NTHi Immunity in Young Children

幼儿的 NTHi 免疫力

• 批准号: 7364550
• 负责人: MICHAEL E PICHICHERO
• 金额: $ 46.82万
• 依托单位: UNIVERSITY OF ROCHESTER
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-03-01 至 2009-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7364550
• 关键词: Acoustics; Acute; Age; Antibodies; Antibody Formation; Antibody Repertoire; Avidity; Bacterial Adhesins; Bacterial Infections; Blood; Child; Childhood; Clinical; Clinical Trials; Collaborations; Developed Countries; Developing Countries; Diagnostic; Disease; Educational workshop; Elements; Event; Future; Goals; Health; Heterogeneity; Human; Immune; Immune response; Immunity; Intervention; Knowledge; Lead; Liquid substance; Membrane Proteins; Modeling; Moraxella (Branhamella) catarrhalis; Morbidity - disease rate; Nasal Lavage Fluid; National Institute of Allergy and Infectious Disease; Nontypable Haemophilus influenza; Organism; Otitis; Otitis Media; Otoscopy; Pathogenesis; Personal Satisfaction; Prevention; Process; Proteins; Public Health; Recording of previous events; Recruitment Activity; Research; Research Personnel; Risk; Role; Sampling; Serum; Site; Streptococcus pneumoniae; Technology; Time; Tympanometry; Tympanostomy; United States National Institutes of Health; Vaccines; cohort; diagnostic accuracy; experience; insight; middle ear; prevent; repository; response; time interval; vaccine development

DESCRIPTION (provided by applicant): Nontypable Haemophilus influenzae (NTHi) is one of the major etiological agents of acute otitis media (AOM) which has very high morbidity among children in both developing and developed countries. The long term goal of this research is to better understand NTHi pathogenesis and human immune responses following nasopharyngeal (NP) colonization and AOM, in order to develop strategies for intervention in the disease. We will prospectively collect NP, middle ear fluids (MEF), and sera from young children age 2 to 24 months, who are otitis prone, have infrequent otitis, or remain otitis free. We propose to evaluate the role of specific immune responses to NTHi outer membrane proteins P2, P5, P6, and OMP26 in clearance of NP colonization by comparing children who clear colonization with those who experience prolonged colonization. In addition, we will establish a repository of prospectively collected mucosal and serum samples for other researchers in otitis media. The specific aims of this research are: Specific Aim 1: To prospectively collect NP secretions, MEF, and sera from young children who are otitis prone, have infrequent otitis, or remain otitis free. Specific Aim 2: To evaluate the role of specific immune responses to NTHi P2, P5, P6, and OMP26 in clearance of AOM by comparing children with different AOM experiences and in clearance of NP colonization by comparing children with different colonization experiences. Specific Aim 3: To establish a repository of prospectively collected mucosal and serum samples for future research in otitis media. Relevance to Public Health: This project is specifically responsive to research needs identified at an NIAID and NIDCD-organized workshop held at the NIH on 8-9 June 2004 on "Vaccines for Otitis Media: Proposals for Overcoming Obstacles to Progress." The results gained from these studies will provide insight to the immune mechanisms involved in the pathogenesis of otitis media aid in the development of a vaccine for otits media.

描述（由申请人提供）：不富裕的流感嗜血杆菌（NTHI）是急性耳炎培养基（AOM）的主要病因学药之一，在发展中国家和发达国家的儿童中的发病率很高。这项研究的长期目标是更好地了解鼻咽（NP）定植和AOM后NTHI发病机理和人类免疫反应，以制定干预疾病的策略。我们将前瞻性地收集2至24个月的年轻儿童的NP，中耳液（MEF）和血清，这些儿童容易发生中耳炎，不经常出现中耳炎或保持不含冲刺。我们建议评估对NTHI外膜蛋白P2，P5，P6和OMP26的特定免疫反应在清除NP定殖的情况下的作用，通过将清除定殖的儿童与经历延长定殖的孩子进行比较。此外，我们还将建立一个前瞻性收集的粘膜和血清样品的存储库，用于其他研究人员中的其他研究人员。这项研究的具体目的是：具体目的1：从易患中耳炎的年幼儿童中，前瞻性地收集NP分泌物，MEF和血清，患有不经常性的中耳炎或保持不含中耳炎。具体目的2：通过比较具有不同AOM经验的儿童，通过比较具有不同殖民经验的儿童，评估对NTHI P2，P5，P6和OMP26的特定免疫反应在清除AOM中的作用。特定目的3：建立一个前瞻性收集的粘膜和血清样品的存储库，以在中耳炎中进行未来的研究。与公共卫生的相关性：该项目特别响应于2004年6月8日至9日在NIH举行的NIAID和NIDCD组织的研讨会上确定的研究需求，涉及“耳炎媒体的疫苗：克服进步障碍的建议”。从这些研究中得出的结果将为OTIT培养基的疫苗开发中耳炎的发病介绍带来的免疫机制提供洞察力。