MICROTUBULE PROTEINS OF THE VERTEBRATE PHOTORECEPTOR

脊椎动物感光器的微管蛋白

• 批准号: 3426598
• 负责人: DAVID J ASAI
• 金额: $ 3.73万
• 依托单位: PURDUE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-08-01 至 1991-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3426598
• 关键词: binding proteins; cell biology; cell type; cilium; cow; cytoskeletal proteins; density gradient ultracentrifugation; dynein ATPase; hybridomas; microtubule associated protein; monoclonal antibody; tissue /cell culture; visual photoreceptor

The microtubule cytoskeleton is an important component of the vertebrate photoreceptor. It contributes to the structural integrity of the photoreceptor cell, it mediates the vectorial trafficking of outer segment precursors via the connecting cilium, and it may be a critical target for proteolysis during retinal degeneration. In order to understand the function of the microtubule cytoskeleton, it is necessary to discover the complete repertoire of microtubule-associated proteins (MAPs) and to elucidate their structures. The objective of this project is to identify and characterize photoreceptor MAPs. It is the hypothesis of this project that there exist heretofore unidentified photoreceptor MAPs. This project will employ a directed strategy based upon the unique ability of all MAPs to bind to microtubules with significant affinity. The photoreceptor MAPs will be characterized by comparing them to known MAPs (originally characterized from non-retinal sources) and by testing whether any of the MAPs displays characteristics common to microtubule motor proteins (dynein or kinesin). Monoclonal antibodies will be raised and characterized against the novel photoreceptor MAPs. These antibodies will be utilized to localize each MAP in the retina. The project is a first-time entry into the field of vision research by a laboratory with extensive experience in the identification and characterization of MAPs structural MAPs and microtubule motors) in other systems. The proposal outlines an innovative venture because it will apply a novel procedure to attempt to discover heretofore unidentified photoreceptor MAPs. If interesting MAPs are discovered, then much work beyond the single year of the Pilot Project will be required to characterize them and to determine their function in normal and diseased tissue, and the results obtained during the Pilot Project will serve as the basis for future, more extensive studies. On the other hand, if the strategy reveals no novel MAPs, then his would also be an important finding which should be shared with others who might contemplate similar approaches in their study of the photoreceptor.

微管细胞骨架是脊椎动物的重要组成部分 感光器。它有助于 感光细胞，它介导了外部段的矢量运输 通过连接纤毛的前体，它可能是 视网膜变性过程中的蛋白水解。为了了解 微管细胞骨架的功能，有必要发现 微管相关蛋白质（地图）的完整曲目和 阐明其结构。该项目的目的是确定 并表征光感受器图。这是这个项目的假设 存在迄今未识别的光感受器图。这个项目 将根据所有地图的独特能力采用定向策略 与具有显着亲和力的微管结合。光感受器图 将其特征是将它们与已知地图进行比较（最初 通过非视网膜来源的特征）以及测试是否有任何 地图显示了微管运动蛋白（动力蛋白）常见的特征 或动力素）。单克隆抗体将被抬高并针对 新型的光感受器图。这些抗体将用于本地化 视网膜中的每张地图。 该项目是首次进入视力研究领域的 实验室在身份证明方面具有丰富的经验 在其他中表征地图结构地图和微管电动机） 系统。该提案概述了一项创新的企业，因为它将适用 试图发现迄今未知的新型程序 光感受器图。如果发现有趣的地图，那么很多工作 除了飞行员项目的一年之外 表征它们并确定它们在正常和患病中的功能 组织，并在试点项目期间获得的结果将作为 未来的基础，更广泛的研究。另一方面，如果 策略没有揭示任何新的地图，那么他的地图也将是一个重要的发现 应该与可能考虑类似方法的其他人共享 在对感光器的研究中。