COPAS SELECT Embryo Sorter

COPAS SELECT 胚胎分选机

• 批准号: 7586478
• 负责人: Deborah J Andrew
• 金额: $ 19.16万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-05-01 至 2010-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7586478
• 关键词: Animal Model; Area; Biochemistry; Caenorhabditis elegans; Cellular biology; Charge; Computational Molecular Biology; Development; Disease; Drosophila genus; Drosophila melanogaster; Education; Embryo; Event; Experimental Models; Fertilization; Funding; Genetic Screening; Genotype; Germ Cells; Grant; Housing; Human Biology; Individual; Injury; Kidney; Laboratories; Learning; Maintenance; Manuals; Mediating; Minor; Molecular; Morphogenesis; Myoblasts; Nematoda; Neurons; Organ; Organism; Physiology; Procedures; Replacement Therapy; Research; Research Training; Reverse Transcriptase Polymerase Chain Reaction; Sampling; Sensory; Signal Pathway; Signal Transduction; Site; Sorting - Cell Movement; Specialist; Specific qualifier value; Techniques; Tissues; Training; Training and Education; Translational Repression; United States National Institutes of Health; Universities; Work; cell motility; direct application; experience; instrument; instrumentation; neuronal cell body; programs; public health relevance; research study; sexual dimorphism; therapeutic target

DESCRIPTION (provided by applicant): Developmental biologists from several departments throughout Johns Hopkins University are submitting this application to acquire a COPAS Select Embryo Sorter for use in research and education in studies of Drosophila melanogaster and Caenorhabditis elegans. Historically, these organisms have had a significant impact on our understanding of human biology; a major experimental limitation, however, has been large-scale sorting of organisms of distinct genotypes. Although manual sorting techniques are successfully employed to obtain small samples, this procedure is too inefficient to isolate enough tissue for many applications, including microarray analyses, quantitative RT-PCR and biochemistry, or to use for large-scale genetic screens. Thus, we are applying for funds through the NIH Shared Instrumentation Grant Program (S10) to purchase, maintain and train individuals to use the COPAS Select Embryo Sorter from Union Biometrica. This is the only commercially available instrument of this kind. We have assembled a group of five major users, five minor users and a collaborator from across Johns Hopkins University who work in areas of developmental, molecular and computational biology, and whose research would be substantially enriched by the acquisition of this instrument. The users will focus on questions related to (1) deciphering the transcriptional networks controlling organ morphogenesis and physiology, (2) discovering how sexual dimorphism is controlled in both the germline and soma, (3) learning how germ cells are specified, maintained and, ultimately, differentiate (4) elucidating the signaling pathways controlling axonal targeting and sensory signaling, (5) understanding the cellular and molecular components mediating directed cell migration, (6) revealing the molecular constituents and events controlling myoblast fusion, and (7) deciphering the mechanisms of microRNA-mediated translational repression. This instrument will allow each user to carry out experiments that would otherwise be impossible and will also provide a mechanism for the cross-fertilization of ideas and the augmentation of research training and education. The embryo sorter will be housed in common space in the Cell Biology Department, adjacent to the laboratory of the PI on this grant. This site can be easily accessed by all of the users and is near several individuals who have experience using an earlier version of the instrument at other universities. Moreover, a research specialist in the PI's laboratory will be in charge of routine maintenance and training all new users. Public Health Relevance: Model organisms have become increasingly important in revealing the molecular and cellular mechanisms underlying normal development and disease. The studies included in this application are directed toward understanding key developmental events in organ and germ cell specification and differentiation in two experimental model organisms: the fruit fly Drosophila melanogaster and the nematode worm, Caenorhabditis elegans. Molecules and mechanisms discovered through these studies will provide therapeutic targets for treatment of diseases of the vasculature and kidneys, for reestablishing functional neuronal connections after traumatic injury, and for organ replacement therapy.

描述（由申请人提供）：约翰霍普金斯大学多个部门的发展生物学家正在提交此申请，以获取COPAS精选的胚胎分隔器，用于研究和教育，用于研究果蝇Melanogaster和Caenorhabditis elemans。从历史上看，这些生物对我们对人类生物学的理解产生了重大影响。然而，一个主要的实验限制是对不同基因型的生物的大规模分类。尽管手动分选技术已成功地用于获取少量样品，但此过程过于效率低，无法将足够的组织分离用于许多应用，包括微阵列分析，定量RT-PCR和生物化学，或用于大规模遗传筛查。因此，我们正在通过NIH共享仪器赠款计划（S10）申请资金，以购买，维护和培训个人使用Union Biometrica的COPAS选择胚胎分散者。这是此类商业上唯一可用的工具。我们组建了一组由约翰霍普金斯大学（Johns Hopkins University）的五个主要用户，五个未成年用户和一名合作者组成的，他们从事发展，分子和计算生物学领域的工作，并且通过获取该工具的获取将大大实现其研究。用户将重点关注与（1）解释控制器官形态发生和生理学的转录网络相关的问题，（2）发现在种系和SOMA中如何控制性二态性，（3）（3）学习如何指定，维护生殖细胞，并最终划分（最终），以区分（4）授予信号传播的信号式轴突靶标和感觉式信号（5）（5）（5）定向细胞迁移，（6）揭示了控制肌细胞融合的分子成分和事件，以及（7）解密microRNA介导的翻译抑制的机制。该工具将允许每个用户进行原本是不可能的实验，还将为思想交叉施用和研究培训和教育的增强提供一种机制。胚胎分系程序将安置在细胞生物学部门的共同空间中，该赠款与PI实验室相邻。所有用户都可以轻松地访问此站点，并且附近有几个在其他大学使用该乐器的经验的人。此外，PI实验室的研究专家将负责常规维护和培训所有新用户。公共卫生相关性：模型生物在揭示正常发育和疾病的分子和细胞机制方面变得越来越重要。本应用中包括的研究旨在理解两种实验模型生物体中器官和生殖细胞规范和分化的关键发育事件：果蝇果蝇Melanogaster和线虫蠕虫秀丽隐杆线虫。通过这些研究发现的分子和机制将为治疗脉管系统和肾脏疾病的治疗靶标提供治疗靶标，以便在创伤性损伤后重建功能性神经元连接以及器官替换治疗。