Laser Scanning Cytometer

激光扫描细胞仪

• 批准号: 7595975
• 负责人: Steven A Porcelli
• 金额: $ 39.84万
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-12-22 至 2009-12-21
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7595975
• 关键词: Area; Autoimmune Diseases; Cancer Biology; Cancer Center; Cells; Communicable Diseases; Cultured Cells; Data; Development; Ensure; Faculty; Flow Cytometry; Fluorescence; Funding; Housing; Hybrids; Image; Image Analysis; Immunology; Institution; Label; Laboratories; Lasers; Life; Microscope; Microscopic; Microscopy; Monitor; Operative Surgical Procedures; Request for Applications; Research Personnel; Research Project Grants; Sampling; Scanning; Science; Signal Transduction; Slide; Specimen; Staging; Supervision; Surface; Technology; Time; Tissues; Translational Research; Tube; cell fixing; detector; experience; instrument; lens; meetings; member; new technology; photomultiplier; public health relevance; Area; Autoimmune Diseases; Cancer Biology; Cancer Center; Cells; Communicable Diseases; Cultured Cells; Data; Development; Ensure; Faculty; Flow Cytometry; Fluorescence; Funding; Housing; Hybrids; Image; Image Analysis; Immunology; Institution; Label; Laboratories; Lasers; Life; Microscope; Microscopic; Microscopy; Monitor; Operative Surgical Procedures; Request for Applications; Research Personnel; Research Project Grants; Sampling; Scanning; Science; Signal Transduction; Slide; Specimen; Staging; Supervision; Surface; Technology; Time; Tissues; Translational Research; Tube; cell fixing; detector; experience; instrument; lens; meetings; member; new technology; photomultiplier; public health relevance

DESCRIPTION (provided by applicant): We are requesting funds for a Compucyte iCys Laser Scanning Cytometer (LSC). The LSC is a relatively recently developed instrument that joins many of the advantages of traditional microscopy and flow cytometry, and significantly extends the limits of single cell analyses. Rather than randomly imaging an entire field like a microscope does, it selects, images and analyzes cells that meet certain adjustable criteria (such as size or fluorescence). Unlike conventional flow cytometry, the LSC is usually used to analyze cells fixed to horizontal surface, such as cultured cells on slides or in a variety of culture vessels. Live cells can be imaged and cytometrically analyzed for fluorescent signals, and a variety of types of fixed specimens can also be studied in this way. The instrument uses an automated nanostep X-Y stage to move samples through the objective lens, applying up to three different lasers to excite fluorescent emissions and quantitating these using an array of four photomultiplier tube (PMT) detectors. Thus, the LSC is essentially a powerful hybrid technology that combines imaging and cytometric analysis in a single platform. Importantly, with the LSC image becomes a parameter, and detailed microscopic pictures are stored and referenced directly to cytometric data. A highly appealing feature of the instrument that we plan to use extensively is its ability to gather quantitative fluorescence data from microscopic areas of fluorescently labeled tissue sections, and to display and analyze such data in a manner that is comparable in many cases to true cytometric data such as one obtains with traditional flow cytometry. We plan to house and operate the iCys LSC in our well established central Flow Cytometry Core Laboratory, where there will be ample expertise and oversight to ensure that the instrument is used to its full capacity. The Core Laboratory has an experienced senior faculty member (Dr. Steven Porcelli) as its supervisor, and employs three full time faculty level associates to direct operations and provide technical supervision. There is also a range of internal consultants with expertise in areas of major relevance to the development of applications that will use the LSC. The Core Laboratory has a well developed and carefully monitored financial plan, and receives substantial institutional support from the institution and from our NCI-funded Cancer Center to ensure its efficient and stable operation. By incorporating the iCys LSC into this core laboratory, a large body of investigators pursuing many diverse NIH-funded basic and translational research projects will gain convenient and affordable access to this important new technology. Public Health Relevance: This application requests funding that will allow our Flow Cytometry Core Laboratory to acquire a new analytical instrument called the Compucyte iCys Laser Scanning Cytometer. This instrument will provide many researchers at our institution with a powerful new way to analyze cells and tissues. The studies that will be done with this instrument will advance our understanding of a wide spectrum of areas with high relevance to biomedical science, including cancer biology, immunology, autoimmune diseases and infectious diseases.

描述（由申请人提供）：我们要求用于Compucyte ICYS激光扫描细胞仪（LSC）的资金。 LSC是一种相对开发的仪器，它连接了传统显微镜和流式细胞术的许多优势，并显着扩展了单细胞分析的限制。它没有像显微镜那样随机对整个领域进行成像，而是选择，图像和分析符合某些可调标准（例如尺寸或荧光）的细胞。与常规流式细胞术不同，LSC通常用于分析固定在水平表面的细胞，例如幻灯片上的培养细胞或各种培养容器。可以对荧光信号进行成像和细胞量学分析，并且也可以以这种方式研究各种类型的固定样品。该仪器使用自动的纳米棒X-y级通过物镜将样品移动，最多使用三个不同的激光来激发荧光排放，并使用四个光电倍增管（PMT）检测器的阵列对其进行定量。因此，LSC本质上是一种功能强大的混合技术，可在单个平台中结合成像和细胞术分析。重要的是，使用LSC图像成为参数，并存储详细的显微镜图片并直接引用了细胞术数据。我们计划广泛使用该仪器的一个高度吸引人的特征是它的能力是从荧光标记的组织切片的显微镜区域收集定量荧光数据，并以许多情况在许多情况下与真实的细胞仪数据相提并论的方式，例如通过传统的流式序列化获得和分析此类数据。我们计划在我们建立的中央流式细胞仪核心实验室中容纳和操作ICYS LSC，在那里将有足够的专业知识和监督，以确保该仪器充分使用。核心实验室拥有一位经验丰富的高级教师（Steven Porcelli博士）为其主管，并雇用三名全职教职员工来指导运营并提供技术监督。还有一系列内部顾问在与使用LSC的应用程序的开发方面具有专业知识。核心实验室拥有一项良好的发达和仔细监控的财务计划，并从机构和我们的NCI资助的癌症中心获得了大量的机构支持，以确保其有效稳定的运营。通过将ICYS LSC纳入该核心实验室，众多追求NIH资助的基本和转化研究项目的调查人员将获得方便且负担得起的新技术。公共卫生相关性：此应用程序要求提供资金，以使我们的流式细胞仪核心实验室获得一种新的分析工具，称为Compucyte ICYS激光扫描细胞仪。该工具将为我们机构的许多研究人员提供一种有力的新方法来分析细胞和组织。将使用该工具进行的研究将提高我们对与生物医学科学相关的广泛领域的理解，包括癌症生物学，免疫学，自身免疫性疾病和传染病。