CENTER FOR HIGH-THROUGHPUT MINIMALLY-INVASIVE DOSIMETRY

高通量微创剂量测定中心

• 批准号: 7924254
• 负责人: DAVID JONATHAN BRENNER
• 金额: $ 40万
• 依托单位: COLUMBIA UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-22 至 2011-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7924254
• 关键词: CENTER; THROUGHPUT; MINIMALLY; INVASIVE; DOSIMETRY

Our overall theme is product development for very high throughput minimally-invasive radiation biodosimetry. After a large-scale radiological event, there will be a major need to assess, within a few days, the radiation doses received by tens of thousands of individuals. By contrast, current "high throughput" biodosimetry can, at best, assess a few hundred individuals / day. We will develop practical minimally-invasive devices to meet this need, capable of assessing tens of thousands of individuals per day. The Consortium represents a multidisciplinary balance between radiation biologists, radiation physicists, radiation chemists, mechanical engineers, software engineers, product development experts, commercial companies in the field, and end users. The three areas that we have identified as having the highest potential for high-throughput biodosimetry are cytogenetics, functional genomics, and metabolomics; each area has its own project, supported by a bioinformatics core, a functional genomics core, a fabrication core and, crucially, a product development core. The three projects are linked in terms of the overall theme, their use of the Core Resources, and also in terms of their shared use of the same sources of human samples - blood/urine/buccal cells from total-body irradiated patients: Project 1: Automated Robotically-Based High-Throughput Radiation Biodosimetry - We will develop a high-throughput biodosimetry device, using purpose-built robotics and advanced high speed, automated image acquisition. Throughput will reach 30,000 samples per day, compared with current throughputs of a few hundred samples per day. Several endpoints (micronuclei and y-H2AX foci) and several tissues (blood lymphocytes, reticuloctyes, and exfoliated cells from urine) can be used. Project 2: Biodosimetry with a Fully Integrated Biochip using Gene Expression Signatures - Exposure to ionizing radiation produces a well-defined dose-dependent signature in terms of changes in gene expression. Our goal is to use such a signature, which will be established through the Functional Genomics Core, to generate a self-contained radiation biodosimeter device, based on a blood finger stick. Project 3: Rapid Non-Invasive Radiation Biodosimetry through Metabolomics - Here the overall aim is to identify and utilize a signature of radiation exposure through metabolomics, in order to develop a very fast non-invasive biodosimetric device based on urine, saliva or sweat. In addition, this Consortium features an extensive radiological teaching program, both in person, and with an innovative E-seminars approach, and a large Pilot Research Program, featuring a novel two-phase review procedure.

我们的总体主题是针对极高的吞吐量最小侵入性辐射生物测量法的产品开发。大规模放射学事件发生后，将在几天内评估成千上万个人接受的辐射剂量。相比之下，当前的“高吞吐量”生物测定法充其量可以评估数百个人 /天。我们将开发实用的微创设备来满足这种需求，能够每天评估成千上万的人。该财团代表了辐射生物学家，辐射物理学家，辐射化学家，机械工程师，软件工程师，产品开发专家，该领域的商业公司和最终用户之间的多学科平衡。我们确定为高通量生物测量法具有最高潜力的三个领域是细胞遗传学，功能基因组学和代谢组学。每个领域都有自己的项目，并由生物信息学核心，功能基因组学核心，制造核心以及至关重要的是产品开发核心的支持。这三个项目与整体主题，它们对核心资源的使用以及它们共同使用相同的人类样本来源 - 血液/尿液/尿液/颊细胞的共同使用：总体辐照患者的血液/尿液/颊细胞： 项目1：使用专用的机器人技术和高级高速，自动化的图像采集，我们将开发一个基于机器人的高通量辐射生物测量法 - 我们将开发高通量生物测量设备。与每天几百个样本的当前吞吐量相比，吞吐量每天将达到30,000个样本。可以使用几个终点（微核和Y-H2AX焦点）和几个组织（血液淋巴细胞，网状细胞和尿液中的去角质细胞）。 项目2：使用基因表达特征完全整合生物芯片的生物测量法 - 暴露于电离辐射会在基因表达的变化方面产生明确定义的剂量依赖性特征。我们的目标是使用这样的签名，该签名将通过功能基因组核心建立，以基于血液手指棒生成一个独立的辐射生物测量表。 项目3：通过代谢组学快速非侵入性辐射生物测量法 - 这里的总体目的是通过代谢组学识别和利用辐射暴露的特征，以开发一种基于尿液，唾液或汗水的非常快速的非侵入性生物测量器。 此外，该联盟还采用了广泛的放射学教学计划，无论是亲自，具有创新的电子序列方法，以及一个大型的试点研究计划，其中包含一种新颖的两阶段审查程序。