In vivo and in vitro metabolic flux analysis in preclinical mouse models

临床前小鼠模型的体内和体外代谢流分析

• 批准号: 10926284
• 负责人: Mioara Larion
• 金额: $ 28.5万
• 依托单位: DIVISION OF BASIC SCIENCES - NCI
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10926284
• 关键词: Brain; Clinical; Disease Progression; Glucose; Glutamine; Image; In Vitro; Injections; Label; Laboratories; Magnetic Resonance Imaging; Measures; Metabolic; Mus; Normal Cell; Pathway interactions; Protocols documentation; Source; cohort; experimental study; in vivo; mouse model; pre-clinical; tumor

Our mice protocol has been approved for the next 3 years. We plan to use a cohort of mice for the metabolic flux analysis and correlate with in in vitro studies. We are in the initial stages of this project and therefore do not have significant result yet. Mice that developed mature tumors will be transported from building 37 to building 10 MIF initially for different field imaging using 1H-MRS and 13C-MRS then, to Dr. Murali Cherukuri's laboratory, where the injection of precursors will take place right before the hyperpolarized MRI experiment. In between experiments, we will keep the mice in the allocated cage in building 10. We will inject the mice with the 13C precursors in order to probe different pathways then immediately start the imaging experiment.

我们的小鼠方案已获得未来 3 年的批准。我们计划使用一组小鼠进行代谢流分析并与体外研究相关联。我们正处于该项目的初始阶段，因此尚未取得重大成果。产生成熟肿瘤的小鼠将被从 37 号楼运送到 10 号楼 MIF，最初使用 1H-MRS 和 13C-MRS 进行不同的视野成像，然后转移到 Murali Cherukuri 博士的实验室，在超极化 MRI 之前注射前体细胞实验。在实验之间，我们将把小鼠关在10号楼分配的笼子里。我们会给小鼠注射13C前体，以探测不同的途径，然后立即开始成像实验。