Resource and Service Center

资源与服务中心

• 批准号: 10471392
• 负责人: John Richard Seavitt
• 金额: $ 36.87万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-15 至 2025-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10471392
• 关键词: Address; Affect; Alleles; Animal Model; Animal Testing; Animals; Applications Grants; Archives; Area; Bioinformatics; Clinic; Communities; Community Outreach; Data Analyses; Disease; Disease model; Drosophila genus; Ensure; Environment; Fee-for-Service Plans; Genetic Engineering; Genetic Identity; Genetic Translation; Genetic Variation; Goals; Health Services Accessibility; Heart; Infrastructure; Knockout Mice; Leadership; Link; Macaca mulatta; Maintenance; Methods; Modeling; Mus; Patient risk; Patients; Phenotype; Preservation Technique; Procedures; Process; Production; Publishing; Reproducibility; Research; Research Design; Research Personnel; Resources; Rice; Risk Assessment; Sample Size; Services; Site; Standardization; Statistical Methods; Structure; Technology; Text; Time; animal resource; cost; fly; gene discovery; genome editing; genome integrity; human disease; human genome sequencing; instrumentation; meetings; network models; news; operation; precision medicine; preclinical study; preservation; programs; public repository; quality assurance; screening; social media; somatic cell gene editing; web site

ABSTRACT Poor reproducibility of published research employing model organisms and lack of transparency of study designs have become areas of concern for the scientific community. These issues can profoundly affect the interpretation of preclinical studies and must be addressed as genome editing technologies permit rapid modeling of patient- specific, disease-associated genetic variation and translation to the clinic in the forms of patient risk assessment, management, and treatment. At the heart of reproducibility issues are the model organism themselves. Stability and identity of the genetic background, genome integrity, and specifics of genetically engineered alleles often vary between studies due to use of similar but independently developed models or differences in the quality of model maintenance. Centralization and standardization of high-quality production and maintenance of animal models can begin to address issues with study reproducibility and transparency and has been highly successful for our existing animal modeling programs. The Resource and Service Section of the BCM Center for Precision Medicine Modeling will build on existing expertise and infrastructures of the Undiagnosed Diseases Network Model Organism Screening Center Drosophila Core at BCM, the BCM Knockout Mouse Production and Phenotyping site, the BCM-Rice Small Animal Testing Center for the Somatic Cell Genome Editing Program, and a large rhesus macaque sequence resource in the Human Genome Sequencing Center to (1) acquire, characterize, and maintain high-quality fly and mouse resources, (2) preserve and distribute fly and mouse resources, (3) coordinate Center fee-for-service activities for biomedical researchers, (4) communicate the availability and quality of Center resources and services, and (5) enact procedures to evaluate Section operations.

抽象的 使用模式生物的已发表研究的重复性差且研究设计缺乏透明度 已成为科学界关注的领域。这些问题都会深刻影响解释 临床前研究的问题必须得到解决，因为基因组编辑技术允许对患者进行快速建模 特定的、与疾病相关的遗传变异，并以患者风险评估的形式转化为临床， 管理、治疗。再现性问题的核心是模式生物本身。稳定 遗传背景的身份、基因组完整性以及基因工程等位基因的细节通常 由于使用相似但独立开发的模型或研究质量的差异，研究之间存在差异 模型维护。动物高质量生产和饲养的集中化和标准化 模型可以开始解决研究可重复性和透明度的问题，并且已经取得了巨大成功 对于我们现有的动物建模程序。 BCM精密中心资源与服务部 医学建模将建立在未确诊疾病网络的现有专业知识和基础设施的基础上 BCM 模式生物筛选中心果蝇核心、BCM 基因敲除小鼠生产和 表型分析网站，BCM-Rice 小动物体细胞基因组编辑项目测试中心， 以及人类基因组测序中心的大型恒河猴序列资源，用于 (1) 获取、 描述和维护高质量的苍蝇和小鼠资源，(2) 保存和分布苍蝇和小鼠 资源，(3) 协调中心针对生物医学研究人员的收费服务活动，(4) 传达 中心资源和服务的可用性和质量，以及 (5) 制定评估程序 运营。