Gene Expression During Embryonic Development Of Xenopus Laevis

非洲爪蟾胚胎发育过程中的基因表达

基本信息

批准号：
7734711
负责人：
Igor B Dawid
金额：
$ 81.24万
依托单位：
EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH & HUMAN DEVELOPMENT
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/7734711
关键词：
Affect Biological Models DNA Microarray Chip DNA Microarray format Defect Development Dissection Embryo Embryonic Development Failure Family Fibroblast Growth Factor Gene Expression Gene Expression Profile Genes Genetic Genetic Epistasis Guanine Nucleotide Exchange Factors Injection of therapeutic agent Integral Membrane Protein Laboratories Lead Leucine Microarray Analysis Molecular Molecular Genetics Monomeric GTP-Binding Proteins Movement Names Neural Crest Neural Tube Closure Pathway interactions Pattern Peripheral Nervous System Pigmentation physiologic function Population Positioning Attribute Proteins RNA Rana Research Project Grants Role Shapes Signal Pathway Signal Transduction Skeleton Systems Analysis Techniques Tissue Transplantation Tissues Vertebrates Xenopus Xenopus laevis base cell growth regulation cell motility craniofacial functional genomics gene discovery improved interest malformation neuroregulation novel research study rho

项目摘要

This laboratory is studying the molecular basis of embryogenesis in Xenopus laevis. This project utilized DNA microarray technology for gene discovery in the early embryo, with the aim to obtain information on gene expression patterns in development and to lead to improved understanding of the molecular basis of normal embryogenesis. This approach allowed the identification of a protein in the family of guanine nucleotide exchange factors named WGEF that has a role in the Wnt-PCP pathway which regulates convergent extension movements in Xenopus. These cell movements are required in the establishment of body shape, and deficits in these movements can lead to malformations such a neural tube closure defects (NTDs). We have characterized the Rho-GEF we identified as a component of the Wnt-PCP signaling pathway that interacts with the known pathway components Dishevelled and Daam-1. The role and specific position of WGEF in the genetic hierarchy of the control of gastrulation movements has been determined. DNA microarray technology also led to the identification of a leucine-rich transmembrane protein named Lrig3 that we showed to be required for the formation of the neural crest. Experiments in the whole embryo and in explants induced to differentiate into neural crest have shown that the novel protein affects multiple signaling pathways in the embryo, in particular modulates the function of the Fibroblast Growth factor pathway. Epistasis experiments have been carried out to identify the position of the leucine-rich factor in the hierarchy of control of neural crest specification.

该实验室正在研究爪诺邦Laevis中胚胎发生的分子基础。该项目利用DNA微阵列技术在早期胚胎中发现基因发现，目的是获取有关发育中基因表达模式的信息，并提高人们对正常胚胎发生的分子基础的理解。这种方法允许在名为WGEF的鸟嘌呤核苷酸交换因子家族中鉴定蛋白质，该蛋白在Wnt-PCP途径中起作用，该途径调节Xenopus中的收敛延伸运动。这些细胞运动是在体形的建立中需要的，这些运动的缺陷可能导致畸形，例如神经管闭合缺陷（NTD）。我们已经表征了Rho-gef，我们确定为Wnt-PCP信号通路的一个组成部分，该途径与已知途径成分散乱和DAAM-1相互作用。已经确定了WGEF在控制胃运动的遗传层次结构中的作用和特定位置。 DNA微阵列技术还导致鉴定出富含亮氨酸的跨膜蛋白LRIG3，我们证明这是神经rest形成所必需的。在整个胚胎和外植体中的实验是分化为神经rest的，这表明这种新蛋白会影响胚胎中的多个信号通路，特别是调节成纤维细胞生长因子途径的功能。已经进行了上位实验，以确定富含亮氨酸因子在控制神经rest规范层次结构中的位置。

项目成果

期刊论文数量（26）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Cloning and characterization of zebrafish CTCF: Developmental expression patterns, regulation of the promoter region, and evolutionary aspects of gene organization.

斑马鱼 CTCF 的克隆和表征：发育表达模式、启动子区域的调节以及基因组织的进化方面。

DOI：
10.1016/j.gene.2006.01.036
发表时间：
2006
期刊：
Gene
影响因子：
3.5
作者：
Pugacheva,ElenaM;Kwon,Yoo-Wook;Hukriede,NeilA;Pack,Svetlana;Flanagan,PatrickT;Ahn,Jong-Chan;Park,JeongAe;Choi,Kyu-Sil;Kim,Kyu-Won;Loukinov,Dmitri;Dawid,IgorB;Lobanenkov,VictorV
通讯作者：
Lobanenkov,VictorV

Organizing the vertebrate embryo--a balance of induction and competence.