Nanoparticle-Hydrogel Strips for Detection of Staphylococcal Enterotoxins

用于检测葡萄球菌肠毒素的纳米颗粒水凝胶条

• 批准号: 7800171
• 负责人: Chuanmin Ruan
• 金额: $ 10万
• 依托单位: SENMATER TECHNOLOGY, LLC
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-20 至 2011-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7800171
• 关键词: Nanoparticle; Hydrogel; Strips; Detection; Staphylococcal

DESCRIPTION (provided by applicant): Rapid, simple and reliable detection of staphylococcal enterotoxin B (SEB) in food products is a crucial step to ensure food safety and prevent food intoxication and gastrointestinal diseases. Current SEB detection is enzyme-linked immunosorbent assay (ELISA), reverse phase latex agglutination and reverse transcription polymerase chain reaction (RT-PCR). In general, these techniques are either time-consuming or labor intensive because of sample incubation, sample pretreatment, tedious washings and extraction of nucleic acid. In this STTR proposal, we are to demonstrate the feasibility to develop a 'pH-test strip like' product for rapid detection of SEB based on photonic crystal silica nanoparticle-hydrogel. We will, for the first time, incorporate SEB specific antibody into SiO2 colloidal photonic crystal array to form a strip that is sensitively responsive to SEB in solutions. In Phase I, the monodisperse silica nanoparticles will be synthesized and the correspondent colloidal photonic crystal array will be fabricated. In Phase II we will expend the concept to other toxins produced by staphylococcus aureus and develop a series of sensitive, reliable and user-friendly commercial products. PUBLIC HEALTH RELEVANCE: The proposed project has practical applications in toxin diagnostics and is intended to improve the safety and security of the nation's biodefense agent and food supply. The objective of this project is to develop an innovative strip technology for rapid detection of Staphylococcal enterotoxins which is listed in NIAID category A, B and C priority pathogens and toxins. The successful outcome of this research should provide direct economic benefits to food and diagnostics industries and the whole society in terms of biosafety.

描述（由申请人提供）：食品中快速，简单和可靠的检测对葡萄球菌肠毒素B（SEB）是确保食品安全并防止食品中毒和胃肠道疾病的关键步骤。当前SEB检测是酶联免疫吸附测定（ELISA），反相乳胶凝集和逆转录聚合酶链反应（RT-PCR）。通常，由于样品孵育，样品预处理，乏味的洗涤和核酸提取，这些技术要么是耗时的或劳动量的。在此STTR提案中，我们将证明开发基于光子晶体二氧化硅纳米粒子 - 凝胶的SEB的“ pH检测条”产物的可行性。我们将首次将SEB特异性抗体掺入SIO2胶体光子晶体阵列中，形成对溶液中SEB敏感的敏感的带。在第一阶段，将合成单分散二氧化硅纳米颗粒，并制造通讯胶体光子晶体阵列。在第二阶段，我们将把这一概念花费给金黄色葡萄球菌生产的其他毒素，并开发一系列敏感，可靠和用户友好的商业产品。 公共卫生相关性：拟议的项目在毒素诊断方面有实际应用，旨在改善国家生物探险剂和食品供应的安全和保障。该项目的目的是开发一种创新的带状技术，用于快速检测葡萄球菌肠毒素，该葡萄球菌肠毒素在NIAID类别A，B和C优先病原体和毒素中列出。这项研究的成功结果应在生物安全方面为粮食和诊断行业以及整个社会提供直接的经济利益。