Adaptive Optics Imager for Study of Pediatric Retinal Disorders

用于研究儿童视网膜疾病的自适应光学成像仪

• 批准号: 7595302
• 负责人: ANNE B FULTON
• 金额: $ 36.5万
• 依托单位: BOSTON CHILDREN'S HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-04-01 至 2010-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7595302
• 关键词: Adolescent; Affect; Albinism; Area; Biology; Blindness; Blood Vessels; Caring; Childhood; Collaborations; Data; Development; Disease; Elements; Eye; Funding; Genetic; Genotype; Image; Individual; Iron; Knowledge; Lead; Learning; Macular degeneration; Mediating; Minor; Modeling; Myopia; Neurons; Nuclear; Otolaryngology; Pathologic Nystagmus; Pathology; Patients; Photoreceptors; Premature Birth; Primates; Protein Structure Initiative; Rattus; Recording of previous events; Refractive Errors; Research; Research Personnel; Retina; Retinal; Retinal Diseases; Retinopathy of Prematurity; Side; Structure; Testing; United States National Institutes of Health; Usher Syndrome; Visual; adaptive optics; base; design; fovea centralis; improved; in vivo; insight; instrument; physical science; public health relevance; relating to nervous system; sample fixation; ultra high resolution

DESCRIPTION (provided by applicant): The broad, long term objective of this project is to extend our understanding of neuro-vascular relationships in the pediatric fovea, the area in the central retina that mediates the exquisite acuity in the healthy, mature eye. Neural and vascular elements participate in the development of the normal fovea both pre- and post-natally, and pediatric retinal disorders can alter the neuro-vascular relations during development. To test hypotheses about the fovea, our group of eight investigators requests an ultra-high resolution multimodal adaptive optics retinal imager ("PSI Retinal Imager"). This instrument enables non-invasive, in vivo acquisition of images of retinal cross-sections with nearly the same fidelity as traditional histological sections. It provides images showing nuclear, plexiform, and photoreceptor layers as well as en-face images of vasculature and neurons side-by-side at selected retinal depths. With current NIH funding, the major users investigate the eyes in pediatric subjects with a history of preterm birth (Fulton, VanderVeen, Leviton) and refractive errors in a primate model (Troilo). It is well established that retinopathy of prematurity (ROP), and even premature birth alone, alters the neural and vascular elements of the retina, but there is much more to learn about the photoreceptors and the perifoveal vasculature in subjects born preterm. The minor users' research broadens our understanding of the retina. Although the rat does not have a fovea, rat models of ROP provide an opportunity to examine the fundamental biology of neuro-vascular pathologies (Hansen). Collaborations with Genetics (Irons) and Otorhinolaryngology (Kenna) are designed to investigate the foveas in genotyped subjects with heritable conditions including albinism, Stargardt juvenile macular degeneration, and Usher syndrome which, like preterm birth and ROP, cause visual deficits in childhood. Incorporating the eye tracker into the PSI Retinal Imager is necessary for acquiring uncorrupted images from the latter subjects, as well as those with ROP who have nystagmus and/or unsteady fixation. Myopia and other significant refractive errors are common in subjects with these disorders. Thus, studies of the fovea in healthy individuals with myopia provide critical reference data against which data from subjects with retinal pathologies may be compared (Coletta). The PSI Retinal Imager will allow us to obtain quantitative information that will provide new insights into the neuro-vascular components of foveal disorders. We believe that this new knowledge will lead to better management of pediatric retina patients. PUBLIC HEALTH RELEVANCE: Investigators of retinal disorders request an ultra-high resolution Retinal Imager (Physical Sciences Inc.) for further studies of the fine structure of the pediatric fovea and neurovascular relations in the central retina. Their objectives are to gain new knowledge and insights into the basis for childhood blindness and visual loss caused by disorders affecting the central retina. New understanding will contribute to plans for improved care and treatment.

描述（由申请人提供）：该项目的广泛，长期目标是扩展我们对小儿中央凹的神经血管关系的理解，这是中央视网膜中心区域，它介导了健康，成熟眼睛中精致的敏锐度。 神经和血管元素在本质前后都参与正常中央凹的发展，而小儿视网膜疾病可以改变发育过程中神经血管关系。 为了测试有关中央凹的假设，我们的八组研究人员要求超高分辨率多模式自适应光学视网膜成像仪（“ PSI视网膜成像仪”）。 该仪器可实现无创的体内获取视网膜横截面图像，其保真度几乎与传统的组织学部分相同。 它提供了显示核，丛状和感光层的图像，以及在选定的视网膜深度处并排并排的脉管系统和神经元图像。 凭借当前的NIH资金，主要用户研究了具有早产史的小儿对象（Fulton，Vanderveen，Leviton）和灵长类动物模型中的折射错误。 众所周知，早产的视网膜病变（ROP），甚至单独出生，都会改变视网膜的神经和血管元素，但在出生早产的受试者中，还有更多关于感光体和perifoveal脉管系统的知识。 次要用户的研究扩大了我们对视网膜的理解。 尽管大鼠没有中央凹，但ROP的大鼠模型提供了检查神经血管病理学（Hansen）的基本生物学的机会。 与遗传学（IRON）和耳义核能学（KENNA）的合作旨在调查具有可遗传条件的基因分型受试者中的卵泡，包括白化病，Stargardt少年黄斑变性和Usher综合征，这些综合征和Usher综合征，这些综合症，例如早产和ROP，会导致童年的视觉缺陷。 将目光跟踪器纳入PSI视网膜成像仪中对于从后者受试者中获取未腐烂的图像以及具有Nystagmus和/或不稳定固定的ROP的图像是必要的。 在患有这些疾病的受试者中，近视和其他重大折射错误很常见。 因此，对近视健康个体的中央凹的研究提供了关键的参考数据，可以比较具有视网膜病理学受试者的数据（Coletta）。 PSI视网膜成像仪将使我们能够获得定量信息，这些信息将为中央凹疾病的神经血管成分提供新的见解。 我们认为，这种新知识将使小儿视网膜患者更好地管理。 公共卫生相关性：视网膜疾病的研究者要求进一步研究中央视网膜中小儿中央凹和神经血管关系的良好结构的超高分辨率视网膜成像仪（物理科学公司）。 他们的目标是获得新的知识和见解，以使影响中央视网膜的疾病引起的童年失明和视觉丧失。 新的理解将有助于改善护理和治疗的计划。