Hydrogel Particle-Based microRNA Profiling for Discovery and Cancer Diagnostics

基于水凝胶颗粒的 microRNA 分析用于发现和癌症诊断

• 批准号: 7747007
• 负责人: Patrick S Doyle
• 金额: $ 24.99万
• 依托单位: FIREFLY BIOWORKS, INC.
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-01 至 2010-08-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7747007
• 关键词: Arts; Binding; Biological Assay; Brain; Cancer Detection; Cancer Diagnostics; Cancerous; Clinical; Code; Data; Detection; Diabetes Mellitus; Diagnosis; Diagnostic; Disease; Down-Regulation; Ethylene Glycols; Future; Goals; Heart Diseases; Human; Hydrogels; Intention; Kinetics; Label; Lung; Malignant Neoplasms; Marketing; MicroRNAs; Molecular Profiling; Nature; Nucleotides; Performance; Phase; Phase II Clinical Trials; Placenta; RNA; Reproducibility; Research; Sampling; Scanning; Scheme; Screening procedure; Sensitivity and Specificity; Sequence Homology; Small Business Innovation Research Grant; Solutions; Specificity; Speed; Stream; Structure; System; Targeted Research; Technology; Testing; Thermodynamics; Tissues; anticancer research; base; cost; drug discovery; ethylene glycol; high throughput screening; human tissue; particle; public health relevance; success; tool

DESCRIPTION (provided by applicant): MicroRNA targets have shown enormous potential for understanding, diagnosing, and even treating the world's most prevalent diseases including cancer, heart disease, Alzheimer's, diabetes and many more. Unfortunately, miRNAs are particularly challenging to quantify due to their small size, sequence homology, and wide range of abundance. As such, miRNA profiling is either expensive or low-throughput using today's state-of-the art technologies. To overcome these limitations, a new multiplexing technology built on encoded hydrogel microparticles will be developed for miRNA profiling. Preliminary results suggest that this technology will provide high sensitivity, single-nucleotide specificity, and rapid analysis. The specific aims for Phase I of this project are to (1) develop and assess target labeling schemes for high-performance detection, (2) perform multiplexed miRNA profiling over a diverse set of targets, and (3) demonstrate predictive expression profiling for cancer detection in human-derived samples. This project could have implications in cancer research, drug discovery, and cancer diagnostics. PUBLIC HEALTH RELEVANCE: The aim of this project is to develop a technology capable of profiling across all human microRNA in a manner that is accurate, rapid, and inexpensive. This tool will be enabling for cancer research, drug discovery, and cancer diagnostics.

描述（由申请人提供）：microRNA目标显示出巨大的理解，诊断，甚至治疗世界上最普遍的疾病，包括癌症，心脏病，阿尔茨海默氏症，糖尿病等。不幸的是，由于miRNA的尺寸小，序列同源性和广泛的丰度，量化尤其具有挑战性。因此，使用当今最先进的技术，miRNA分析要么是昂贵的或低通量的。为了克服这些局限性，将开发建立在编码水凝胶微粒上的新的多路复用技术，以用于miRNA分析。初步结果表明，该技术将提供高灵敏度，单核苷酸特异性和快速分析。该项目I期第一阶段的具体目的是（1）开发和评估目标标签方案以进行高性能检测，（2）对各种目标进行多重miRNA分析，（3）在人类衍生样品中证明了对癌症检测的预测表达分析。该项目可能对癌症研究，药物发现和癌症诊断有影响。 公共卫生相关性：该项目的目的是开发一种能够以准确，快速和廉价的方式在所有人类microRNA上进行分析的技术。该工具将用于癌症研究，药物发现和癌症诊断。