Investigation of the mitochondrial function in GNAS mutant neoplasms

GNAS 突变肿瘤中线粒体功能的研究

• 批准号: 10605302
• 负责人: Krushna Chandra Patra
• 金额: $ 14.22万
• 依托单位: UNIVERSITY OF CINCINNATI
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-05-01 至 2024-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10605302
• 关键词: Acetyl Coenzyme A; Address; Amino Acid Metabolism Pathway; Amino Acids; Biological Assay; Biological Models; Branched-Chain Amino Acids; Cells; Citric Acid Cycle; Coupled; Cyclic AMP; Cyclic AMP-Dependent Protein Kinases; Data; Dependence; Development; Equilibrium; Exhibits; FADH2; Foundations; GTP-Binding Proteins; Genetic; Genetically Engineered Mouse; Gnas protein; Goals; Growth; Human; Implant; Investigation; Lead; Lipids; Maintenance; Malignant Neoplasms; Malignant neoplasm of gastrointestinal tract; Malignant neoplasm of pancreas; Maps; Mediating; Metabolic; Metabolic Pathway; Metabolism; Microscopy; Mitochondria; Mitochondrial Proteins; Modeling; Mus; Mutation; NADH; Neoplasms; Normal Cell; Normal tissue morphology; Nutrient; Oncogenes; Oncogenic; Organoids; Pancreatic Ductal Adenocarcinoma; Pathway interactions; Patients; Physiological Processes; Process; Production; Protein Subunits; Proteins; Proteome; Proteomics; Publishing; Reaction; Recurrence; Regulation; Role; Series; Signal Pathway; Signal Transduction; Solid; Structure; Testing; Therapeutic; Therapeutically Targetable; Up-Regulation; aerobic glycolysis; amino acid metabolism; branched chain fatty acid; cancer cell; cancer subtypes; cancer type; experimental study; fatty acid oxidation; gain of function mutation; innovation; metabolomics; mitochondrial metabolism; mutant; neoplastic cell; new therapeutic target; pancreatic tumorigenesis; patient population; pharmacologic; preclinical study; programs; protein expression; salt-inducible kinase; subcutaneous; tumor; tumor growth; tumor initiation; tumorigenesis

Emerging evidences pointing out the requirement of mitochondrial function in the oncogenesis processes, but how the mitochondrial state is changed in different set of oncogenic mutations is largely obscure. Changes in mitochondrial metabolism and function are results of mitochondrial dynamics (fission/fusion) and remodeled mitochondrial proteome. cAMP/PKA pathway has conserved roles in the regulation of mitochondrial function and dynamics in various physiological processes. The goal of this proposal is to comprehensively analyze the mitochondrial function and state in a subset of pancreatic cancer those harbor gain-of-function mutations of GNAS (~60%). In a recent study I discovered mutant GNAS activates cAMP/PKA pathway that leads to inactivation salt-inducible-kinases (SIKs) and rewiring metabolic processes, a major mechanism required for tumor growth of these tumors. The global multiplex proteomics data revealed selective remodeling of cellular proteome by mutant GNAS activation. Specifically there is enrichment proteins of fatty acid oxidation (FAO) and branched chain amino acid (BCAA) pathways, which are compartmentalized in the mitochondria. Based on these preliminary data and the pivotal role of cAMP/PKA in controlling mitochondrial dynamics and metabolism, I hypothesize that mitochondria has profound roles in the oncogenesis process by mutant GNAS. Building on the solid foundation, this K22 proposal aims to map the GNAS-PKA-SIK mediated changes in mitochondrial dynamics and proteomic landscape. It will test the hypothesis that the changes in dynamics and proteome remodeling lead to altered FAO and BCAA pathway function, which are required for growth of GNAS mutant tumors. Using state-of-art organellar purification, proteomics, metabolic tracing and coupled with functional assays in pancreatic cancer organoids; I seek to identify the key function of mitochondria in GNAS mutant cancer. Results from these studies will give unparalleled understanding of oncogenic cAMP signaling in cancer and may lead to development of new-targeted therapies that can be used in genetically-defined patient populations.

新兴的证据指出了在肿瘤发生过程中线粒体功能的要求，但 线粒体状态如何在不同的致癌突变中更改很大程度上是晦涩的。变更 线粒体代谢和功能是线粒体动力学（裂变/融合）的结果，并重塑 线粒体蛋白质组。 CAMP/PKA途径在线粒体功能的调节中具有保守的作用 和各种生理过程中的动态。该建议的目的是全面分析 线粒体功能和状态在胰腺癌的一部分中 GNA（〜60％）。在最近的一项研究中，我发现突变GNA激活了CAMP/PKA途径，这导致 灭活盐诱导酶（Siks）和重新布线代谢过程，这是需要的主要机制 这些肿瘤的肿瘤生长。全局多重蛋白质组学数据揭示了细胞的选择性重塑 突变GNA激活的蛋白质组。具体而言，有脂肪酸氧化（FAO）的富集蛋白 和分支链氨基酸（BCAA）途径，这些途径在线粒体中被划分。基于 关于这些初步数据以及CAMP/PKA在控制线粒体动力学和 代谢，我假设线粒体在突变GNA的肿瘤发生过程中具有重要作用。 这项K22提案以固体基础为基础，旨在绘制GNAS-PKA-SIK介导的变化 线粒体动力学和蛋白质组学景观。它将测试动态变化和动态变化的假设 蛋白质组重塑导致粮农组织和BCAA途径功能改变，这是GNA生长所必需的 突变肿瘤。使用最先进的细胞器纯化，蛋白质组学，代谢追踪并与 胰腺癌器官的功能测定；我试图确定线粒体在GNA中的关键功能 突变癌。这些研究的结果将使人们无与伦比地理解过性cAMP信号传导 癌症，可能导致可用于遗传定义患者的新靶向疗法的发展 人群。