Adaptive Optics Imager for Study of Pediatric Retinal Disorders

用于研究儿童视网膜疾病的自适应光学成像仪

• 批准号: 7595302
• 负责人: ANNE B FULTON
• 金额: $ 36.5万
• 依托单位: BOSTON CHILDREN'S HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-04-01 至 2010-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7595302
• 关键词: Adolescent; Affect; Albinism; Area; Biology; Blindness; Blood Vessels; Caring; Childhood; Collaborations; Data; Development; Disease; Elements; Eye; Funding; Genetic; Genotype; Image; Individual; Iron; Knowledge; Lead; Learning; Macular degeneration; Mediating; Minor; Modeling; Myopia; Neurons; Nuclear; Otolaryngology; Pathologic Nystagmus; Pathology; Patients; Photoreceptors; Premature Birth; Primates; Protein Structure Initiative; Rattus; Recording of previous events; Refractive Errors; Research; Research Personnel; Retina; Retinal; Retinal Diseases; Retinopathy of Prematurity; Side; Structure; Testing; United States National Institutes of Health; Usher Syndrome; Visual; adaptive optics; base; design; fovea centralis; improved; in vivo; insight; instrument; physical science; public health relevance; relating to nervous system; sample fixation; ultra high resolution

DESCRIPTION (provided by applicant): The broad, long term objective of this project is to extend our understanding of neuro-vascular relationships in the pediatric fovea, the area in the central retina that mediates the exquisite acuity in the healthy, mature eye. Neural and vascular elements participate in the development of the normal fovea both pre- and post-natally, and pediatric retinal disorders can alter the neuro-vascular relations during development. To test hypotheses about the fovea, our group of eight investigators requests an ultra-high resolution multimodal adaptive optics retinal imager ("PSI Retinal Imager"). This instrument enables non-invasive, in vivo acquisition of images of retinal cross-sections with nearly the same fidelity as traditional histological sections. It provides images showing nuclear, plexiform, and photoreceptor layers as well as en-face images of vasculature and neurons side-by-side at selected retinal depths. With current NIH funding, the major users investigate the eyes in pediatric subjects with a history of preterm birth (Fulton, VanderVeen, Leviton) and refractive errors in a primate model (Troilo). It is well established that retinopathy of prematurity (ROP), and even premature birth alone, alters the neural and vascular elements of the retina, but there is much more to learn about the photoreceptors and the perifoveal vasculature in subjects born preterm. The minor users' research broadens our understanding of the retina. Although the rat does not have a fovea, rat models of ROP provide an opportunity to examine the fundamental biology of neuro-vascular pathologies (Hansen). Collaborations with Genetics (Irons) and Otorhinolaryngology (Kenna) are designed to investigate the foveas in genotyped subjects with heritable conditions including albinism, Stargardt juvenile macular degeneration, and Usher syndrome which, like preterm birth and ROP, cause visual deficits in childhood. Incorporating the eye tracker into the PSI Retinal Imager is necessary for acquiring uncorrupted images from the latter subjects, as well as those with ROP who have nystagmus and/or unsteady fixation. Myopia and other significant refractive errors are common in subjects with these disorders. Thus, studies of the fovea in healthy individuals with myopia provide critical reference data against which data from subjects with retinal pathologies may be compared (Coletta). The PSI Retinal Imager will allow us to obtain quantitative information that will provide new insights into the neuro-vascular components of foveal disorders. We believe that this new knowledge will lead to better management of pediatric retina patients. PUBLIC HEALTH RELEVANCE: Investigators of retinal disorders request an ultra-high resolution Retinal Imager (Physical Sciences Inc.) for further studies of the fine structure of the pediatric fovea and neurovascular relations in the central retina. Their objectives are to gain new knowledge and insights into the basis for childhood blindness and visual loss caused by disorders affecting the central retina. New understanding will contribute to plans for improved care and treatment.

描述（由申请人提供）：该项目的广泛、长期目标是扩展我们对儿科中央凹神经血管关系的理解，中央凹是视网膜中央的区域，调节健康、成熟眼睛的敏锐度。 神经和血管元素参与出生前和出生后正常中央凹的发育，小儿视网膜疾病可以改变发育过程中的神经血管关系。 为了测试有关中央凹的假设，我们的八名研究人员小组需要一台超高分辨率多模态自适应光学视网膜成像仪（“PSI 视网膜成像仪”）。 该仪器能够以与传统组织学切片几乎相同的保真度，以非侵入性的体内方式采集视网膜横截面图像。 它提供显示核层、丛状层和光感受器层的图像，以及在选定的视网膜深度并排的脉管系统和神经元的正面图像。 在目前 NIH 的资助下，主要用户研究了有早产史的儿科受试者（Fulton、VanderVeen、Leviton）的眼睛和灵长类动物模型（Troilo）的屈光不正。 众所周知，早产儿视网膜病变（ROP），甚至早产本身都会改变视网膜的神经和血管成分，但对于早产受试者的光感受器和黄斑中心凹周围血管系统，还有更多的东西需要了解。 未成年用户的研究拓宽了我们对视网膜的了解。 尽管大鼠没有中央凹，但 ROP 大鼠模型提供了研究神经血管病理基础生物学的机会 (Hansen)。 与遗传学 (Irons) 和耳鼻喉科 (Kenna) 的合作旨在研究患有遗传性疾病的基因型受试者的中央凹，这些遗传性疾病包括白化病、Stargardt 青少年黄斑变性和 Usher 综合征，这些疾病与早产和 ROP 一样，会导致儿童期视力缺陷。 将眼动仪集成到 PSI 视网膜成像仪中对于从后者以及患有眼球震颤和/或不稳定注视的 ROP 患者获取未损坏的图像是必要的。 近视和其他严重的屈光不正在患有这些疾病的受试者中很常见。 因此，对患有近视的健康个体的中央凹的研究提供了关键的参考数据，可以将来自患有视网膜病变的受试者的数据进行比较（Coletta）。 PSI 视网膜成像仪将使我们能够获得定量信息，从而为中心凹疾病的神经血管成分提供新的见解。 我们相信，这一新知识将有助于更好地管理儿科视网膜患者。 公共健康相关性：视网膜疾病研究人员需要超高分辨率视网膜成像仪（Physical Sciences Inc.），以进一步研究儿童中央凹的精细结构和中央视网膜的神经血管关系。 他们的目标是获得新的知识和见解，了解影响中央视网膜的疾病引起的儿童失明和视力丧失的基础。 新的认识将有助于制定改善护理和治疗的计划。