Gene Expression During Embryonic Development Of Xenopus

非洲爪蟾胚胎发育过程中的基因表达

• 批准号: 6508750
• 负责人: Igor B Dawid
• 金额: --
• 依托单位: EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH & HUMAN DEVELOPMENT
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6508750
• 关键词: Xenopus; antisense nucleic acid; biological signal transduction; biomarker; developmental genetics; early embryonic stage; embryogenesis; gene induction /repression; nonmammalian vertebrate embryology; transcription factor; zebrafish

This laboratory has studied the molecular basis of embryogenesis in Xenopus laevis and the zebrafish, with special emphasis on axis determination and pattern formation. These events are thought to be controlled by cell-to-cell signaling and by the spatially and temporally regulated action of transcription factors. The Xlim-1 gene encodes a LIM-homeodomain protein that has been shown to be involved in the functions of the Spemann organizer in neural induction and mesoderm patterning. This gene is also important in kidney formation. The function of Xlim-1 is being studied by application of an oligonucleotide antisense strategy. Modified antisense oligonucleotides that are stable in the embryo have proven valuable tools for the analysis of Xlim-1 function during gastrulation. A screen for developmentally regulated genes is being conducted in zebrafish. By screening for genes with restricted expression patterns, valuable information is collected about gene control of embryogenesis. One gene isolated in this approach, named iro3, has been shown to have a role in organizer function in the gastrula. A zinc finger transcription factor isolated from zebrafish named biklf has been shown to constitute one of the earliest markers for blood formation and to be involved in erythroid cell differentiation. In addition, a genetic screen has been initiated that uses a chemical mutagen expected to generate deletions in the zebrafish genome. Such lesions are expected to be very useful in the genetic and functional characterization of zebrafish genes.

该实验室研究了爪蟾和斑马鱼中胚胎发生的分子基础，并特别强调了轴确定和模式形成。这些事件被认为是通过细胞到细胞信号传导以及在空间和时间调节转录因子的作用来控制的。 XLIM-1基因编码一种lim瘤蛋白，该蛋白已被证明参与了Spemann组织者在神经诱导和中胚层构图中的功能。该基因在肾脏形成中也很重要。通过应用寡核苷酸反义策略来研究XLIM-1的功能。在胚胎中稳定的改良反义寡核苷酸已证明有价值的工具可在胃肠道过程中分析XLIM-1功能。 在斑马鱼中正在进行开发调节基因的屏幕。通过筛选具有受限表达模式的基因，收集了有关胚胎发生基因控制的有价值信息。在这种方法中分离的一种基因，称为IRO3，已被证明在胃中的组织者功能中起作用。已证明从斑马鱼中分离出的锌指转录因子已被证明构成了血液形成的最早标记之一，并参与了红细胞细胞分化。此外，已经启动了一种遗传筛选，该遗传筛选使用预期在斑马鱼基因组中产生缺失的化学诱变剂。预计此类病变在斑马鱼基因的遗传和功能表征中非常有用。