In Vivo Optical Imaging Scanner for Small Animal Research

用于小动物研究的体内光学成像扫描仪

• 批准号: 7596702
• 负责人: STUART S. BERR
• 金额: $ 36.01万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-01-01 至 2009-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7596702
• 关键词: Animal Experimentation; Animals; Arts; Biological; Biological Process; Biological Sciences; Bioluminescence; Brain; Computational Technique; Development; Disease; Engineering; Event; Fingers; Fluorescence; Functional Imaging; Funding; Half-Life; Heart Diseases; Housing; Human; Human body; Image; Imagery; Imaging Device; Imaging Techniques; Lung Inflammation; Magnetic Resonance; Malignant Neoplasms; Measurement; Methodology; Molecular; Optics; Organism; Positron-Emission Tomography; Public Health; Radionuclide Imaging; Research; Resolution; Resources; Scanning; Sentinel Lymph Node; Surface; System; Techniques; Three-Dimensional Image; Universities; Upper arm; Virginia; X-Ray Computed Tomography; base; diffuse optical tomography; fluorescence imaging; human subject; imaging modality; imaging probe; in vivo; malignant breast neoplasm; medical schools; molecular imaging; next generation; optical imaging; programs; public health relevance; reconstruction; single photon emission computed tomography; tomography

DESCRIPTION (provided by applicant): The University of Virginia (UVa) Schools of Medicine and Engineering have established successful research programs in the development and application of imaging methodologies and techniques for non- invasive serial studies of the molecular mechanisms underlying infectious diseases1, 2 heart disease 3-5, lung inflammation 6 and cancer 7, 8 amongst many others in small animals and humans. Imaging is carried out at the UVa Molecular Imaging Center (UVaMIC) that houses state of the art imaging devices including magnetic resonance (MR), X-ray computed tomography (CT), planar fluorescence and bioluminescence imaging (BLI), single gamma emission tomography (SPECT), and positron emission tomography (PET) scanners. Bioluminescence and fluorescence imaging 9-11 have emerged as high sensitivity and high throughput imaging modalities for targeted molecular imaging. We are requesting funds for a next generation high sensitivity and high-resolution small animal optical imager with 3D reconstruction capabilities based on a computational technique called diffuse optical tomography. We propose to purchase the SPECTRUM, small animal fluorescence and bioluminescence optical scanner from Caliper Life Sciences, Inc. The present IVIS 100 scanner from Xenogen (now a part of Caliper Life Sciences, Inc.) has a number of limitations, which would be overcome by the proposed new optical scanner in the UVaMIC. The main benefits of the new system will be better fluorescence imaging and the ability to create 3D images. Full utilization of the enhanced capabilities afforded by the SPECTRUM small animal optical scanner will be assured through an existing comprehensive technical, scientific, and administrative plan. PUBLIC HEALTH RELEVANCE: We are requesting funds for a state of art bioluminescence and fluorescence small animal scanner, which is a necessary resource for the University of Virginia (UVa) program in molecular imaging. Molecular imaging is the "visualization, characterization, and measurement of biological processes at the molecular and cellular levels in humans and other living systems."12 This probing by imaging of biological processes can be used to localize and quantify particular events. Optical imaging is a preferred functional imaging modality over Positron Emission Tomography (PET) or Single Gamma Emission Tomography (SPECT) due to its high sensitivity and throughput. Moreover, molecular events in an animal subject could be non-invasively studied over days due to longer biological half-life as compared to radionuclide imaging where half-life is a major constraint. However, a combination of optical and PET and/or SPECT could enable both high sensitivity and high resolution imaging of molecular events in animal subjects and humans. Although whole body human scanning is unlikely, the feasibility of the diffuse optical tomography technique for imaging breast cancer, superficial sentinel lymph nodes, the brain surface, arms and fingers has been shown. The relevance to public health of the addition of the next generation bioluminescence and fluorescence scanner to the already extensive imaging resources available at UVaMIC is that the presence of this scanner will enable research that is now not possible on new ways of detecting, characterizing, and treating diseases utilizing optical scanning.

描述（由申请人提供）：弗吉尼亚大学（UVA）医学和工程学学校已建立了成功的研究计划，以开发和应用成像方法和技术，用于对传染病的分子机制的非侵入性连续研究1，2心脏病3-5 3-5，肺部炎症6和癌症7，8，8，其他小动物和小动物中的8个。成像是在UVA分子成像中心（UVAMIC）进行的，该中心包含最先进的成像设备，包括磁共振（MR），X射线计算机断层扫描（CT），Planar荧光和生物发光成像（BLI），单Gamma发射器（BLI），单个Gamma发射器（Spect）和Potitron Potitron发射器（PET）发射器（PET）。生物发光和荧光成像9-11已成为针对靶向分子成像的高灵敏度和高吞吐量成像。我们要求基于3D重建功能的下一代高灵敏度和高分辨率小动物光学成像仪的资金，该计算能力基于一种称为“弥漫性光学断层扫描”的计算技术。我们建议从Caliper Life Sciences，Inc。购买频谱，小动物荧光和生物发光光学扫描仪。现有的IVIS 100 Xenogen（现在是Caliper Life Sciences，Inc。的一部分）有许多限制，这将由uVamic的拟议的新光学扫描仪克服。新系统的主要好处将是更好的荧光成像和创建3D图像的能力。通过现有的全面技术，科学和行政计划，将确保光谱小动物光学扫描仪提供的增强功能的全面利用。 公共卫生相关性：我们要求为一种最先进的生物发光和荧光小动物扫描仪提供资金，这是弗吉尼亚大学（UVA）分子成像计划的必要资源。分子成像是“人类和其他生物系统中分子和细胞水平上生物过程的可视化，表征和测量。” 12通过对生物过程的成像进行探测，可以用于定位和量化特定事件。光学成像是由于其高灵敏度和吞吐量而与正电子发射断层扫描（PET）或单个伽马发射断层扫描（SPECT）相比的优选功能成像方式。此外，与放射性核素成像相比，由于生物半衰期较长，因此在几天内，可以对动物受试者中的分子事件进行非侵入性研究，而半衰期是主要约束。但是，光学和/或SPECT的结合可以使动物受试者和人类中分子事件的高灵敏度和高分辨率成像。尽管不太可能进行全身扫描，但已经显示了用于成像乳腺癌，浅表哨兵淋巴结，脑表面，手臂和手指的弥漫性光学断层扫描技术的可行性。与公共卫生相关的是，在UVAMIC可获得的已有广泛的成像资源中，将下一代生物发光和荧光扫描仪的添加相关的是，该扫描仪的存在将使现在无法在检测，表征和治疗利用光学扫描的疾病的新方法上进行研究。

项目成果

Inhibition of neurotensin receptor 1 selectively sensitizes prostate cancer to ionizing radiation.

• DOI: 10.1158/0008-5472.can-11-1646
• 发表时间: 2011-09
• 期刊: Cancer research
• 影响因子: 11.2
• 作者: Nicholas C. K. Valerie;E. Casarez;J. DaSilva;M. Dunlap-Brown;S. Parsons;G. Amorino;J. Dziegielewski