Becton Dickinson LSR II Flow Cytometer

Becton Dickinson LSR II 流式细胞仪

• 批准号: 7594876
• 负责人: BRUCE J SHENKER
• 金额: $ 27.38万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-04-01 至 2010-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7594876
• 关键词: 20 year old; Air; Area; Arts; Biology; Calcium; Cell Cycle; Cells; Communicable Diseases; Connective Tissue; Contract Services; Core Facility; Dental Schools; Disease; Equipment; Flow Cytometry; Fluorescence; Fluorochrome; Funding; Future; Immunity; Individual; Infection; Inflammation; Ions; Knowledge; Laboratories; Lasers; Lipids; Manufacturer Name; Medicine; Metabolism; Monitor; Mouth Diseases; Oral; Oral health; Pathogenesis; Pathologic; Periodontal Diseases; Physiological; Proteins; Research; Research Activity; Research Personnel; Research Support; Scientist; Signal Pathway; Stimulus; Surface; Technology; Tissues; United States National Institutes of Health; cell analyzer; craniofacial; flexibility; instrument; malignant mouth neoplasm; meetings; oral tissue; public health relevance; research facility; response; success

DESCRIPTION (provided by applicant): The short and long-term objective of this proposal is to upgrade the School of Dental Medicine (SDM) Flow Cytometry Core Facility so that it may continue to operate with state-of-the-art equipment. The facility is currently equipped with a 20 year-old instrument that can no longer be maintained on service contracts as it has been designated as obsolete by the manufacturer. Funds are requested to purchase a high-end flow cytometer; specifically, we plan to purchase a Becton Dickinson LSR II cell analyzer. The BD LSR II is an air-cooled, four laser (488nm, 633nm, 405nm and 355 nm) benchtop flow cytometer with the capability of acquiring 10 fluorescence parameters as configured. This configuration will provide the flexibility to meet the diverse needs of our user group. Moreover, it will support our investigators as they expand their studies to larger numbers of fluorochromes and thereby obtain significantly more information per cell. Investigators will be able to monitor: a wide range of both surface and intracellular markers, assess changes in cellular concentrations of ions such as calcium, cell cycle distribution, signaling pathways as well as study protein-protein and protein-lipid interactions. Core research facilities are vital to the success of research enterprises such as that at the SDM by providing investigators with access to advanced technologies in their research activities, which for financial reasons, would otherwise not be affordable to the individual scientist or laboratory. The SDM Flow Cytometry Facility supports the diverse needs of over 11 scientists whose research efforts are focused on advancing oral health. Supported projects are focused, in particular, on studies on the response of oral and craniofacial tissue to physiologic and pathologic stimuli with emphasis in three areas: inflammation and immunity, infectious disease, and the biology of soft and hard connective tissue. Public Health Relevance: The SDM Flow Cytometry Facility and the requested funds for acquisition of a state-of-the-art flow cytometer are vital to ongoing studies that elucidate pathogenic mechanisms in periodontal disease, herpetic infection, oral cancer and disorders associated with the metabolism of oral tissues, among others. Of particular relevance, this facility provides the necessary support for studies that are currently funded by the NIH as well as provide for an advantage for our investigators as they compete for future federal research support to advance our knowledge of the pathogenesis of oral disease.

描述（由申请人提供）：该提案的短期和长期目标是升级牙科医学学院（SDM）流式细胞仪核心设施，以便它可以继续使用最先进的设备运行。该设施目前配备了20年历史的仪器，由于制造商已将其指定为过时的服务合同。要求资金购买高端流式细胞仪；具体而言，我们计划购买Becton Dickinson LSR II细胞分析仪。 BD LSR II是一种气冷，四个激光器（488nm，633nm，405nm和355 nm）台式流式细胞仪，具有配置的10个荧光参数的能力。这种配置将提供灵活性，以满足我们用户组的各种需求。此外，它将在我们的研究人员将研究扩展到大量荧光染色体上，从而支持每个细胞的更多信息。研究者将能够监测：各种表面和细胞内标记，评估钙，细胞周期分布，信号通路以及研究蛋白质蛋白质和蛋白质脂质相互作用等离子的细胞浓度的变化。核心研究设施对于在SDM等研究企业的成功中至关重要，通过为研究人员提供在其研究活动中获得高级技术的访问，这是出于经济原因，否则该研究活动将使个人科学家或实验室负担不起。 SDM流式细胞仪设施支持11位以上的科学家的各种需求，他们的研究工作着重于提高口腔健康。受支持的项目尤其是针对口腔和颅面组织对生理和病理刺激的反应的研究，重点是三个领域：炎症和免疫，感染性疾病以及软和硬结缔组织的生物学。公共卫生相关性：SDM流式细胞仪设施和获得最先进的流式细胞仪所需的资金对于正在进行的研究至关重要，这些研究阐明了牙周疾病，疱疹感染，口腔癌，口腔癌和与口腔组织的代谢相关的致病机制。该设施特别相关，为目前由NIH资助的研究提供了必要的支持，并为我们的研究人员提供了优势，因为他们竞争未来的联邦研究支持，以促进我们对口腔疾病发病机理的了解。