Realtime Sensing of Wound Microenvironment

实时感知伤口微环境

• 批准号: 7789640
• 负责人: Alexander Nevin Cartwright
• 金额: $ 24.14万
• 依托单位: STATE UNIVERSITY OF NEW YORK AT BUFFALO
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-04-01 至 2013-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7789640
• 关键词: Acute; Affect; Anatomy; Architecture; Area; Binding; Biological; Biological Assay; Biological Markers; Burn injury; Charge; Chemicals; Chronic; Clinical; Collection; Color; Coupled; Development; Devices; Diagnostic; Down-Regulation; Elements; Environment; Equipment; Event; Feedback; Film; Fluorescence; Funding; Goals; Hand; Healed; Health Care Costs; Healthcare; Image; In Vitro; Individual; Injury; Laboratories; Location; Methods; Monitor; Morbidity - disease rate; Nanotechnology; Optics; Outcome; Patient Care; Patients; Physiological; Polymers; Printing; Process; Property; Protein Array; Proteins; Quantum Dots; Research; Research Personnel; Resolution; Role; Science; Semiconductors; Signal Transduction; Site; Skin; Source; Spatial Distribution; Specificity; Structure; System; Systems Analysis; Techniques; Technology; Testing; Time; Tissues; Translating; Work; Wound Healing; base; clinical application; clinically relevant; cost; design; detector; flexibility; healing; hybrid protein; imprint; improved; in vivo; instrument; interest; light emission; lithography; luminescence; metal oxide; nanoparticle; nanostructured; novel; photonics; point of care; polymerization; programs; protein expression; public health relevance; repaired; restoration; sensor; solid state; wound

DESCRIPTION (provided by applicant): We propose to develop a photonic-based sensing layer for the spatial and temporal determination of proteins within the microenvironment of a wound. This program will combine Protein Imprinted Xerogels with Integrated Emission Sites (PIXIES) with photonic bandgap structures to provide a flexible protein sensing film that can be directly integrated into a point-of-care solid state system for wound status determination. These protein sensing films can be directly integrated with Complementary Metal Oxide Semiconductor (CMOS) technologies to provide low-power, portable, hand held systems for clinical applications. The composite sensing films will be fabricated using optical interference lithography combined with pin-printing. The resulting new class of organic PBG-based sensors integrated with our PIXIES technology that will enable real time assessment of biomarkers that are significant in the care of patients with acute and chronic wounds. The first six months of the project will focus on the demonstration of selectivity and sensitivity of a PIXIES array to multiple proteins printed on a flexible polymer substrate, and imaged using a CCD camera, for in-vitro wound assessment. This will be followed by the development of a porous photonic bandgap (PBG) architectures that can be directly integrated with the PIXIES to efficiently collect the protein-concentration-dependent fluorescence emission from the PIXIES elements. These hybrid PIXIES/PBG structures will be validated using in-vitro wound assessment. The enhanced emission collection efficiency of the PIXIES/PBG structures will enable the use of low cost, low gain, CMOS technologies that can enable rapid dissemination of the developed instruments as portable test equipment. Finally, once there is sufficiently enhanced sensitivity to signal changes, i.e., better resolution of analyte concentration for real time wound assessment will be demonstrated. We intend to use the results from this R-21 as the starting point for the development of a handheld point of care device using a CMOS based low power, hand-held analysis system for in-vivo wound analysis through RO-1 sponsored funding. PUBLIC HEALTH RELEVANCE: Real-time wound analysis has the potential to translate to decreased patient morbidity and health care cost in clinical applications. It is estimated that on any given day 60 million people worldwide are being treated for chronic, non-healing wounds and the ability to tailor patient treatment to obtain improved outcomes has been difficult. The ability to analyze the microenvironment of a wound will result in personalized health care for individual patients that should result in significantly improved healing outcomes.

描述（由申请人提供）：我们建议开发一个基于光子的传感层，以用于伤口微环境内蛋白质的空间和时间测定。该程序将将蛋白质印记与集成发射位点（PIX）与光子带隙结构结合起来，以提供柔性蛋白质感应膜，该蛋白传感膜可以直接整合到护理点固态确定的固态固态系统中。这些蛋白质传感膜可以直接与互补的金属氧化物半导体（CMOS）技术直接整合，以提供用于临床应用的低功率，便携式，手持系统。复合传感膜将使用光学印刷形成与针打印机结合制造。由我们的像素技术集成的新型有机PBG传感器，该传感器将实现对急性和慢性伤口患者护理的生物标志物的实时评估。该项目的前六个月将集中于在柔性聚合物底物上印刷的多种蛋白质的选择性和灵敏度的演示，并使用CCD摄像头进行成像，以进行体外伤口评估。随后将开发多孔光子带隙（PBG）结构，该体系可以直接与像素直接集成，以有效地收集蛋白质浓度依赖蛋白质依赖的荧光发射从Pixies元素中。这些杂交像素/PBG结构将使用视频伤口评估进行验证。 PIXIES/PBG结构的发射收集效率增强将使低成本，低增益，CMOS技术能够用作便携式测试设备的快速传播。最后，一旦对信号变化具有足够的增强敏感性，即将证明分析物浓度更好地分辨出实时伤口评估。我们打算使用基于CMOS的低功率，手持分析系统，通过RO-1赞助的资金使用基于CMOS的低功率，手持分析系统来开发手持式护理设备的结果。公共卫生相关性：实时伤口分析有可能转化为临床应用中患者的发病率和医疗保健成本的降低。据估计，在任何给定的一天中，全世界有6000万人都因慢性，非治疗伤口而受到治疗，并且很难量身定制患者治疗以改善结果。分析伤口微环境的能力将为个体患者提供个性化的医疗保健，这应该显着改善愈合结果。

项目成果

CMOS Imaging of Pin-Printed Xerogel-Based Luminescent Sensor Microarrays.

• DOI: 10.1109/jsen.2010.2047497
• 发表时间: 2010-12
• 期刊: IEEE sensors journal
• 影响因子: 4.3
• 作者: Yao L;Yung KY;Khan R;Chodavarapu VP;Bright FV
• 通讯作者: Bright FV

Phase separation at the surface of poly(ethylene oxide)-containing biodegradable poly(L-lactic acid) blends.

含聚环氧乙烷的可生物降解聚（L-乳酸）共混物表面的相分离。

• DOI: 10.1021/la901239v
• 发表时间: 2009
• 期刊: Langmuir : the ACS journal of surfaces and colloids
• 作者: Yu,Jinxiang;Mahoney,ChristineM;Fahey,AlbertJ;HicksJr,WesleyL;Hard,Robert;Bright,FrankV;GardellaJr,JosephA
• 通讯作者: GardellaJr,JosephA

Hybrid oxygen-responsive reflective Bragg grating platforms.

混合氧响应反射布拉格光栅平台。

• DOI: 10.1021/ac2024816
• 发表时间: 2012
• 期刊: Analytical chemistry
• 影响因子: 7.4
• 作者: Yung,KaYi;Xu,Huina;Liu,Ke;Martinez,GreggoryJ;Bright,FrankV;Detty,MichaelR;Cartwright,AlexanderN
• 通讯作者: Cartwright,AlexanderN

Holographic photopolymer linear variable filter with enhanced blue reflection.

• DOI: 10.1021/am405509t
• 发表时间: 2014-03-12
• 期刊: ACS APPLIED MATERIALS & INTERFACES
• 影响因子: 9.5
• 作者: Moein, Tania;Ji, Dengxin;Zeng, Xie;Liu, Ke;Gan, Qiaoqiang;Cartwright, Alexander N.
• 通讯作者: Cartwright, Alexander N.

Contact CMOS imaging of gaseous oxygen sensor array.

• DOI: 10.1016/j.snb.2011.04.074
• 发表时间: 2011-10-01
• 期刊: SENSORS AND ACTUATORS B-CHEMICAL
• 影响因子: 8.4
• 作者: Daivasagaya, Daisy S.;Yao, Lei;Yung, Ka Yi;Hajj-Hassan, Mohamad;Cheung, Maurice C.;Chodavarapu, Vamsy P.;Bright, Frank V.
• 通讯作者: Bright, Frank V.