Multiplexed Encoded Particle PCR for Rapid Panel Diagnosis of Sexually-Transmitte

用于性传播疾病快速组合诊断的多重编码粒子 PCR

• 批准号: 7803524
• 负责人: Patrick S Doyle
• 金额: $ 17.64万
• 依托单位: FIREFLY BIOWORKS, INC.
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-03-05 至 2010-09-04
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7803524
• 关键词: Adolescent; Bacteriophage lambda; Binding; Biological Assay; Chlamydia trachomatis; Clinical; Code; DNA; Detection; Diagnosis; Disease; Enzyme Immunoassay; Epidemic; Ethylene Glycols; Genomics; Gonorrhea; High temperature of physical object; Human; Hydrogels; Infection; Infectious Agent; Kinetics; Length; Lung diseases; Methods; Nature; Neisseria gonorrhoeae; Nucleic Acid Amplification Tests; Nucleotides; Phase; Physicians; Physiological; Reaction; Research; Sampling; Scanning; Screening procedure; Sensitivity and Specificity; Sexually Transmitted Diseases; Signal Transduction; Simulate; Solutions; Specificity; Specimen; Swab; System; Technology; Testing; Thermodynamics; Time; Tube; Urine; Viral Load result; Woman; base; charge coupled device camera; cost; design; ethylene glycol; fluorophore; internal control; men; particle; pathogen; phase 2 study; public health relevance; success; tool

DESCRIPTION (provided by applicant): Sexually-transmitted infections are an epidemic, particularly among adolescents, that could be managed with routine diagnosis and screening. Currently not practical due to cost constraints, there is a need for rapid and reliable assays capable of panel-testing for multiple infections. To this end, we will develop Multiplexed Encoded Particle PCR (mEP-PCR) assays to accurately and rapidly diagnose multiple sexually transmitted diseases (STDs) with a cost-effective, "one- pot" test. The specific aims for Phase I of this project are to (1) identify three multiplex PCR targets for N. gonorrhoeae, C. trachomatis, and positive control genomic targets, (2) optimize a "one-pot" assay where PCR and hybridization are carried out in the same tube, and (3) demonstrate simultaneous reproducible screening for N. gonorrhoeae and C. trachomatis in a single simulated specimen. This project could also be extended to impact screening for respiratory disease, diarrheal disease, and bioterror agents. PUBLIC HEALTH RELEVANCE: The aim of this project is to develop a technology capable of detecting multiple sexually transmitted infections (STIs) in a single physiological sample with high sensitivity and specificity at a very low cost. This tool will be enabling for rapid, cost-effective diagnosis of STIs.

描述（由申请人提供）：性传播感染是一种流行病，尤其是在青少年中，可以通过常规诊断和筛查来管理。目前由于成本限制而不实用，需要快速可靠的测定法，能够进行多种感染的面板测试。为此，我们将开发多路复用的编码粒子PCR（MEP-PCR）测定法，以准确，迅速诊断多种性传播疾病（STD），并具有具有成本效益的“一锅”测试。 The specific aims for Phase I of this project are to (1) identify three multiplex PCR targets for N. gonorrhoeae, C. trachomatis, and positive control genomic targets, (2) optimize a "one-pot" assay where PCR and hybridization are carried out in the same tube, and (3) demonstrate simultaneous reproducible screening for N. gonorrhoeae and C. trachomatis in a single simulated标本。该项目也可以扩展到影响筛查呼吸道疾病，腹泻病和生物疾病剂的筛查。 公共卫生相关性：该项目的目的是开发一种能够在单个生理样本中以高敏感性和特异性以非常低的成本检测多种性传播感染（STI）的技术。该工具将有助于快速，具有成本效益的性传播感染诊断。