant): Nontypable Haemophilus influenzae (NTHi) is one of the major etiological agents of acute otitis media (AOM) which has very high morbidity among children in both developing and developed countries. The long term goal of this research is to better understand NTHi pathogenesis and human immune responses following nasopharyngeal (NP) colonization and AOM, in order to develop strategies for intervention in the disease. We will prospectively collect NP, middle ear fluids (MEF), and sera from young children age 2 to 24 months, who are otitis prone, have infrequent otitis, or remain otitis free. We propose to evaluate the role of specific immune responses to NTHi outer membrane proteins P2, P5, P6, and OMP26 in clearance of NP colonization by comparing children who clear colonization with those who experience prolonged colonization. In addition, we will establish a repository of prospectively collected mucosal and serum samples for other researchers in otitis media. The specific aims of this research are: Specific Aim 1: To prospectively collect NP secretions, MEF, and sera from young children who are otitis prone, have infrequent otitis, or remain otitis free. Specific Aim 2: To evaluate the role of specific immune responses to NTHi P2, P5, P6, and OMP26 in clearance of AOM by comparing children with different AOM experiences and in clearance of NP colonization by comparing children with different colonization experiences. Specific Aim 3: To establish a repository of prospectively collected mucosal and serum samples for future research in otitis media. Relevance to Public Health: This project is specifically responsive to research needs identified at an NIAID and NIDCD-organized workshop held at the NIH on 8-9 June 2004 on "Vaccines for Otitis Media: Proposals for Overcoming Obstacles to Progress." The results gained from these studies will provide insight to the immune mechanisms involved in the pathogenesis of otitis media aid in the development of a vaccine for otits media.

描述（由申请人提供）：不可分型流感嗜血杆菌（NTHi）是急性中耳炎（AOM）的主要病原体之一，在发展中国家和发达国家的儿童中发病率非常高。这项研究的长期目标是更好地了解 NTHi 发病机制以及鼻咽 (NP) 定植和 AOM 后的人体免疫反应，以便制定干预该疾病的策略。我们将前瞻性地收集 2 至 24 个月大的幼儿的 NP、中耳液 (MEF) 和血清，这些幼儿易患中耳炎、很少患中耳炎或没有中耳炎。我们建议通过比较清除定植的儿童和经历长期定植的儿童，评估对 NTHi 外膜蛋白 P2、P5、P6 和 OMP26 的特异性免疫反应在清除 NP 定植中的作用。此外，我们将为其他中耳炎研究人员建立前瞻性收集的粘膜和血清样本的存储库。本研究的具体目标是：具体目标 1：前瞻性地收集易患中耳炎、不常患中耳炎或保持无中耳炎的幼儿的 NP 分泌物、MEF 和血清。具体目标 2：通过比较不同 AOM 经历的儿童，评估针对 NTHi P2、P5、P6 和 OMP26 的特异性免疫反应在 AOM 清除中的作用，并通过比较不同定植经历的儿童，评估对 NP 定植清除的作用。具体目标 3：建立前瞻性收集的粘膜和血清样本库，用于未来中耳炎的研究。与公共卫生的相关性：该项目专门满足 2004 年 6 月 8 日至 9 日在 NIH 举办的 NIAID 和 NIDCD 组织的研讨会上确定的研究需求，主题为“中耳炎疫苗：克服进展障碍的建议”。这些研究获得的结果将深入了解中耳炎发病机制中涉及的免疫机制，有助于中耳炎疫苗的开发。