CELLULARIZATION IN ASPERGILLUS NIDULANS

构巢曲霉的细胞化

• 批准号: 6180153
• 负责人: DAVID J ASAI
• 金额: $ 24.98万
• 依托单位: PURDUE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-05-01 至 2002-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6180153
• 关键词: Aspergillus; biological signal transduction; cell cycle; cell differentiation; cell growth regulation; cell morphology; cell motility; confocal scanning microscopy; cytogenetics; cytoskeletal proteins; fluorescence microscopy; fungal genetics; fusion gene; gene complementation; gene expression; green fluorescent proteins; histogenesis; immunoprecipitation; microtubules; mitotic spindle apparatus; molecular cloning; orientation; polymerase chain reaction; protein kinase; protein structure function; regulatory gene

The long term goals of this research are to determine how eukaryotic cells orient and position the contractile actin ring during cytokinesis. The precise orientation of cell division planes can impart different fates to daughter cells and is of fundamental importance to all eukaryotic cells. It has long been known that the ailment of the mitotic spindle dictates the axis of cleavage, however the mechanism by which the components of the mitotic spindle, namely the microtubules, communicates this information to the cell is unknown. We are studying the process in the fungus, Aspergillus nidulans. A. nidulans has a rich history as a model genetic organism for studies of mitosis and microtubule-mediated processes. The cells of A. nidulans are divided by crosswalls called septa and the process of septation involves a contractile acting ring whose formation is dependent on microtubules. Mutational analysis has been used to define a collection of genes required for septation. A group of these genes, sepH, sepD and sepG, appear to be specifically involved in actin ring formation and their function requires microtubules. The sepH gene encodes a protein kinase proposed to be involved in signaling actin ring formation. sepD and sepG are candidate genes for additional components of this signaling pathway. The specific aims of this proposal are to identify the products of these genes and determine their biochemical role in actin ring formation. Specific probes will be developed to investigate actin ring formation in living cells. Novel genetic screens have been devised to identify additional components of this signaling pathway. The central hypothesis to be tested is that these genes represent part of conserved signaling pathway for actin ring formation.

这项研究的长期目标是确定真核细胞如何 在细胞动力学期间的东方和定位收缩肌动蛋白环。这 细胞分裂平面的精确方向可以赋予不同的命运 子细胞对所有真核细胞至关重要。 早就知道有丝分裂主轴的疾病决定了 乳沟的轴，但是，该机制的机制 有丝分裂主轴，即微管，将此信息传达给 该单元是未知的。我们正在研究真菌中的过程， 曲霉尼杜兰人。 A. nidulans作为模型遗传具有丰富的历史 有丝分裂和微管介导的过程的生物体。这 nidulans的细胞被称为septa的横墙和 分隔过程涉及一个收缩的表演环，其形成为 取决于微管。 突变分析已用于定义所需的基因集合 分隔。这些基因的一组，SEPH，SEPD和SEPG，似乎是 特别参与肌动蛋白环形成及其功能需要 微管。 SEPH基因编码提出的蛋白激酶 参与信号肌动蛋白环形成。 SEPD和SEPG是候选人 该信号通路的其他组件的基因。具体 该提案的目的是确定这些基因的产物和 确定其在肌动蛋白环形成中的生化作用。特定探针 将开发用于研究活细胞中肌动蛋白环的形成。 已经设计了新的遗传筛选以识别其他组件 此信号通路。要测试的中心假设是 这些基因代表了肌动蛋白环的保守信号通路的一部分 形成。