Identification of small molecule inhibitors of Pseudomonas aeruginosa lipoxygease (pLOXA)-induced ferroptosis

铜绿假单胞菌脂氧酶 (pLOXA) 诱导的铁死亡小分子抑制剂的鉴定

• 批准号: 10691149
• 负责人: Anton Simeonov
• 金额: $ 14.62万
• 依托单位: NATIONAL CENTER FOR ADVANCING TRANSLATIONAL SCIENCES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10691149
• 关键词: Biological Assay; Cells; Cessation of life; Lipoxygenase; Polyunsaturated Fatty Acids; Pseudomonas aeruginosa; Theft; Work; new therapeutic target; pathogenic bacteria; programs; screening; small molecule inhibitor

The bacterial pathogen - Pseudomonas aeruginosa - can oxygenate arachidonic acidphosphatidylethanolamines (AA-PE) in host cells and induce theft-ferroptosis by hijacking the mammalian polyunsaturated fatty acid PUFA-PE and death program. As such, lipoxygenase (pLoxA) may represent a new therapeutic target against this bacterial threat. During this period, the project team worked to optimize a primary screening assay capable of identifying inhibition of Pseudomonas aeruginosa lipoxygease (pLOXA)-induced ferroptosis.

细菌病原体——铜绿假单胞菌——可以氧化宿主细胞中的花生四烯酸磷脂酰乙醇胺（AA-PE），并通过劫持哺乳动物多不饱和脂肪酸PUFA-PE和死亡程序来诱导盗窃铁死亡。因此，脂氧合酶（pLoxA）可能代表针对这种细菌威胁的新治疗靶点。 在此期间，项目团队致力于优化初级筛选试验，该试验能够识别铜绿假单胞菌脂氧酶（pLOXA）诱导的铁死亡的抑制作用。