Effects of binge alcohol drinking on synaptic computing in the nucleus accumbens

酗酒对伏隔核突触计算的影响

• 批准号: 10392419
• 负责人: Gilles E MARTIN
• 金额: $ 44.47万
• 依托单位: UNIV OF MASSACHUSETTS MED SCH WORCESTER
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-05-01 至 2025-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10392419
• 关键词: Acute; Adolescence; Adolescent; Adult; Affect; Affective; Age; Alcohol consumption; Alcohol dependence; Alcohols; Amygdaloid structure; Animals; Aversive Stimulus; Behavior; Brain; Chronic; Cognitive; Communication; Consumption; Data; Decision Making; Electrophysiology (science); Emotional; Equilibrium; Ethanol; Glutamates; Goals; Impulsivity; In Vitro; Individual; Judgment; Laboratories; Laser Scanning Microscopy; Link; Mediating; Modeling; Mus; Neurons; Nucleus Accumbens; Opsin; Pathway interactions; Pharmaceutical Preparations; Pharmacological Treatment; Phase; Play; Prefrontal Cortex; Process; Property; Prosencephalon; Research Support; Rewards; Role; Signal Transduction; Slice; Structure; Synapses; System; Techniques; Testing; Transgenic Mice; Translating; Wild Type Mouse; age related; alcohol effect; alcohol exposure; base; binge drinking; biophysical techniques; cognitive computing; drinking; drug of abuse; executive function; experimental study; hippocampal pyramidal neuron; improved; insight; mouse genetics; nervous system disorder; neural circuit; neurochemistry; neuronal excitability; novel; optogenetics; patch clamp; theories; two-photon; vulnerable adolescent

The ambition of this proposal is to identify the cellular underpinnings of the early transition between casual to sustain drinking using drinking-in-the-dark model. We propose to employ a variety of complementary approaches, including mouse genetics, optogenetics, behavior and biophysical approaches to test the overarching hypothesis that computing of the synaptic strength of cortical and amygdala inputs (a phenomenon also known as synaptic gating) is a mechanism enabling nucleus accumbens medium spiny neurons to integrate cognitive and emotional information, and is modulated by alcohol exposure to control consumption. We postulate that this cellular mechanism contributes to the vulnerability of adolescents to binge drinking.

该提案的目标是确定早期转变的细胞基础 随意饮酒与维持饮酒之间采用黑暗饮酒模式。我们建议聘用 各种补充方法，包括小鼠遗传学、光遗传学、 行为和生物物理方法来检验计算的总体假设 皮质和杏仁核输入的突触强度（这种现象也称为 突触门控）是一种使伏隔核中型多棘神经元能够 整合认知和情感信息，并受到酒精暴露的调节 控制消费。我们假设这种细胞机制有助于 青少年容易酗酒。